##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511706_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346408 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.31894112191912 31.0 30.0 34.0 23.0 34.0 2 26.669131711109067 31.0 26.0 34.0 10.0 34.0 3 28.323996508706074 31.0 27.0 34.0 16.0 34.0 4 32.81413121673639 35.0 32.0 37.0 26.0 37.0 5 33.26394386653983 35.0 35.0 37.0 28.0 37.0 6 33.511327953194844 37.0 35.0 37.0 28.0 37.0 7 33.54952207800178 37.0 35.0 37.0 28.0 37.0 8 33.57196531890447 37.0 35.0 37.0 28.0 37.0 9 35.16016651835486 39.0 35.0 39.0 29.0 39.0 10 35.034063982052565 39.0 35.0 39.0 28.0 39.0 11 35.0470425433258 39.0 35.0 39.0 28.0 39.0 12 34.94544640147834 39.0 35.0 39.0 27.0 39.0 13 34.9416550744798 39.0 35.0 39.0 27.0 39.0 14 36.08452536813717 40.0 36.0 41.0 27.0 41.0 15 36.01733628593152 40.0 36.0 41.0 27.0 41.0 16 35.95440733239914 40.0 36.0 41.0 27.0 41.0 17 35.92297844194189 40.0 36.0 41.0 27.0 41.0 18 35.862996972393546 40.0 36.0 41.0 27.0 41.0 19 35.8954048059843 40.0 36.0 41.0 27.0 41.0 20 35.81824772162386 40.0 36.0 41.0 27.0 41.0 21 35.73374110555368 40.0 36.0 41.0 27.0 41.0 22 35.62242457407237 40.0 36.0 41.0 27.0 41.0 23 35.51860886938674 40.0 36.0 41.0 27.0 41.0 24 35.402674215226 40.0 36.0 41.0 26.0 41.0 25 35.291524747614226 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0507681352937226 1101 2 3.5914485876045283 1101 3 2.2205315631235045 1101 4 1.1909249330873592 1101 5 0.696698193857813 1101 6 0.48903781175852856 1101 7 0.49399313579903037 1101 8 0.30615082551060624 1101 9 0.2731119381328284 1101 10 0.49237674808829013 1101 11 0.33589920663362705 1101 12 0.4165199427854702 1101 13 0.5589540637212593 1101 14 0.5955296629787554 1101 15 0.4970520609641085 1101 16 0.5355867219303789 1101 17 0.5320608031753693 1101 18 0.5124309149619464 1101 19 0.7208999855332152 1101 20 0.5802337430099911 1101 21 0.5801539933570368 1101 22 0.46689706397806674 1101 23 0.6915119484751031 1101 24 0.6875085089352808 1101 25 0.5650439405677261 1102 1 1.5423983181420375 1102 2 4.161513063618013 1102 3 2.6929841341596443 1102 4 1.6705192840698118 1102 5 1.2926888020297298 1102 6 1.053472527184745 1102 7 0.9327620360410194 1102 8 0.9699347485013234 1102 9 1.12718571316978 1102 10 1.0426917037856853 1102 11 1.1261166710720545 1102 12 1.0420163895283778 1102 13 1.178856015634544 1102 14 1.2276744010725054 1102 15 1.2682431851297338 1102 16 1.2584066832772507 1102 17 1.3312995732356683 1102 18 1.2279248791811241 1102 19 1.2283104348700107 1102 20 1.36155820422114 1102 21 1.4670239949579198 1102 22 1.08170984224693 1102 23 1.2417893182992117 1102 24 1.2055126062594894 1102 25 0.9976216122388806 1103 1 1.349408145590175 1103 2 3.876450668925628 1103 3 2.5654884467306935 1103 4 1.385211793631889 1103 5 1.1036239492498296 1103 6 0.8408160948251577 1103 7 0.6469935427871718 1103 8 0.4812113809392571 1103 9 0.5460866503713433 1103 10 0.4352608909724367 1103 11 0.5304395361432839 1103 12 0.7404356556582385 1103 13 0.755460690902737 1103 14 0.9636911317537198 1103 15 0.704601401028782 1103 16 0.6806389172893219 1103 17 0.8469522788030375 1103 18 0.8172687400451721 1103 19 0.8115110822792815 1103 20 0.7238738866501322 1103 21 0.8916263048293516 1103 22 1.0275421227826342 1103 23 0.8482803003354746 1103 24 1.0957036962715705 1103 25 0.7789534268698475 1104 1 1.6408408960196574 1104 2 4.169956484143704 1104 3 2.5448018650387 1104 4 1.8150786008472863 1104 5 1.3174396344704604 1104 6 1.1861022928156473 1104 7 1.1616339387664212 1104 8 1.0872899976341373 1104 9 1.1324574758592334 1104 10 1.05595298636554 1104 11 1.161336754242292 1104 12 1.1897734254225796 1104 13 1.168836321993581 1104 14 1.30434842570736 1104 15 1.3588647727308185 1104 16 1.1699824959765692 1104 17 1.1663559915643873 1104 18 1.2071796655524665 1104 19 1.3846681276822252 1104 20 1.380338397300477 1104 21 1.4509285481242316 1104 22 1.4346269248081995 1104 23 1.5015160397119445 1104 24 1.445455340939148 1104 25 1.4594240277720942 1105 1 1.6038596978192494 1105 2 3.89866528727406 1105 3 2.544447270058079 1105 4 1.559472652578478 1105 5 1.1242837308773161 1105 6 0.9367385387045388 1105 7 0.816099314508989 1105 8 0.7865025322774031 1105 9 0.8950730808486895 1105 10 0.7395666481083794 1105 11 0.8202300211531224 1105 12 0.9807611749512333 1105 13 0.9628331694871619 1105 14 0.9549434261661034 1105 15 0.9445461612883932 1105 16 0.8535060624912276 1105 17 1.066318878942873 1105 18 1.070926337608313 1105 19 1.1773825646573073 1105 20 1.045596189504515 1105 21 1.0762278667894023 1105 22 1.0074102309063235 1105 23 0.9842305676088117 1105 24 1.0918609132876824 1105 25 1.0921177676415468 1106 1 1.1158956495781105 1106 2 3.6273288586406487 1106 3 2.1238994267366422 1106 4 1.293522927560673 1106 5 0.7940562570819978 1106 6 0.7742981824557944 1106 7 0.5329119542784042 1106 8 0.5787111174883677 1106 9 0.4287570801857399 1106 10 0.3152970530729817 1106 11 0.42024833952645224 1106 12 0.46510355682641347 1106 13 0.5723795670803327 1106 14 0.6263806747188809 1106 15 0.7299025730174833 1106 16 0.7191492876084311 1106 17 0.5752641216980479 1106 18 0.6673923147879322 1106 19 0.41576589245839557 1106 20 0.6256073090784966 1106 21 0.6929312849795011 1106 22 0.6523817915169587 1106 23 0.604662624119392 1106 24 0.6614645339899283 1106 25 0.5503142296704056 1107 1 1.5808450235795668 1107 2 3.930671392498976 1107 3 2.6327432320515918 1107 4 1.6729203613514443 1107 5 1.2196705556361493 1107 6 1.1807610139294908 1107 7 0.948347897616479 1107 8 0.9757023308830028 1107 9 1.04783292777919 1107 10 0.9913267156949743 1107 11 1.073513347232847 1107 12 0.9738976732975289 1107 13 1.0367273594050062 1107 14 1.1035717620656342 1107 15 1.2631796539573728 1107 16 1.2728977099876388 1107 17 1.2436160490589714 1107 18 1.1217406317343475 1107 19 1.1449101558120418 1107 20 1.3083988144586414 1107 21 1.3313855505587426 1107 22 1.24603821326847 1107 23 1.4540726095432248 1107 24 1.4381166476031169 1107 25 1.4764622788517343 1108 1 1.415298814195662 1108 2 4.0129300192656 1108 3 2.5137969803410165 1108 4 1.5496271145440375 1108 5 1.1120844098428364 1108 6 1.0003606206861306 1108 7 0.8358603315584432 1108 8 0.76375019328961 1108 9 0.8535496586903122 1108 10 0.7560807896366484 1108 11 0.7622301179944841 1108 12 0.7353840047633042 1108 13 0.7552321989215542 1108 14 0.8479821465689668 1108 15 0.7757167042994055 1108 16 0.8662864316720089 1108 17 0.8449771957324756 1108 18 0.9265224230654283 1108 19 0.8675225004192058 1108 20 0.8497210933356669 1108 21 0.7690363199639165 1108 22 0.9902181998740502 1108 23 1.0877970196104698 1108 24 1.021287773852272 1108 25 0.9913193644479392 1109 1 1.0647365346525497 1109 2 3.0391517751062054 1109 3 1.8931825057996363 1109 4 1.1245179948920097 1109 5 0.8044840653716463 1109 6 0.5398659928134109 1109 7 0.510651447419562 1109 8 0.3369912606331553 1109 9 0.32067298670143174 1109 10 0.2939446085982169 1109 11 0.4496598644748957 1109 12 0.4126764152643574 1109 13 0.35890682160852805 1109 14 0.5037178144918428 1109 15 0.34875019830455756 1109 16 0.47144926182314606 1109 17 0.3591643665509707 1109 18 0.3196063523640831 1109 19 0.4633118501113245 1109 20 0.4875824702579621 1109 21 0.5956242380464261 1109 22 0.4287757734638262 1109 23 0.5296815125892493 1109 24 0.4788706290127678 1109 25 0.6002741788206336 1110 1 0.9287349960192053 1110 2 3.3926238336073595 1110 3 1.9778983342833776 1110 4 1.06248563395841 1110 5 0.6778125594549422 1110 6 0.41889301402752466 1110 7 0.2817714649566696 1110 8 0.15945385984812077 1110 9 0.08451138056675234 1110 10 0.350411831985447 1110 11 0.2081068374619477 1110 12 0.3302902513833672 1110 13 0.15030412403683613 1110 14 0.2071431349370556 1110 15 0.1091680282524905 1110 16 0.234930916102158 1110 17 0.29084408264829875 1110 18 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-1.961907244449968 2315 16 -2.2337248625401287 2315 17 -1.8982771269180674 2315 18 -2.006223680954264 2315 19 -2.0564894128830744 2315 20 -2.0787269355105877 2315 21 -2.067883622895735 2315 22 -2.0601487574442885 2315 23 -2.0393768547853384 2315 24 -1.9343353843022584 2315 25 -2.0060892413234015 2316 1 -0.531488162625223 2316 2 1.8313382973737546 2316 3 0.36335741197344973 2316 4 -0.5266897869369984 2316 5 -0.6290216740307244 2316 6 -0.919380276177634 2316 7 -1.2843412618124717 2316 8 -1.3460687230944899 2316 9 -1.3219004737470499 2316 10 -1.492529391034715 2316 11 -1.4882589211389572 2316 12 -1.7813968912759464 2316 13 -1.5930832184365045 2316 14 -1.8647493611556882 2316 15 -1.76236341772848 2316 16 -2.0104911663161857 2316 17 -1.8945932331172273 2316 18 -2.1190432442230573 2316 19 -2.0576416291529753 2316 20 -1.8453833621251832 2316 21 -2.196206344449081 2316 22 -1.9003761707650497 2316 23 -2.1845426384228617 2316 24 -2.09850053249545 2316 25 -2.0390927888702137 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83126.0 3 2.0 4 743.0 5 3768.0 6 4350.0 7 4004.0 8 3659.0 9 3487.0 10 3421.0 11 3371.0 12 3365.0 13 3198.0 14 3303.0 15 3343.0 16 3502.0 17 3865.0 18 4419.0 19 5477.0 20 7169.0 21 9216.0 22 11208.0 23 13307.0 24 15616.0 25 17899.0 26 19969.0 27 23271.0 28 26881.0 29 32215.0 30 40204.0 31 51921.0 32 66539.0 33 88286.0 34 120791.0 35 173024.0 36 276455.0 37 350017.0 38 862017.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57997415624222 18.463753959243235 12.81968012383183 25.136591760682713 2 16.632950293960448 21.052690183502584 39.43684304293604 22.877516479600928 3 18.9599166044439 24.44885970385372 29.56097149344871 27.030252198253674 4 12.302293548266116 16.93000535286276 38.32291741248751 32.44478368638361 5 13.425712834255593 37.461217315999605 35.77246582861975 13.340604021125055 6 31.629963757368714 37.09623390305523 18.34821565558931 12.92558668398676 7 27.738782002107047 31.415295208676415 22.367721214724806 18.478201574491735 8 24.894519623185737 36.913486486578634 20.774392177319545 17.417601712916085 9 26.284218260421888 15.460099010913703 20.12744586619207 38.12823686247234 10 14.878699697580302 29.069411628327213 34.39529698159911 21.656591692493375 11 34.546336357530315 22.541774491051854 24.3373701419361 18.574519009481726 12 22.988158922063 24.54027603042608 31.944316589442245 20.527248458068673 13 29.167092850007332 21.67308498777706 25.84644273289215 23.31337942932346 14 21.247967105465033 21.70432422664771 27.73541515371581 29.31229351417145 15 23.352417823328253 30.00633308444226 23.708110439446166 22.93313865278332 16 22.546547744467286 27.934741102144212 27.260220728875794 22.258490424512704 17 21.886730696451767 27.92344724361663 27.78221008452068 22.407611975410926 18 21.736884633874414 27.544144070425947 29.25535541985878 21.463615875840862 19 23.400671750467843 26.755716293038677 27.46637731646726 22.37723464002622 20 23.312792708170406 27.273103089106026 28.007118969556434 21.406985233167138 21 23.33263012992216 26.676000093760454 27.241887057008483 22.7494827193089 22 23.041365848669013 27.412354350105268 27.510121975127642 22.036157826098073 23 22.975431610018628 27.043384536835113 27.400358593566537 22.580825259579722 24 23.096632425661873 26.67623306613047 27.997146275134703 22.22998823307295 25 23.133615296231515 26.931974319896625 27.77841705278877 22.155993331083085 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 352.0 1 352.0 2 501.0 3 650.0 4 650.0 5 650.0 6 1632.5 7 2615.0 8 2615.0 9 2615.0 10 3712.5 11 4810.0 12 4810.0 13 4810.0 14 7926.5 15 11043.0 16 11043.0 17 11043.0 18 19189.5 19 27336.0 20 27336.0 21 27336.0 22 43204.0 23 59072.0 24 59072.0 25 59072.0 26 84593.0 27 110114.0 28 110114.0 29 110114.0 30 142551.5 31 174989.0 32 174989.0 33 174989.0 34 195681.0 35 216373.0 36 216373.0 37 216373.0 38 233172.5 39 249972.0 40 249972.0 41 249972.0 42 266584.5 43 283197.0 44 283197.0 45 283197.0 46 291424.0 47 299651.0 48 299651.0 49 299651.0 50 291782.0 51 283913.0 52 283913.0 53 283913.0 54 257926.5 55 231940.0 56 231940.0 57 231940.0 58 200923.5 59 169907.0 60 169907.0 61 169907.0 62 140874.0 63 111841.0 64 111841.0 65 111841.0 66 86725.5 67 61610.0 68 61610.0 69 61610.0 70 45251.5 71 28893.0 72 28893.0 73 28893.0 74 20463.0 75 12033.0 76 12033.0 77 12033.0 78 8122.5 79 4212.0 80 4212.0 81 4212.0 82 2780.5 83 1349.0 84 1349.0 85 1349.0 86 873.5 87 398.0 88 398.0 89 398.0 90 247.5 91 97.0 92 97.0 93 97.0 94 65.5 95 34.0 96 34.0 97 34.0 98 20.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.313316354189041 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.261833406636868E-5 20 4.261833406636868E-5 21 1.2785500219910604E-4 22 0.0011933133538583232 23 8.097483472610049E-4 24 4.261833406636868E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2346408.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.1430989675607 #Duplication Level Percentage of deduplicated Percentage of total 1 81.21748599786906 57.780636442428744 2 12.793278449811169 18.203069497489548 3 3.3578127928576986 7.1665562351045 4 1.2067202279808846 3.4339926642160594 5 0.5221669699781923 1.8574288211374919 6 0.2738169207209966 1.168811057390795 7 0.1596151419467465 0.7948861088167046 8 0.10101039334474411 0.5748953928381892 9 0.06664037054165543 0.4266902229011919 >10 0.26637804647847435 3.2464476993340603 >50 0.017928083506415296 0.8777921942012373 >100 0.014302624472337393 2.016257745060141 >500 0.001754796473525777 0.8432838833624492 >1k 0.0010286737948187778 1.363959699848019 >5k 6.051022322463398E-5 0.2452923358709132 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5698 0.24283926751016874 No Hit TATCAACGCAGAGTACTTTTTTTTT 3529 0.15040010092021508 No Hit GGTATCAACGCAGAGTACTTTTTTT 3493 0.1488658408938258 No Hit GTCCTACAGTGGACATTTCTAAATT 2700 0.11506950197919542 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2363 0.10070712339882919 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.261833406636868E-5 0.0 0.0 0.0 6 0.0 4.261833406636868E-5 0.0 0.0 0.0 7 0.0 4.261833406636868E-5 0.0 0.0 0.0 8 0.0 4.261833406636868E-5 0.0 0.0 0.0 9 0.0 4.261833406636868E-5 0.0 0.0 0.0 10 0.0 4.261833406636868E-5 0.0 4.261833406636868E-5 0.0 11 0.0 4.261833406636868E-5 0.0 8.523666813273736E-5 0.0 12 0.0 4.261833406636868E-5 0.0 8.523666813273736E-5 0.0 13 0.0 4.261833406636868E-5 0.0 8.523666813273736E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 55 1.4015587E-4 14.37546 1 CCAACGA 235 0.0 13.27986 19 GCGTATA 45 0.008862843 13.177504 1 GGTATCA 1190 0.0 12.2085705 1 TCCAACG 420 0.0 11.93367 18 TAGGACC 615 0.0 11.532768 4 TATCGCG 80 3.934169E-4 10.638978 7 CGGTTTC 250 0.0 10.591694 13 CCCGTTT 340 0.0 10.569444 12 GGTCTAC 265 0.0 10.442552 1 GATATAC 930 0.0 10.308209 1 TGGACCG 120 1.6160848E-6 10.244943 5 GGCGAGG 620 0.0 10.066992 19 GAACAGT 650 0.0 10.038831 6 GTATCAA 2395 0.0 9.986294 1 TCCCGTT 370 0.0 9.968053 11 ATCGCGC 95 1.7218855E-4 9.9546 8 CCGTTTC 325 0.0 9.893341 13 CGTTTCC 335 0.0 9.880312 14 CGCGCGC 115 1.0838947E-5 9.868038 10 >>END_MODULE