FastQCFastQC Report
Thu 26 May 2016
SRR1511706_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511706_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2346408
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68470.2918077333524264No Hit
TATCAACGCAGAGTACTTTTTTTTT44310.18884183824807962No Hit
GGTATCAACGCAGAGTACTTTTTTT43660.18607164653376565No Hit
GTCCTACAGTGGACATTTCTAAATT29090.12397673379906649No Hit
GTCCTAAAGTGTGTATTTCTCATTT26200.11166003525388593No Hit
CTGTAGGACGTGGAATATGGCAAGA25240.10756867518351455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCG350.002169374116.287145
ACCGACC400.005290549214.245788
GGTATCA14400.012.1189251
GTACCGA550.003076062512.0873296
CCAACGA2300.011.97471419
CCGGTTT2600.011.68884712
GGACCGA658.0460584E-411.6888476
TAGGACC5050.011.4762524
TCCAACG3750.011.396625518
GCCGGTT2700.011.25592511
TGGACCG1451.2169039E-911.1389065
AAGACCG600.00587334511.0843045
CCATGCG600.005890140311.0800519
TGCCGGT2850.010.99674310
ACTGTTC9500.010.7968028
CCTATAC2050.010.6594693
TACACCG909.510458E-510.5564795
TATCGCG1002.4076577E-510.4469067
TAGACAG3650.010.411875
GTCCTAC15900.010.2559181
Produced by FastQC (version 0.11.5)