FastQCFastQC Report
Thu 26 May 2016
SRR1511705_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511705_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333151
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59790.25626288225665633No Hit
TATCAACGCAGAGTACTTTTTTTTT36320.15566930730158487No Hit
GGTATCAACGCAGAGTACTTTTTTT35200.150868932186558No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAC550.003175908212.0358683
GTCTAGG2750.011.860521
TAGGACC6900.011.6496254
GTACCGT907.853761E-611.5582546
TCTAGAC1900.011.447653
GGTATCA15300.010.6589631
TCCAACG1605.8935257E-1010.63907618
CGTGCGC803.9345963E-410.63884810
CCAACGA909.967213E-510.50772919
ACGTGTC1401.0362601E-710.13223658
CGTAGAC750.002750558810.0872043
GTATTAA3350.010.0312841
GTAGGAC14650.010.0054393
CGTAGGA1302.558385E-69.8837662
CCGTAGG1104.43057E-59.8837651
TAGGACA5550.09.8827334
TCCCGCC1151.0840442E-59.86791713
TCGAACT1651.10158E-89.74353119
GTCTTAT2550.09.6899661
GTCCTAC13300.09.6608241
Produced by FastQC (version 0.11.5)