FastQCFastQC Report
Thu 26 May 2016
SRR1511706_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511706_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346408
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56980.24283926751016874No Hit
TATCAACGCAGAGTACTTTTTTTTT35290.15040010092021508No Hit
GGTATCAACGCAGAGTACTTTTTTT34930.1488658408938258No Hit
GTCCTACAGTGGACATTTCTAAATT27000.11506950197919542No Hit
GTCCTAAAGTGTGTATTTCTCATTT23630.10070712339882919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG551.4015587E-414.375461
CCAACGA2350.013.2798619
GCGTATA450.00886284313.1775041
GGTATCA11900.012.20857051
TCCAACG4200.011.9336718
TAGGACC6150.011.5327684
TATCGCG803.934169E-410.6389787
CGGTTTC2500.010.59169413
CCCGTTT3400.010.56944412
GGTCTAC2650.010.4425521
GATATAC9300.010.3082091
TGGACCG1201.6160848E-610.2449435
GGCGAGG6200.010.06699219
GAACAGT6500.010.0388316
GTATCAA23950.09.9862941
TCCCGTT3700.09.96805311
ATCGCGC951.7218855E-49.95468
CCGTTTC3250.09.89334113
CGTTTCC3350.09.88031214
CGCGCGC1151.0838947E-59.86803810
Produced by FastQC (version 0.11.5)