FastQCFastQC Report
Thu 26 May 2016
SRR1511702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288134
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64070.2800098245994334No Hit
GTCCTACAGTGGACATTTCTAAATT48000.2097779238453692No Hit
CTGTAGGACGTGGAATATGGCAAGA41110.17966605102673183No Hit
GGTATCAACGCAGAGTACTTTTTTT38640.16887122869552218No Hit
GTCCTAAAGTGTGTATTTCTCATTT36100.1577704802253714No Hit
TATCAACGCAGAGTACTTTTTTTTT35690.1559786271258589No Hit
CTTTAGGACGTGAAATATGGCGAGG30250.13220379575671704No Hit
GTCCTACAGTGTGCATTTCTCATTT23110.10099932958471838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1301.8189894E-1213.82129819
TCCAACG2500.013.61761618
CGAAATC1500.013.23905913
GACCCGT500.001552731813.23905856
CGTTAGG550.002276037112.5826771
CGTAGGA655.741705E-412.1678622
ACCGTGC803.005709E-511.8205878
GGCGAGG7900.011.73106819
CCAACGT1302.8121576E-911.63898819
GCCGGTT2450.011.57935111
TAGGACC8300.011.5072714
ACGAAAT1657.2759576E-1211.46238812
GGTATCA12550.011.18619251
GTCCTAC22100.011.1836971
CGAGACA803.9356254E-410.63852914
ATTTAGA5800.010.5682081
TCCTACA24900.010.4025452
CGTTTCC2100.010.35708714
CCTACAG26100.010.3260313
TGGCGAG21750.010.30451718
Produced by FastQC (version 0.11.5)