##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511704_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2001251 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.14822978227119 31.0 29.0 34.0 22.0 34.0 2 26.506067954494462 31.0 25.0 34.0 10.0 34.0 3 28.144065886787814 31.0 27.0 34.0 16.0 34.0 4 32.63290661691112 35.0 32.0 37.0 25.0 37.0 5 33.06666530085432 35.0 35.0 37.0 27.0 37.0 6 33.309356497510805 37.0 35.0 37.0 28.0 37.0 7 33.33760782630465 37.0 35.0 37.0 27.0 37.0 8 33.35758682943818 37.0 35.0 37.0 27.0 37.0 9 34.922146697240876 39.0 35.0 39.0 28.0 39.0 10 34.790972746547034 39.0 35.0 39.0 27.0 39.0 11 34.80892301865183 39.0 35.0 39.0 27.0 39.0 12 34.70054830703395 39.0 35.0 39.0 27.0 39.0 13 34.67937954809267 39.0 35.0 39.0 27.0 39.0 14 35.81008679071241 40.0 36.0 41.0 27.0 41.0 15 35.71623749344785 40.0 36.0 41.0 27.0 41.0 16 35.65742777892428 40.0 36.0 41.0 27.0 41.0 17 35.63108025929781 40.0 36.0 41.0 27.0 41.0 18 35.56069878291129 40.0 36.0 41.0 27.0 41.0 19 35.57559046816216 40.0 36.0 41.0 27.0 41.0 20 35.49754478573652 40.0 36.0 41.0 26.0 41.0 21 35.414974933179295 40.0 36.0 41.0 26.0 41.0 22 35.30091078030692 40.0 36.0 41.0 25.0 41.0 23 35.17491459092338 40.0 36.0 41.0 25.0 41.0 24 35.04576137625915 40.0 35.0 41.0 24.0 41.0 25 34.92018692307961 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0284757857673767 1101 2 3.5731873922860444 1101 3 2.2048788715333245 1101 4 1.1909317861216806 1101 5 0.8086041207405188 1101 6 0.5432012286596404 1101 7 0.30372816625143884 1101 8 0.12193036714695182 1101 9 0.3047888594288892 1101 10 0.46088904961453636 1101 11 0.15647831912980337 1101 12 0.4497374446105695 1101 13 0.3633168949425638 1101 14 0.4467670313333514 1101 15 0.3650936354840084 1101 16 0.3616795113307987 1101 17 0.5738317641400457 1101 18 0.4987006968119019 1101 19 0.5374217248846378 1101 20 0.6913813292211017 1101 21 0.703235868732115 1101 22 0.5197440410362049 1101 23 0.6516398098291987 1101 24 0.6668032033566291 1101 25 0.6073329645716399 1102 1 1.7354784646649684 1102 2 4.360651030986645 1102 3 2.9289290805685155 1102 4 1.9307282981428955 1102 5 1.4461316876791344 1102 6 1.2195761931439577 1102 7 1.2702703588885313 1102 8 1.3048327768018098 1102 9 1.2712096222382172 1102 10 1.3255228659612186 1102 11 1.3160902172234614 1102 12 1.3177885466116237 1102 13 1.4067004914302288 1102 14 1.4734440482861757 1102 15 1.5730457671017604 1102 16 1.4878718237064916 1102 17 1.5340487819046515 1102 18 1.493061143836023 1102 19 1.4715563597833707 1102 20 1.4391698857282549 1102 21 1.324286862494887 1102 22 1.151162233186227 1102 23 1.0737417297058087 1102 24 1.1577456882370925 1102 25 1.0598599903188273 1103 1 1.488991411881969 1103 2 3.9397567672678804 1103 3 2.58915427635392 1103 4 1.607362654203932 1103 5 1.0545070377737673 1103 6 0.8205855684632368 1103 7 0.7961746855472924 1103 8 0.6519012258765216 1103 9 0.9109758311938236 1103 10 0.6627914223955855 1103 11 0.934459420897042 1103 12 1.0500063342568282 1103 13 0.9160561875732327 1103 14 1.1416274808433968 1103 15 0.9620253476855609 1103 16 0.9794638764975971 1103 17 1.0255769378648125 1103 18 1.0129004611454135 1103 19 1.003499423490453 1103 20 0.9979538349740551 1103 21 1.065503075423777 1103 22 1.0796269739742996 1103 23 0.971013140414847 1103 24 1.252972724705316 1103 25 0.8608920700958009 1104 1 1.693544850292266 1104 2 4.180717559515315 1104 3 2.764946578047894 1104 4 1.9425128981381334 1104 5 1.508084196789099 1104 6 1.2726719926374486 1104 7 1.1092649683311393 1104 8 1.039468100433723 1104 9 1.1564931623815937 1104 10 1.019397954282205 1104 11 1.2067023908327528 1104 12 1.143501267215619 1104 13 1.1625709592734523 1104 14 1.4228728397374937 1104 15 1.510200918299354 1104 16 1.2151540777375018 1104 17 1.2111266075808516 1104 18 1.1399502603085807 1104 19 1.3448742915241496 1104 20 1.3113557210264801 1104 21 1.34445342198552 1104 22 1.4365426287054746 1104 23 1.488776542068365 1104 24 1.291439450592108 1104 25 1.3332001511622593 1105 1 1.3886587466505453 1105 2 3.8579935039879416 1105 3 2.3125888194336532 1105 4 1.4192076517080991 1105 5 1.2069345341461784 1105 6 0.8745945314421135 1105 7 0.7842041782167684 1105 8 0.698983305818274 1105 9 0.7632771146892239 1105 10 0.45894646890849344 1105 11 0.5971390770869291 1105 12 0.8100676769552067 1105 13 0.7150378034098424 1105 14 0.6567860172386446 1105 15 0.7420658617439742 1105 16 0.7715072993516969 1105 17 0.7520621094565527 1105 18 0.7343634056377297 1105 19 0.7282214662989048 1105 20 0.803848272834621 1105 21 0.7838322480217954 1105 22 0.8283236556057503 1105 23 1.1738405044914657 1105 24 1.1510872345399221 1105 25 1.1584607069447657 1106 1 0.8338187128953614 1106 2 3.2774382598100296 1106 3 1.847331412868293 1106 4 1.0238568728740773 1106 5 0.3622885461334562 1106 6 0.3278001263938748 1106 7 0.18890881536350435 1106 8 0.13060560699182133 1106 9 0.20999910641807418 1106 10 0.2071127687408847 1106 11 0.24738972962174444 1106 12 0.207774799172725 1106 13 0.27914155303605526 1106 14 0.3160515792002627 1106 15 0.2909458502217106 1106 16 0.2790095786914861 1106 17 0.12169867575465787 1106 18 0.204125273256075 1106 19 0.16777996125941286 1106 20 0.37274487280142665 1106 21 0.19119259459453986 1106 22 0.13471648439199413 1106 23 0.06414746445258857 1106 24 0.18042843299532763 1106 25 0.003663848425489391 1107 1 1.3978928585462533 1107 2 3.8721162633578494 1107 3 2.4570755114488634 1107 4 1.567496625092133 1107 5 1.1243707101374838 1107 6 1.0533017557767153 1107 7 0.8341770190641355 1107 8 0.8222858587786206 1107 9 0.8442113895398506 1107 10 0.7560675987292456 1107 11 0.9719353834421725 1107 12 0.9529248198123526 1107 13 0.9464337838552552 1107 14 1.0299527744358201 1107 15 0.9587958997667911 1107 16 1.1115398903348606 1107 17 1.0084445103256456 1107 18 0.888627392601343 1107 19 1.1497315151853797 1107 20 1.1237830908682938 1107 21 1.2390196461749738 1107 22 1.2498337044922252 1107 23 1.3139817320851677 1107 24 1.2461442687604531 1107 25 1.2871950904320002 1108 1 1.5230260895783125 1108 2 4.044389522229391 1108 3 2.5484617405166077 1108 4 1.4659343528807511 1108 5 1.082941663558728 1108 6 1.040191120295738 1108 7 0.8126394870589877 1108 8 0.7990834113232097 1108 9 0.8723821261198239 1108 10 0.7183606519255505 1108 11 0.8753330312327776 1108 12 0.7778357561497842 1108 13 0.9428129234662563 1108 14 0.8829188994672705 1108 15 1.081448090911067 1108 16 1.1378394530887022 1108 17 1.0257937014364487 1108 18 1.0168231825547238 1108 19 0.8672630991741173 1108 20 1.0118015310150312 1108 21 0.9709558399844127 1108 22 1.1583840320758867 1108 23 1.0126323055590447 1108 24 1.0969616890621126 1108 25 1.2522440147027822 1109 1 1.2963644905450629 1109 2 3.318361377373037 1109 3 2.17891919188682 1109 4 1.4784835673975678 1109 5 1.2021694341898481 1109 6 0.9825204774859344 1109 7 0.7816762468921468 1109 8 0.7794697209166301 1109 9 0.822746605907767 1109 10 0.8898727557730552 1109 11 0.9032264550038747 1109 12 1.0315380521775381 1109 13 0.7881097698390036 1109 14 0.9721881857105927 1109 15 0.9962728677402879 1109 16 0.8317154130154165 1109 17 0.8651048931099439 1109 18 0.8399462091013206 1109 19 0.934466740073816 1109 20 1.0354644554293273 1109 21 0.9403941123112389 1109 22 0.9620044663330845 1109 23 0.9895989371420981 1109 24 1.063226843235114 1109 25 1.0433727325915285 1110 1 1.1183049908831286 1110 2 3.4973280215019535 1110 3 2.0840070891146745 1110 4 1.1907159316076203 1110 5 0.6889397745098833 1110 6 0.5724630062215681 1110 7 0.41717320429579985 1110 8 0.5173218732718254 1110 9 0.3006201691261552 1110 10 0.2567986065731418 1110 11 0.40537881019882605 1110 12 0.4177167765408498 1110 13 0.44176651989534577 1110 14 0.3918185665985803 1110 15 0.5018509610465784 1110 16 0.5264979237208465 1110 17 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-1.949498129668946 2315 19 -2.002148965222048 2315 20 -1.987796676024061 2315 21 -2.194447455682166 2315 22 -2.139367052363319 2315 23 -1.9885007505907168 2315 24 -1.9606433677433799 2315 25 -1.8973547548464538 2316 1 -0.728695289464504 2316 2 1.5969686188534205 2316 3 0.36248046061146155 2316 4 -0.6533444667271766 2316 5 -0.8404726108264313 2316 6 -0.9898962547459078 2316 7 -1.5133616266364918 2316 8 -1.515943031123605 2316 9 -1.6650451591066613 2316 10 -1.9315369725557332 2316 11 -1.627949398500391 2316 12 -1.775427668807943 2316 13 -1.7817719043098066 2316 14 -2.149873723238663 2316 15 -2.2413777393160643 2316 16 -2.2939537374142276 2316 17 -2.0457295586880875 2316 18 -2.3480102401975174 2316 19 -2.262604039457507 2316 20 -2.16547235517217 2316 21 -2.448249228581375 2316 22 -2.180151239379569 2316 23 -2.425685075754963 2316 24 -2.25633317589449 2316 25 -2.253005370183807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 77679.0 3 5.0 4 698.0 5 3417.0 6 4068.0 7 3592.0 8 3389.0 9 3331.0 10 3183.0 11 3086.0 12 3056.0 13 2966.0 14 3070.0 15 3186.0 16 3349.0 17 3758.0 18 4089.0 19 5050.0 20 6464.0 21 8477.0 22 10175.0 23 12179.0 24 14258.0 25 16268.0 26 18504.0 27 21110.0 28 24037.0 29 29075.0 30 36022.0 31 45477.0 32 58471.0 33 76819.0 34 104752.0 35 148364.0 36 232313.0 37 295111.0 38 712403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59665479242734 17.76233965654483 12.749575140749462 24.89143041027837 2 17.690821947206526 20.635906936525746 37.60379881605515 24.06947230021257 3 19.00151455264732 24.044809971362913 29.07511351649543 27.878561959494334 4 13.166564314021578 16.229348542486672 36.379694501089574 34.22439264240218 5 14.440767300053817 36.73552193103214 34.19171308346629 14.631997685447754 6 33.03043946011769 35.51993228235739 17.484913186801656 13.964715070723264 7 29.117087261917668 31.394862513497806 21.14087638182317 18.347173842761354 8 27.04338436308089 34.158084118383954 20.026073690906337 18.77245782762882 9 27.31691327074915 15.191047999476329 19.43984037984241 38.05219834993211 10 16.64169062251562 27.696375916864003 32.25347545110534 23.408458009515048 11 35.26039462316321 22.465647737340294 22.91721528184121 19.35674235765529 12 24.126558837447178 24.25203035501294 29.687530449703708 21.933880357836173 13 29.334751113178708 21.09485516809236 25.282735648851645 24.287658069877292 14 22.897477627743847 20.58812212960793 26.557938009774883 29.95646223287334 15 24.420674867870147 27.81235337296521 23.452180660996547 24.314791098168094 16 23.95276754390129 27.244058841194835 24.941349186084103 23.861824428819773 17 23.205435000407245 26.994165149698862 26.285358508253086 23.515041341640806 18 23.28418574181849 26.379599560474926 27.33380270640714 23.002411991299443 19 24.24308594973844 26.09692637255397 27.03698836377846 22.62299931392914 20 24.748219988397256 25.47376615926738 26.836014073197216 22.94199977913815 21 24.803947532792005 25.506458463460334 26.588032479700185 23.10156152404747 22 24.788442713509834 25.36683671200376 26.69327029213519 23.151450282351213 23 24.320382164013722 25.425399815613858 26.657538969686218 23.596679050686202 24 24.002572394825627 25.537314715617455 26.863037464622074 23.59707542493484 25 24.094279028467692 25.584796709658107 26.770904799048196 23.550019462826004 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 419.0 1 419.0 2 555.0 3 691.0 4 691.0 5 691.0 6 1786.5 7 2882.0 8 2882.0 9 2882.0 10 3550.5 11 4219.0 12 4219.0 13 4219.0 14 6241.0 15 8263.0 16 8263.0 17 8263.0 18 14011.0 19 19759.0 20 19759.0 21 19759.0 22 29704.0 23 39649.0 24 39649.0 25 39649.0 26 55764.0 27 71879.0 28 71879.0 29 71879.0 30 90644.0 31 109409.0 32 109409.0 33 109409.0 34 128331.0 35 147253.0 36 147253.0 37 147253.0 38 165766.5 39 184280.0 40 184280.0 41 184280.0 42 203262.0 43 222244.0 44 222244.0 45 222244.0 46 239447.5 47 256651.0 48 256651.0 49 256651.0 50 259550.0 51 262449.0 52 262449.0 53 262449.0 54 247647.5 55 232846.0 56 232846.0 57 232846.0 58 206435.0 59 180024.0 60 180024.0 61 180024.0 62 153079.5 63 126135.0 64 126135.0 65 126135.0 66 99989.5 67 73844.0 68 73844.0 69 73844.0 70 54730.5 71 35617.0 72 35617.0 73 35617.0 74 25019.5 75 14422.0 76 14422.0 77 14422.0 78 10146.5 79 5871.0 80 5871.0 81 5871.0 82 3775.0 83 1679.0 84 1679.0 85 1679.0 86 1107.0 87 535.0 88 535.0 89 535.0 90 352.5 91 170.0 92 170.0 93 170.0 94 105.5 95 41.0 96 41.0 97 41.0 98 30.5 99 20.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.303708030626843 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 4.99687445502838E-5 22 9.993748910056758E-4 23 7.994999128045408E-4 24 1.4990623365085138E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2001251.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.469002981249496 #Duplication Level Percentage of deduplicated Percentage of total 1 65.93499723249705 32.61738574663073 2 16.383404385038123 16.20941360732934 3 7.150120855499566 10.611280497510068 4 3.789028232343173 7.497577956872917 5 2.1279981073249874 5.263497235767654 6 1.3078921561487706 3.882007258300582 7 0.8218717203846553 2.8460022210139218 8 0.5622036140293356 2.2249321806789157 9 0.39845246482459806 1.7739941553264824 >10 1.4543572156154092 11.328702673194627 >50 0.04389354472765214 1.497764948325013 >100 0.023306348310502396 2.2743738642241786 >500 0.0014432385661682912 0.5418923985571698 >1k 9.277962211081867E-4 1.035847769573991 >5k 1.0308846901202075E-4 0.3953274866944455 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7752 0.38735770775379996 No Hit GGTATCAACGCAGAGTACTTTTTTT 4659 0.23280438085977223 No Hit TATCAACGCAGAGTACTTTTTTTTT 4458 0.22276066320516516 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2098 0.10483442606649541 No Hit GGTATCAACGCAGAGTACATGGGGG 2034 0.10163642641527725 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1480 0.0 13.888607 1 GTATTAA 335 0.0 11.799735 1 GTACTAG 95 8.78062E-6 11.4426365 1 CCCGAAC 60 0.006079087 11.03301 13 GTATCAA 3110 0.0 10.740225 1 TATACTG 300 0.0 10.402551 5 TAGACAC 155 3.949026E-9 10.372045 5 CGCCCTA 220 0.0 10.316837 16 TCTAGAC 120 1.6156519E-6 10.244937 3 TTAGGGT 150 2.6240741E-8 10.087322 4 CTATACT 220 1.8189894E-12 9.886723 4 TAGGACC 220 1.8189894E-12 9.886723 4 TAAGGCG 125 2.7242677E-6 9.835139 5 GTGGTAT 470 0.0 9.672016 1 CCCGTAC 130 4.4903863E-6 9.4571 16 CCTATAC 110 7.186231E-5 9.456865 3 TAGACTA 90 0.0011611334 9.456864 5 ATTACAC 180 4.5220077E-9 9.456864 3 GTCCTAG 200 1.3023964E-9 9.388164 1 ATACTAG 95 0.0012729332 9.362158 1 >>END_MODULE