FastQCFastQC Report
Thu 26 May 2016
SRR1511703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679541
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51070.304071171826112No Hit
TATCAACGCAGAGTACTTTTTTTTT33280.19814937533528507No Hit
GGTATCAACGCAGAGTACTTTTTTT33190.19761351464477497No Hit
GTCCTACAGTGGACATTTCTAAATT32850.1955891520361813No Hit
CTGTAGGACGTGGAATATGGCAAGA31680.1886229630595502No Hit
GTCCTAAAGTGTGTATTTCTCATTT22560.1343224130878615No Hit
CTTTAGGACGTGAAATATGGCGAGG18900.112530745007118No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18900.112530745007118No Hit
GTCCTACAGTGTGCATTTCTCATTT17070.10163491096674628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA903.651803E-813.71867519
TAGGACC6000.012.1921444
AACCGCT550.00307525612.0874237
AGGACCG951.0394342E-612.0004715
AATCCCG2400.011.47619919
GGTATCA9700.011.4047431
TAAGACG600.00587427711.0837684
TGTAGGA18450.010.9679112
GTAGGAC17350.010.8966813
TACACCG700.001491023510.8575685
AGGACCT11650.010.7643715
GAGAGCG803.7810768E-410.6844197
ACCGGGG909.5430594E-510.55251215
GATATAC4750.010.4402051
CTTATAC1856.002665E-1110.2706723
AACAGTG5650.010.2536797
TTATACT2600.010.2311714
GGACCTG12200.010.197886
GGCGAGG5050.010.1557919
GTCCCGC750.002654102610.13041112
Produced by FastQC (version 0.11.5)