##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511706_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2346408 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.437522374625384 33.0 31.0 34.0 28.0 34.0 2 31.60902792694195 33.0 31.0 34.0 28.0 34.0 3 31.725814521600675 34.0 31.0 34.0 28.0 34.0 4 35.261992799206276 37.0 35.0 37.0 32.0 37.0 5 34.96148069730413 37.0 35.0 37.0 32.0 37.0 6 34.92961326418935 37.0 35.0 37.0 32.0 37.0 7 34.78317368505392 37.0 35.0 37.0 32.0 37.0 8 34.70976744027467 37.0 35.0 37.0 32.0 37.0 9 36.294270646878125 39.0 37.0 39.0 32.0 39.0 10 36.12743606397523 39.0 37.0 39.0 32.0 39.0 11 36.132197810440466 39.0 37.0 39.0 32.0 39.0 12 35.98393160950696 39.0 37.0 39.0 31.0 39.0 13 35.96939918377367 39.0 37.0 39.0 31.0 39.0 14 37.10283292590206 40.0 37.0 41.0 31.0 41.0 15 37.03469558576343 40.0 37.0 41.0 31.0 41.0 16 36.887243395010586 40.0 37.0 41.0 31.0 41.0 17 36.87084428624519 40.0 37.0 41.0 31.0 41.0 18 36.80533777586847 40.0 37.0 41.0 31.0 41.0 19 36.823161615541714 40.0 37.0 41.0 31.0 41.0 20 36.91980422842063 40.0 37.0 41.0 31.0 41.0 21 36.873161018885035 40.0 37.0 41.0 31.0 41.0 22 36.78638625507585 40.0 37.0 41.0 31.0 41.0 23 36.69727259709309 40.0 37.0 41.0 30.0 41.0 24 36.56428293800567 40.0 37.0 41.0 30.0 41.0 25 36.08131279811525 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12888551344695287 1101 2 0.165780534143515 1101 3 0.21190770931728053 1101 4 0.13854088433090084 1101 5 0.3212892554744826 1101 6 0.12071508073567117 1101 7 0.2503526252170616 1101 8 0.19437802342197585 1101 9 0.19541491299050762 1101 10 0.2943675564004735 1101 11 0.35163539069137784 1101 12 0.3054432482692988 1101 13 0.3526519186893893 1101 14 0.13503180506771173 1101 15 0.21466932359455626 1101 16 -0.02270516132767142 1101 17 -0.10502161635660201 1101 18 0.23306577482186697 1101 19 0.20825198412017443 1101 20 0.016840434195870557 1101 21 0.2470181698001923 1101 22 0.003278760124594271 1101 23 0.2441718489841591 1101 24 -0.15954947674665476 1101 25 0.21868518625307587 1102 1 0.3780114098816334 1102 2 0.39846469912229665 1102 3 0.4095326069421752 1102 4 0.438027210729409 1102 5 0.5184902679729504 1102 6 0.47980656985549075 1102 7 0.5158919476968933 1102 8 0.5273048724961313 1102 9 0.4082606972243923 1102 10 0.7070627651296704 1102 11 0.6179681927182301 1102 12 0.7244368749116461 1102 13 0.7296447240108037 1102 14 0.7981700398773341 1102 15 0.7817990853418806 1102 16 0.6654170066811815 1102 17 0.8273600055434329 1102 18 0.8832387394424259 1102 19 0.9645024916454688 1102 20 0.7000414049322501 1102 21 0.6916089362039912 1102 22 0.7432961145895405 1102 23 0.8901890224800866 1102 24 0.9417528041590302 1102 25 0.9810819121058927 1103 1 0.24311583626623445 1103 2 0.2578124948871334 1103 3 0.30610331639632093 1103 4 0.35462491636585725 1103 5 0.4252848122307782 1103 6 0.3889916888895826 1103 7 0.3378207531759898 1103 8 0.425714022856134 1103 9 0.422072288114137 1103 10 0.6212404845003903 1103 11 0.3613455942666093 1103 12 0.44045802622886043 1103 13 0.5367552635289314 1103 14 0.7038170904174095 1103 15 0.6067853488394164 1103 16 0.6934555143422116 1103 17 0.7463112987799931 1103 18 0.6936522783531558 1103 19 0.8046064835421589 1103 20 0.6888706092935308 1103 21 0.795140052891405 1103 22 0.6670492791098326 1103 23 0.7445397616220149 1103 24 0.612563371439812 1103 25 0.7521462203276101 1104 1 0.4192955186683669 1104 2 0.4654595641813337 1104 3 0.4526175502111123 1104 4 0.4270567147050599 1104 5 0.523489255926556 1104 6 0.6056313007944851 1104 7 0.6508320458436785 1104 8 0.6458989011618002 1104 9 0.7155001734621464 1104 10 0.7222352082791588 1104 11 0.7518853856055827 1104 12 0.7892638757881727 1104 13 0.7659671993459796 1104 14 0.9447669144348367 1104 15 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0.8728635309591368 1107 15 0.8955792701761425 1107 16 1.0509731302610703 1107 17 1.141685878112085 1107 18 0.992132847321777 1107 19 1.0507862415798073 1107 20 1.0621421809901577 1107 21 0.9415259747557627 1107 22 0.9496574318763962 1107 23 0.952756688912288 1107 24 1.0424430177831567 1107 25 0.8980109163250773 1108 1 0.2096544774494511 1108 2 0.17209833028167054 1108 3 0.060658925733168445 1108 4 0.17648675461209962 1108 5 0.3039824160119551 1108 6 0.3044082770037164 1108 7 0.2540316133271858 1108 8 0.22549893358480233 1108 9 0.16903376864528497 1108 10 0.4373318909815751 1108 11 0.3209951618475557 1108 12 0.3513574570577589 1108 13 0.3642860039761686 1108 14 0.3471614944429078 1108 15 0.4785896402094849 1108 16 0.6243526360295277 1108 17 0.657006661240537 1108 18 0.5686893679304958 1108 19 0.5712941693180511 1108 20 0.5402460623919154 1108 21 0.5633522969127611 1108 22 0.4127786792053527 1108 23 0.398412963105514 1108 24 0.4175741279218599 1108 25 0.4352133093680237 1109 1 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-0.7553144261949143 2314 20 -0.6896794744329995 2314 21 -0.7238458400796333 2314 22 -0.659600639485916 2314 23 -0.4899796215469223 2314 24 -0.6978256023690008 2314 25 -0.8499934300529546 2315 1 -0.6665473626705172 2315 2 -0.6470489148717533 2315 3 -0.6376789427143592 2315 4 -0.6857761453651037 2315 5 -0.6981561639709355 2315 6 -0.7990498434302324 2315 7 -0.898701690585419 2315 8 -0.8502371248606551 2315 9 -0.8810382842450863 2315 10 -0.9208703214290921 2315 11 -0.891479295455099 2315 12 -1.086121758454631 2315 13 -0.9832128509162104 2315 14 -1.254137130800494 2315 15 -1.2364720282802892 2315 16 -1.0743209215008847 2315 17 -1.306746238130124 2315 18 -1.2807901571269724 2315 19 -1.236207488212159 2315 20 -1.1706649831383942 2315 21 -1.1935063225580365 2315 22 -1.3930811345238112 2315 23 -1.2526450326737972 2315 24 -1.3546299794066172 2315 25 -1.3236594446001106 2316 1 -0.8254336938842997 2316 2 -0.9619725984288934 2316 3 -0.8989585003441114 2316 4 -0.8656169897064885 2316 5 -1.0076226011482134 2316 6 -1.0212979697111138 2316 7 -1.0871929942562701 2316 8 -1.8486969088095861 2316 9 -1.3817768304663005 2316 10 -1.4694548797111224 2316 11 -1.3993971001001526 2316 12 -1.47665742539764 2316 13 -1.648905169614686 2316 14 -1.6038882133703822 2316 15 -1.740122676980576 2316 16 -1.6734809566817788 2316 17 -1.685933963534012 2316 18 -1.6112296236903987 2316 19 -1.751524221439162 2316 20 -1.7701860853607698 2316 21 -1.6233730475789727 2316 22 -1.7252973202587185 2316 23 -1.62389938294249 2316 24 -1.7511026321552308 2316 25 -1.8561962982669584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23718.0 3 1.0 4 394.0 5 4000.0 6 5246.0 7 4204.0 8 3526.0 9 3253.0 10 2907.0 11 2871.0 12 2860.0 13 2737.0 14 2705.0 15 2719.0 16 2767.0 17 2910.0 18 3261.0 19 3545.0 20 4296.0 21 5567.0 22 7165.0 23 9286.0 24 11422.0 25 13618.0 26 16105.0 27 18323.0 28 21597.0 29 26507.0 30 34127.0 31 44552.0 32 58453.0 33 76610.0 34 102270.0 35 139279.0 36 207991.0 37 383759.0 38 1091857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.66794903402982 18.921681425020033 13.286413069532047 25.1239564714181 2 14.979364202645066 20.5705486854801 40.547892779090425 23.90219433278441 3 18.86330084111544 24.604629714866295 29.941894163333917 26.59017528068435 4 12.575477069631539 17.311354206088623 37.98853396340278 32.12463476087705 5 13.344210255712834 37.630470843978976 35.76313320165635 13.262185698651837 6 31.614876867109214 37.36055281093484 18.146801408791653 12.877768913164292 7 27.77730897610305 31.448281799243784 22.354935714504894 18.419473510148276 8 24.99416128823291 36.93679871531294 20.673088397243788 17.39595159921037 9 26.228516097797144 15.411173163405511 20.052608071571527 38.30770266722582 10 14.882918912652872 29.20361676230221 34.312148611835624 21.601315713209296 11 34.68130862151851 22.483813556721593 24.22374966331516 18.611128158444735 12 22.90189941391267 24.58958544294087 31.933363677587188 20.575151465559273 13 29.184182801967946 21.669377192713288 25.877937681767193 23.268502323551573 14 21.21954067664277 21.69187967310033 27.759068329122645 29.329511321134262 15 23.34180585814573 30.0103392078445 23.711392051169277 22.936462882840495 16 22.646999157861718 27.905249214970283 27.229322436677677 22.218429190490316 17 21.86993907282962 27.960951377595027 27.74815803560165 22.4209515139737 18 21.817518521927987 27.461890685677854 29.23038107609589 21.490209716298274 19 23.419754791153117 26.744666741674934 27.499650529660656 22.335927937511293 20 23.317428171059763 27.239124653512942 28.00480564334932 21.438641532077966 21 23.34163538480946 26.58936553233709 27.263885905605505 22.805113177247947 22 23.006484805711537 27.34554263367667 27.585441236136255 22.06253132447554 23 22.947501031363686 26.99176784259174 27.51614382494434 22.544587301100236 24 23.06904852012097 26.67183200875551 28.03617273722217 22.22294673390135 25 23.10156685515753 26.88688332034866 27.870749366251328 22.14080045824248 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 296.0 1 296.0 2 463.0 3 630.0 4 630.0 5 630.0 6 1673.0 7 2716.0 8 2716.0 9 2716.0 10 3722.0 11 4728.0 12 4728.0 13 4728.0 14 7582.5 15 10437.0 16 10437.0 17 10437.0 18 18983.5 19 27530.0 20 27530.0 21 27530.0 22 43615.0 23 59700.0 24 59700.0 25 59700.0 26 85368.0 27 111036.0 28 111036.0 29 111036.0 30 143273.5 31 175511.0 32 175511.0 33 175511.0 34 195836.0 35 216161.0 36 216161.0 37 216161.0 38 233278.0 39 250395.0 40 250395.0 41 250395.0 42 266881.5 43 283368.0 44 283368.0 45 283368.0 46 290925.5 47 298483.0 48 298483.0 49 298483.0 50 291211.0 51 283939.0 52 283939.0 53 283939.0 54 258113.0 55 232287.0 56 232287.0 57 232287.0 58 201477.0 59 170667.0 60 170667.0 61 170667.0 62 140825.0 63 110983.0 64 110983.0 65 110983.0 66 86272.5 67 61562.0 68 61562.0 69 61562.0 70 45147.0 71 28732.0 72 28732.0 73 28732.0 74 20103.5 75 11475.0 76 11475.0 77 11475.0 78 7786.5 79 4098.0 80 4098.0 81 4098.0 82 2660.5 83 1223.0 84 1223.0 85 1223.0 86 795.5 87 368.0 88 368.0 89 368.0 90 220.0 91 72.0 92 72.0 93 72.0 94 41.0 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4306156474065891 2 0.0 3 0.0 4 0.0 5 0.03277349889703751 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0022161533714511713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2346408.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.21626176958641 #Duplication Level Percentage of deduplicated Percentage of total 1 79.07406275556188 53.941369641181126 2 13.974209321391875 19.06536642182125 3 3.8534539980825038 7.88604679950766 4 1.4140221951204237 3.8583723284136 5 0.6550097346259175 2.234115775943445 6 0.3039370249805391 1.2440068594525067 7 0.18549886695904183 0.8857827486507777 8 0.11322146121352424 0.6178835868861474 9 0.07838626452734178 0.4812496146123472 >10 0.3066335569823398 3.574100266852519 >50 0.02218179290789317 1.0391091314218273 >100 0.016073089989516794 2.22422749722788 >500 0.0020609045790068786 0.9920770520591984 >1k 0.0011865814242750551 1.6645951241065025 >5k 6.245165390921343E-5 0.291697151863364 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6847 0.2918077333524264 No Hit TATCAACGCAGAGTACTTTTTTTTT 4431 0.18884183824807962 No Hit GGTATCAACGCAGAGTACTTTTTTT 4366 0.18607164653376565 No Hit GTCCTACAGTGGACATTTCTAAATT 2909 0.12397673379906649 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2620 0.11166003525388593 No Hit CTGTAGGACGTGGAATATGGCAAGA 2524 0.10756867518351455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.261833406636868E-5 0.0 2 0.0 0.0 0.0 4.261833406636868E-5 0.0 3 0.0 4.261833406636868E-5 0.0 4.261833406636868E-5 0.0 4 0.0 4.261833406636868E-5 0.0 8.523666813273736E-5 0.0 5 0.0 8.523666813273736E-5 0.0 8.523666813273736E-5 0.0 6 0.0 8.523666813273736E-5 0.0 8.523666813273736E-5 0.0 7 0.0 8.523666813273736E-5 0.0 8.523666813273736E-5 0.0 8 0.0 8.523666813273736E-5 0.0 8.523666813273736E-5 0.0 9 0.0 8.523666813273736E-5 0.0 8.523666813273736E-5 0.0 10 0.0 8.523666813273736E-5 0.0 1.2785500219910604E-4 0.0 11 0.0 8.523666813273736E-5 0.0 1.2785500219910604E-4 0.0 12 0.0 8.523666813273736E-5 0.0 1.2785500219910604E-4 0.0 13 0.0 8.523666813273736E-5 0.0 1.7047333626547473E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACCCG 35 0.0021693741 16.28714 5 ACCGACC 40 0.0052905492 14.24578 8 GGTATCA 1440 0.0 12.118925 1 GTACCGA 55 0.0030760625 12.087329 6 CCAACGA 230 0.0 11.974714 19 CCGGTTT 260 0.0 11.688847 12 GGACCGA 65 8.0460584E-4 11.688847 6 TAGGACC 505 0.0 11.476252 4 TCCAACG 375 0.0 11.3966255 18 GCCGGTT 270 0.0 11.255925 11 TGGACCG 145 1.2169039E-9 11.138906 5 AAGACCG 60 0.005873345 11.084304 5 CCATGCG 60 0.0058901403 11.080051 9 TGCCGGT 285 0.0 10.996743 10 ACTGTTC 950 0.0 10.796802 8 CCTATAC 205 0.0 10.659469 3 TACACCG 90 9.510458E-5 10.556479 5 TATCGCG 100 2.4076577E-5 10.446906 7 TAGACAG 365 0.0 10.41187 5 GTCCTAC 1590 0.0 10.255918 1 >>END_MODULE