FastQCFastQC Report
Thu 26 May 2016
SRR1511705_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511705_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2333151
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75510.323639575835426No Hit
TATCAACGCAGAGTACTTTTTTTTT47080.2017871967995213No Hit
GGTATCAACGCAGAGTACTTTTTTT46880.20092998695755226No Hit
GTCCTACAGTGGACATTTCTAAATT26140.1120373263453587No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24310.10419385629134162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT755.8716978E-815.19535411
CCGGTTT1103.8333383E-812.087213512
CTACGAC658.023558E-411.6927433
TAGGACC5900.011.5936524
TCCAACG1850.011.29384418
CGGTTTC1104.9913433E-711.22384113
GGTATCA18700.011.0187871
GGCGAGG5400.010.72820219
TGTAGGA14550.010.6430082
TGGCGAG12750.010.57715918
AGGACGT11900.010.4583735
ACCGTGC1002.407896E-510.4468078
CTAGTAC1201.5237292E-610.2920493
TCGAACT1409.737414E-810.1754619
GTAGGAC14300.010.1647143
TGCGTTT750.002655146910.13023712
GGACGTG12150.010.0833376
CTGTAGG14600.010.0621211
TTAGGAC7800.09.9875523
GTCTAGA3350.09.9665521
Produced by FastQC (version 0.11.5)