FastQCFastQC Report
Thu 26 May 2016
SRR1511704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001251
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77520.38735770775379996No Hit
GGTATCAACGCAGAGTACTTTTTTT46590.23280438085977223No Hit
TATCAACGCAGAGTACTTTTTTTTT44580.22276066320516516No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20980.10483442606649541No Hit
GGTATCAACGCAGAGTACATGGGGG20340.10163642641527725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14800.013.8886071
GTATTAA3350.011.7997351
GTACTAG958.78062E-611.44263651
CCCGAAC600.00607908711.0330113
GTATCAA31100.010.7402251
TATACTG3000.010.4025515
TAGACAC1553.949026E-910.3720455
CGCCCTA2200.010.31683716
TCTAGAC1201.6156519E-610.2449373
TTAGGGT1502.6240741E-810.0873224
CTATACT2201.8189894E-129.8867234
TAGGACC2201.8189894E-129.8867234
TAAGGCG1252.7242677E-69.8351395
GTGGTAT4700.09.6720161
CCCGTAC1304.4903863E-69.457116
CCTATAC1107.186231E-59.4568653
TAGACTA900.00116113349.4568645
ATTACAC1804.5220077E-99.4568643
GTCCTAG2001.3023964E-99.3881641
ATACTAG950.00127293329.3621581
Produced by FastQC (version 0.11.5)