FastQCFastQC Report
Thu 26 May 2016
SRR1511704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001251
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT96430.48184860369838667No Hit
TATCAACGCAGAGTACTTTTTTTTT60860.3041097793330272No Hit
GGTATCAACGCAGAGTACTTTTTTT60430.30196112331736497No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29970.14975632741720055No Hit
GGTATCAACGCAGAGTACATGGGGG22800.11392873757464705No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22770.11377883134099621No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGT653.236255E-614.6739061
GGTATCA17800.013.6104611
AACGCGG600.00589025111.0798936
ACGACTG700.00149061410.8581145
TATACTG2850.010.6676215
GTATTAT1658.6765795E-1010.4051341
GTGGTAT5700.010.2073761
TAGGACT2250.010.134244
CGTGCGC750.002654968310.13018810
GTATCAA36000.010.0944261
CACGACT856.5892894E-410.0597234
GTATAGG1909.276846E-1110.0400421
GTCCTAG2002.3646862E-1110.0149421
CCCTTAG1159.78985E-69.9527371
ACGGTGT1151.0304057E-59.9099678
TTAGGAC2400.09.8967183
CAGGACG1252.5674417E-69.8808834
CCCGTAC1451.6246668E-79.82453616
TAGTACT1856.475602E-109.7576294
GGTTTAG1401.0025287E-69.538041
Produced by FastQC (version 0.11.5)