FastQCFastQC Report
Thu 26 May 2016
SRR1511703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679541
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41440.24673407794153285No Hit
GTCCTACAGTGGACATTTCTAAATT35070.20880704906876343No Hit
CTGTAGGACGTGGAATATGGCAAGA29760.17719126832866836No Hit
TATCAACGCAGAGTACTTTTTTTTT23940.14253894367568282No Hit
GGTATCAACGCAGAGTACTTTTTTT23270.13854975853521884No Hit
GTCCTAAAGTGTGTATTTCTCATTT22920.13646585584990184No Hit
CTTTAGGACGTGAAATATGGCGAGG18160.10812477932959064No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT250.004782582619.7785721
GGACCGC453.6821933E-516.8110356
CGGTAGG609.647065E-716.4821451
TGGACCG400.005443640514.1843115
GTGCGCC801.3634599E-714.18431111
TAGGACC6000.013.5538984
CTTTGCG500.001552758313.238699
GCGTGCG550.00317664412.0351739
AGGACCG951.0995918E-611.9446825
CTAGGAC2550.011.8666123
GTAGGAC17300.011.7519343
AAGGCGT1052.8881914E-711.7076856
TGTAGGA17500.011.6411042
GGTCTAC1801.8189894E-1211.5375011
TCCAACG1657.2759576E-1211.462069518
TGCGCCA1002.039953E-611.34744812
GGCGAGG4950.011.27103519
GGTATCA8750.011.1890221
CCAACGA1105.274196E-711.17551819
GTCCTAC16750.011.0996171
Produced by FastQC (version 0.11.5)