##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511707_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2256958 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.04266184838176 31.0 31.0 34.0 28.0 34.0 2 31.225843812778084 31.0 31.0 34.0 28.0 34.0 3 31.341721467568295 33.0 31.0 34.0 28.0 34.0 4 34.90055685573236 37.0 35.0 37.0 32.0 37.0 5 34.55841668298657 37.0 35.0 37.0 31.0 37.0 6 34.50948178920476 37.0 35.0 37.0 31.0 37.0 7 34.344845141114725 37.0 35.0 37.0 30.0 37.0 8 34.254446471755344 37.0 35.0 37.0 30.0 37.0 9 35.77278708775263 39.0 35.0 39.0 30.0 39.0 10 35.588391099878685 39.0 35.0 39.0 30.0 39.0 11 35.59112353885185 39.0 35.0 39.0 30.0 39.0 12 35.411262859122765 39.0 35.0 39.0 30.0 39.0 13 35.396788509134865 39.0 35.0 39.0 30.0 39.0 14 36.44816119750567 40.0 36.0 41.0 30.0 41.0 15 36.36806533395836 40.0 36.0 41.0 29.0 41.0 16 36.20634765910575 40.0 36.0 41.0 29.0 41.0 17 36.17768252665756 40.0 36.0 41.0 29.0 41.0 18 36.09678868636457 39.0 36.0 41.0 29.0 41.0 19 36.11232597150678 39.0 36.0 41.0 28.0 41.0 20 36.21470093816544 40.0 36.0 41.0 29.0 41.0 21 36.16601062137621 40.0 36.0 41.0 29.0 41.0 22 36.06662374753983 40.0 36.0 41.0 29.0 41.0 23 35.962258491296694 40.0 36.0 41.0 28.0 41.0 24 35.81316931905689 40.0 36.0 41.0 27.0 41.0 25 35.28034061776958 39.0 35.0 40.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2542889746174275 1101 2 0.344776099948529 1101 3 0.3988852845149289 1101 4 0.34415388480341136 1101 5 0.5289417227758832 1101 6 0.31338350870738196 1101 7 0.4077809579899352 1101 8 0.2568189675711423 1101 9 0.24003183202014355 1101 10 0.3685165199355467 1101 11 0.4268100600958249 1101 12 0.5720629965676878 1101 13 0.39314235048933455 1101 14 0.37410101698895915 1101 15 0.18128689757838856 1101 16 0.1829331480786749 1101 17 0.04647489772401059 1101 18 0.12175229848340763 1101 19 0.18025790043606804 1101 20 -0.06581156920903908 1101 21 0.06397799484666677 1101 22 0.13195241600326568 1101 23 -0.032256696843454336 1101 24 -0.2546789632198525 1101 25 -0.03400096702517175 1102 1 0.5508991441089499 1102 2 0.535105112909644 1102 3 0.5230698736048041 1102 4 0.5864128222767064 1102 5 0.7428571194430234 1102 6 0.6221429446851587 1102 7 0.8089203968163119 1102 8 0.7344147516474848 1102 9 0.8610259923961578 1102 10 0.8332508882591512 1102 11 0.8810900771874088 1102 12 0.8034830222050644 1102 13 0.9903934543206958 1102 14 0.9469823729211626 1102 15 0.9165098002988046 1102 16 0.7601871005071033 1102 17 0.9207805521891856 1102 18 1.0020684927577292 1102 19 1.2200955304033059 1102 20 1.1062579365593663 1102 21 0.9872510328754345 1102 22 0.923363895849441 1102 23 1.0294154296342555 1102 24 1.0429851871860691 1102 25 1.2704143584270469 1103 1 0.3836762101184341 1103 2 0.4699165668482621 1103 3 0.5385939870063972 1103 4 0.5155859055442704 1103 5 0.5869393716203959 1103 6 0.483982928550887 1103 7 0.4824409452885092 1103 8 0.5634028269703109 1103 9 0.6164471215391671 1103 10 0.7381910783498569 1103 11 0.5725488187836518 1103 12 0.7130636825213799 1103 13 0.5926720713892237 1103 14 0.8279417096437811 1103 15 0.7297531982430812 1103 16 0.8732303354765207 1103 17 0.9556114745126578 1103 18 1.067718684352279 1103 19 1.0248658675794147 1103 20 0.9530387167170034 1103 21 0.9635534619936124 1103 22 1.0541821159261318 1103 23 1.0129197622242145 1103 24 0.9578833978433252 1103 25 1.069169780721957 1104 1 0.2328485766042938 1104 2 0.306561108302418 1104 3 0.40961830489691664 1104 4 0.3785394021541464 1104 5 0.4166252978176317 1104 6 0.4825428546431425 1104 7 0.549628186479076 1104 8 0.6990853726974322 1104 9 0.7142792172829502 1104 10 0.7660132698933211 1104 11 0.6365025964555997 1104 12 0.6889929993860378 1104 13 0.6313548013858181 1104 14 0.8475894709286962 1104 15 0.951658181085115 1104 16 1.0867838893894302 1104 17 0.9705904698373047 1104 18 0.8508648663955256 1104 19 0.8872904130915842 1104 20 0.837233189859667 1104 21 0.8522867354488284 1104 22 0.752855398419868 1104 23 0.9170148843544297 1104 24 0.9743660866235402 1104 25 0.9441130627590013 1105 1 0.5020765160521634 1105 2 0.5154306492158547 1105 3 0.4634177020225536 1105 4 0.4644466044305062 1105 5 0.5717455069951853 1105 6 0.6609763390723771 1105 7 0.7134377882372007 1105 8 0.6676961147274554 1105 9 0.7832660592278202 1105 10 0.895664779952547 1105 11 0.8081526559245731 1105 12 0.7190542184739286 1105 13 0.8282650267410219 1105 14 0.6941960823463944 1105 15 0.6068147420891421 1105 16 0.7339898488251464 1105 17 0.9202198988828911 1105 18 0.9614247019372542 1105 19 0.9901340836944073 1105 20 1.0207589774535535 1105 21 1.1749568663502359 1105 22 1.2211065403461063 1105 23 1.2110369462126158 1105 24 1.191820100474672 1105 25 1.4046604472559707 1106 1 0.169852825861188 1106 2 0.35748224039982546 1106 3 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0.99994322557815 1107 16 1.0040065866955388 1107 17 1.0203530313047082 1107 18 0.9309157593768305 1107 19 0.9566662623572597 1107 20 0.9007951072812119 1107 21 0.8537712600626151 1107 22 0.8986867836659442 1107 23 0.8897582328454376 1107 24 0.9855962667746212 1107 25 0.929631337916156 1108 1 0.3123095290177744 1108 2 0.33987421249526406 1108 3 0.30410695401657506 1108 4 0.33650076296856213 1108 5 0.43246900500686536 1108 6 0.3852486722634936 1108 7 0.4461055253294006 1108 8 0.4982222950995876 1108 9 0.6391205323797848 1108 10 0.7259662361003834 1108 11 0.6622801722659375 1108 12 0.5837494728845485 1108 13 0.5292824738086992 1108 14 0.8044868233826605 1108 15 0.7540139943111441 1108 16 0.8985347985553176 1108 17 0.799976968739557 1108 18 0.7577850651437075 1108 19 0.7001303220063093 1108 20 0.875473138010868 1108 21 0.9037635807192288 1108 22 0.9653467607712614 1108 23 0.8306598491936299 1108 24 1.0146665747470038 1108 25 0.9284026429744898 1109 1 -0.05058602262272416 1109 2 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-1.1093714713944607 2315 5 -1.262390458573627 2315 6 -1.2935652764963663 2315 7 -1.3703639522736921 2315 8 -1.5606875749523894 2315 9 -1.5766981047048532 2315 10 -1.8381049628435022 2315 11 -1.6636500445177589 2315 12 -1.7939057143446036 2315 13 -1.6953734567595546 2315 14 -1.8181807161517725 2315 15 -1.8876625621926522 2315 16 -1.8240494396994862 2315 17 -1.9693739086666469 2315 18 -1.7680255210167815 2315 19 -1.7638403964113891 2315 20 -1.727974503554499 2315 21 -2.036218438555032 2315 22 -1.9664986023595787 2315 23 -1.9834444436143102 2315 24 -1.8809228589980904 2315 25 -2.021381401085847 2316 1 -1.2065072891272983 2316 2 -1.12076935301884 2316 3 -1.1518750008666245 2316 4 -1.1806837899090112 2316 5 -1.137421413402258 2316 6 -1.2742098806929292 2316 7 -1.4328927061048375 2316 8 -1.8523455902316073 2316 9 -1.4244399305875675 2316 10 -1.602228323505365 2316 11 -1.7997638140425494 2316 12 -1.7206198934450896 2316 13 -1.7297629683761713 2316 14 -1.7908356642617633 2316 15 -1.919300439577441 2316 16 -2.0142485845432887 2316 17 -1.794069706163043 2316 18 -1.9835770578835223 2316 19 -1.9040493594647785 2316 20 -1.988630923409879 2316 21 -1.7919836433273844 2316 22 -1.955126242042276 2316 23 -1.9299236505621664 2316 24 -2.1308241838788433 2316 25 -2.0732122506610153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31785.0 3 0.0 4 528.0 5 5063.0 6 6570.0 7 5249.0 8 4183.0 9 4036.0 10 3542.0 11 3588.0 12 3391.0 13 3261.0 14 3203.0 15 3216.0 16 3277.0 17 3433.0 18 3769.0 19 4211.0 20 4998.0 21 6538.0 22 8449.0 23 10689.0 24 13073.0 25 15361.0 26 18248.0 27 20749.0 28 24173.0 29 29563.0 30 37126.0 31 49055.0 32 63312.0 33 82692.0 34 108620.0 35 147486.0 36 219283.0 37 401344.0 38 903894.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.78894647877799 18.90722184328283 13.463887224076876 24.839944453862312 2 15.24893241256594 20.239543668956177 40.453610567852834 24.057913350625046 3 19.661553294301445 23.783960534489342 29.631255876272398 26.92323029493681 4 12.985886312461286 16.786533023653963 37.47867705114584 32.748903612738914 5 13.582831243867002 37.32717913624361 35.541108695093335 13.548880924796055 6 32.68736059776035 36.97893359114347 17.37785993359203 12.955845877504146 7 28.535533226581975 31.247192016865178 21.355337582710888 18.86193717384196 8 25.29360315965118 37.424001687226784 19.845517727844292 17.436877425277743 9 25.943194335029716 15.787090411075438 19.721722779068106 38.547992474826735 10 14.732928127151679 29.633205403024782 34.0066585200079 21.627207949815634 11 35.40057014796022 22.5143755444275 23.42099409913698 18.664060208475302 12 22.977033688708428 24.56027094877264 31.55490709175802 20.90778827076091 13 29.116182046808138 21.449668093070407 25.694541059248778 23.73960880087268 14 20.943810208253765 21.618789538839447 27.52000701829631 29.91739323461048 15 23.342702877058414 29.996038916098573 23.456971729203644 23.20428647763937 16 22.76475680982987 28.334554741381986 26.797928893670154 22.102759555117995 17 22.04418513769419 28.081647952686755 27.437285053598693 22.43688185602036 18 21.72641227705611 27.95102966027724 28.8549454619891 21.467612600677548 19 23.37238885260603 26.87923302072967 27.407599078051074 22.34077904861322 20 23.155282464272705 27.370912529165363 28.046733700848662 21.427071305713266 21 23.31700456986794 26.642188290610637 27.260409808246322 22.780397331275108 22 22.813229134082246 27.405605243872504 27.714339389567726 22.06682623247752 23 22.92771952335843 26.999571990263 27.41455534396298 22.65815314241559 24 22.796170774998913 26.82247520777967 28.126398453139135 22.254955564082273 25 23.064656486384937 26.943762096173884 27.819649148925613 22.171932268515565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 135.0 1 135.0 2 308.5 3 482.0 4 482.0 5 482.0 6 1279.5 7 2077.0 8 2077.0 9 2077.0 10 3148.0 11 4219.0 12 4219.0 13 4219.0 14 6956.0 15 9693.0 16 9693.0 17 9693.0 18 17084.5 19 24476.0 20 24476.0 21 24476.0 22 38967.0 23 53458.0 24 53458.0 25 53458.0 26 76278.5 27 99099.0 28 99099.0 29 99099.0 30 130373.5 31 161648.0 32 161648.0 33 161648.0 34 182810.5 35 203973.0 36 203973.0 37 203973.0 38 222617.0 39 241261.0 40 241261.0 41 241261.0 42 258785.0 43 276309.0 44 276309.0 45 276309.0 46 284038.0 47 291767.0 48 291767.0 49 291767.0 50 285384.5 51 279002.0 52 279002.0 53 279002.0 54 254540.5 55 230079.0 56 230079.0 57 230079.0 58 198905.5 59 167732.0 60 167732.0 61 167732.0 62 138413.5 63 109095.0 64 109095.0 65 109095.0 66 83834.0 67 58573.0 68 58573.0 69 58573.0 70 42962.0 71 27351.0 72 27351.0 73 27351.0 74 19098.0 75 10845.0 76 10845.0 77 10845.0 78 7424.0 79 4003.0 80 4003.0 81 4003.0 82 2622.0 83 1241.0 84 1241.0 85 1241.0 86 797.5 87 354.0 88 354.0 89 354.0 90 214.5 91 75.0 92 75.0 93 75.0 94 41.5 95 8.0 96 8.0 97 8.0 98 5.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4190153294833134 2 0.0 3 0.0 4 0.0 5 0.03190134685714134 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0020381416047618077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2256958.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.90582000743801 #Duplication Level Percentage of deduplicated Percentage of total 1 85.28024784166584 63.87986895017526 2 10.30076777534597 15.431749138369655 3 2.3321630984224204 5.240777678352563 4 0.8420656087300057 2.523024596879341 5 0.39273930008301905 1.4709229660932903 6 0.22861288898781387 1.0274661548340955 7 0.1368227331641319 0.717417331632273 8 0.09470579427578218 0.5675212143746554 9 0.06633410140396016 0.4471929234108163 >10 0.2975514080546814 3.779339069709902 >50 0.015089845004733794 0.7927295678504941 >100 0.01018047820284362 1.5246035970920881 >500 0.0014186747785694133 0.6567743231613685 >1k 0.0012413404312450371 1.6604661156084621 >5k 5.9111449106906534E-5 0.28014637245552754 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6327 0.28033308550712954 No Hit GGTATCAACGCAGAGTACTTTTTTT 3922 0.17377372551903933 No Hit TATCAACGCAGAGTACTTTTTTTTT 3916 0.17350788096189648 No Hit GTCCTACAGTGGACATTTCTAAATT 2662 0.117946368519042 No Hit CTGTAGGACGTGGAATATGGCAAGA 2377 0.1053187520547569 No Hit TTGTAGAACAGTGTATATCAATGAG 2261 0.1001790906166619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.430742619047408E-5 0.0 5 0.0 0.0 0.0 4.430742619047408E-5 0.0 6 0.0 0.0 0.0 4.430742619047408E-5 0.0 7 0.0 0.0 0.0 4.430742619047408E-5 0.0 8 0.0 0.0 0.0 8.861485238094816E-5 0.0 9 0.0 0.0 0.0 8.861485238094816E-5 0.0 10 0.0 0.0 0.0 1.7722970476189632E-4 0.0 11 0.0 0.0 0.0 1.7722970476189632E-4 0.0 12 4.430742619047408E-5 0.0 0.0 1.7722970476189632E-4 4.430742619047408E-5 13 4.430742619047408E-5 4.430742619047408E-5 0.0 2.215371309523704E-4 1.3292227857142225E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 240 0.0 13.059118 19 TCCAACG 325 0.0 12.273201 18 TAGGACC 535 0.0 11.898069 4 ACCGTGC 90 7.4898653E-6 11.607592 8 GGTATCA 1375 0.0 11.51408 1 GTTCACG 60 0.0057082428 11.126808 1 ACCGTCC 60 0.005890331 11.079973 8 TTCGAAA 70 0.0014950805 10.854332 19 ACTGTTC 1040 0.0 10.318986 8 ACTGCGT 120 1.530314E-6 10.288547 8 TGTAGAA 1100 0.0 10.2780285 2 GATATAC 895 0.0 10.229916 1 AAACGGG 75 0.0026540596 10.13071 19 ACCGCTC 85 6.613815E-4 10.055774 8 AGGACGT 1255 0.0 9.765657 5 TGTAGGA 1480 0.0 9.757474 2 GACGTGG 675 0.0 9.708167 7 GTATCAA 2565 0.0 9.704585 1 GGACGTG 1230 0.0 9.651545 6 TAGAACA 1145 0.0 9.625161 4 >>END_MODULE