##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511702_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2288134 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.339342888135047 31.0 30.0 34.0 23.0 34.0 2 26.67453960301276 31.0 26.0 34.0 10.0 34.0 3 28.347164545433092 31.0 28.0 34.0 16.0 34.0 4 32.82891474013323 35.0 32.0 37.0 26.0 37.0 5 33.291779677239184 35.0 35.0 37.0 28.0 37.0 6 33.54450089024506 37.0 35.0 37.0 28.0 37.0 7 33.59157680450533 37.0 35.0 37.0 28.0 37.0 8 33.609323142787964 37.0 35.0 37.0 28.0 37.0 9 35.202369703872236 39.0 35.0 39.0 29.0 39.0 10 35.07649377178085 39.0 35.0 39.0 28.0 39.0 11 35.092006849249216 39.0 35.0 39.0 28.0 39.0 12 34.995904086036916 39.0 35.0 39.0 28.0 39.0 13 34.99180292762574 39.0 35.0 39.0 28.0 39.0 14 36.13123969138171 40.0 36.0 41.0 27.0 41.0 15 36.07488460029002 40.0 36.0 41.0 27.0 41.0 16 36.01492001779616 40.0 36.0 41.0 27.0 41.0 17 35.996025582417815 40.0 36.0 41.0 27.0 41.0 18 35.936130925898574 40.0 36.0 41.0 27.0 41.0 19 35.9711485428738 40.0 36.0 41.0 27.0 41.0 20 35.8989289962913 40.0 36.0 41.0 27.0 41.0 21 35.817282554256 40.0 36.0 41.0 27.0 41.0 22 35.71176469559912 40.0 36.0 41.0 27.0 41.0 23 35.60879214241823 40.0 36.0 41.0 27.0 41.0 24 35.497224812882465 40.0 36.0 41.0 27.0 41.0 25 35.392992281046475 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2215534848518352 1101 2 3.806887646605041 1101 3 2.3206588009030753 1101 4 1.3713807974474577 1101 5 0.9104509170649422 1101 6 0.6710332032014819 1101 7 0.5901917803213266 1101 8 0.4042976993988532 1101 9 0.31926289096637106 1101 10 0.4651553494166478 1101 11 0.44683705827887366 1101 12 0.49468457450315384 1101 13 0.4710279332424676 1101 14 0.6599186992357815 1101 15 0.5328915624716402 1101 16 0.5324717593504289 1101 17 0.6522017888641685 1101 18 0.5412288877884208 1101 19 0.5450493530188965 1101 20 0.6101028174570118 1101 21 0.6680922585542817 1101 22 0.5594034610831073 1101 23 0.6482330562972507 1101 24 0.6913336378242292 1101 25 0.5800584736617296 1102 1 1.396501892956877 1102 2 4.03562535516491 1102 3 2.6122377759635818 1102 4 1.6663643991171 1102 5 1.1233624830031843 1102 6 0.8942200330914574 1102 7 0.8571191833636362 1102 8 0.8682644876645469 1102 9 0.9470675608367003 1102 10 0.9086965703176517 1102 11 0.9831092296399646 1102 12 0.9843428110834154 1102 13 0.9861627737022047 1102 14 1.024156016106481 1102 15 0.9795158740925345 1102 16 1.1628823314504686 1102 17 1.190177369674906 1102 18 0.9657271456485148 1102 19 0.9461186060033384 1102 20 1.0006593083897002 1102 21 1.0034154605840513 1102 22 0.9184063198294723 1102 23 0.9995840916428165 1102 24 0.818580735232068 1102 25 0.7278129839333332 1103 1 1.4268164068263083 1103 2 4.082311635168647 1103 3 2.7018599857328915 1103 4 1.5587271057576189 1103 5 1.2277691795568373 1103 6 0.9724921914925062 1103 7 0.8332226670658898 1103 8 0.6374026682940368 1103 9 0.9101823455569829 1103 10 0.7319719976082126 1103 11 0.8366938004814699 1103 12 0.928633462059409 1103 13 0.8807917547426456 1103 14 1.0512798603205482 1103 15 0.8924263532183403 1103 16 0.9282783340655811 1103 17 1.0132737085186534 1103 18 1.0245454420900941 1103 19 1.0878567212550436 1103 20 1.0860889338683606 1103 21 1.1157851719878025 1103 22 1.1430978767548439 1103 23 0.9323533080433037 1103 24 0.973093437202742 1103 25 0.8510818734448691 1104 1 1.5247326196739692 1104 2 4.146303595554457 1104 3 2.6631663107533825 1104 4 1.9607181454054938 1104 5 1.5059685754629228 1104 6 1.192619073671736 1104 7 1.0096654746428868 1104 8 1.0047917025297721 1104 9 1.1488798382303784 1104 10 1.0586293820387525 1104 11 1.160412234328696 1104 12 1.1672647065153328 1104 13 0.9993001592834574 1104 14 1.301760103754674 1104 15 1.285316010636457 1104 16 1.0962945929501444 1104 17 0.9488337305265375 1104 18 1.0931071820602227 1104 19 1.0951375126405267 1104 20 1.2499540120749444 1104 21 1.1356151723246555 1104 22 1.236726594795698 1104 23 1.4138720765001693 1104 24 1.2916950383884682 1104 25 1.1982104062152814 1105 1 1.4252138682401352 1105 2 3.8279724356530416 1105 3 2.4470496228294394 1105 4 1.5383755833347479 1105 5 1.0249966936149306 1105 6 0.7564076018542849 1105 7 0.7002281108979886 1105 8 0.6557659305566759 1105 9 0.6573501512609496 1105 10 0.6367393592838582 1105 11 0.6652860849287094 1105 12 0.7183528608271175 1105 13 0.6795164067242325 1105 14 0.7114510803332195 1105 15 0.8495859174875164 1105 16 0.7414482589956393 1105 17 0.95482971383354 1105 18 0.9035450328215617 1105 19 0.8790286308571567 1105 20 0.8252563807615658 1105 21 0.7665699023471149 1105 22 0.8095173377537961 1105 23 0.9223460757637838 1105 24 0.8795891454895894 1105 25 0.9776353278394652 1106 1 1.0890023841039493 1106 2 3.708535729009693 1106 3 2.122786182600617 1106 4 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0.8335991679629657 1107 17 0.7308478252101409 1107 18 0.7879715058958823 1107 19 0.8490669554502475 1107 20 0.8998841145912522 1107 21 0.9924789132449661 1107 22 1.0406789877155447 1107 23 0.9719079310296266 1107 24 1.0284546573642857 1107 25 1.1245772030780117 1108 1 1.5567197564886932 1108 2 4.10013766456094 1108 3 2.507696077352115 1108 4 1.6286701747368326 1108 5 1.2087515706597074 1108 6 1.0053691940080611 1108 7 0.8931035091154058 1108 8 0.8226270824341171 1108 9 0.7784254277759644 1108 10 0.7383694555719345 1108 11 0.7515673606562387 1108 12 0.8474418825545769 1108 13 1.0255607207164275 1108 14 0.823216839004516 1108 15 1.1303662446522083 1108 16 1.053531875704664 1108 17 0.942711018196924 1108 18 0.9687689294461137 1108 19 0.9417425944180238 1108 20 1.0780709277780645 1108 21 0.9846849469116776 1108 22 1.0859020605228835 1108 23 1.0884025599961689 1108 24 1.184675589989709 1108 25 1.2370318271056675 1109 1 1.1403399565133867 1109 2 3.1993523824273424 1109 3 2.0052809643752063 1109 4 1.1800814600807001 1109 5 0.8723989345317946 1109 6 0.5800722437672903 1109 7 0.4051148685424977 1109 8 0.4610762736922922 1109 9 0.4476487886847167 1109 10 0.29245267572257205 1109 11 0.5154419130701129 1109 12 0.5165096687010191 1109 13 0.4366139371629032 1109 14 0.5912375823061566 1109 15 0.4739494030668254 1109 16 0.44995612561206144 1109 17 0.4957570732654517 1109 18 0.36075312502581625 1109 19 0.5111239995297652 1109 20 0.552541850114153 1109 21 0.6203239146854713 1109 22 0.5919250263211282 1109 23 0.5503435403641106 1109 24 0.5914048158200629 1109 25 0.6645512739509556 1110 1 1.0611585303267006 1110 2 3.48491654992781 1110 3 2.208914466972722 1110 4 1.3564790680165402 1110 5 0.7352934735799721 1110 6 0.6962252318773068 1110 7 0.455034054071092 1110 8 0.38750251377737754 1110 9 0.4207507717970884 1110 10 0.4915262093422328 1110 11 0.3241325713296703 1110 12 0.4209186145996924 1110 13 0.370231542012732 1110 14 0.29985805037090785 1110 15 0.30545831917334 1110 16 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-1.7812703404826635 2315 15 -1.7278189759243148 2315 16 -2.022397412398533 2315 17 -1.8879276185286642 2315 18 -1.896941374742454 2315 19 -1.9789740802116924 2315 20 -2.021492697002948 2315 21 -1.9024364099922906 2315 22 -1.9447509096953013 2315 23 -1.8814994944711074 2315 24 -1.8319711690483516 2315 25 -1.985331606562113 2316 1 -0.49225687681226304 2316 2 1.8937532819603433 2316 3 0.5244544395158819 2316 4 -0.40884433412902865 2316 5 -0.7218468448035722 2316 6 -0.7165267916717042 2316 7 -1.102057151991204 2316 8 -1.1583905850700376 2316 9 -1.3646336752831374 2316 10 -1.4017872321523228 2316 11 -1.2692746753209363 2316 12 -1.5374152763566329 2316 13 -1.4048206437189918 2316 14 -1.6601174308162427 2316 15 -1.5620555920939552 2316 16 -1.7161408620761804 2316 17 -1.5638107872437956 2316 18 -1.8396275097611365 2316 19 -1.6975881113991704 2316 20 -1.622303613677559 2316 21 -1.9901877041771954 2316 22 -1.631916512430827 2316 23 -1.8126083647985425 2316 24 -1.8219418977088537 2316 25 -1.83896749862339 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79447.0 3 2.0 4 728.0 5 3423.0 6 4255.0 7 3799.0 8 3614.0 9 3338.0 10 3252.0 11 3113.0 12 3026.0 13 3105.0 14 3059.0 15 3157.0 16 3432.0 17 3677.0 18 4212.0 19 5295.0 20 6818.0 21 8788.0 22 10591.0 23 12725.0 24 14905.0 25 17012.0 26 19310.0 27 21826.0 28 25662.0 29 31136.0 30 39303.0 31 50032.0 32 65250.0 33 86423.0 34 118735.0 35 169577.0 36 270256.0 37 346843.0 38 839008.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.91527506693227 18.93639096311667 13.081183182453476 25.06715078749758 2 16.037212133008566 20.858788627280582 40.97529014580281 22.128709093908046 3 19.15049555664135 24.1105634547627 29.519381294976604 27.219559693619345 4 11.764477080450709 16.909630292631466 39.23105027939797 32.09484234751986 5 12.780763714013252 37.89183675431596 36.394983860210985 12.932415671459802 6 31.19380246086986 37.95289087090179 18.319949793150226 12.53335687507812 7 27.78657194027972 31.576472356951125 22.231128072044733 18.405827630724424 8 24.0270893225659 38.576936490607636 20.651456601754965 16.744517585071502 9 25.63333266320941 16.011999297243957 20.683622550077924 37.67104548946871 10 14.124435019976978 29.86721931495271 35.09833777217593 20.910007892894384 11 34.046738521432744 22.87440333476973 24.76660020785496 18.312257935942565 12 22.809984030655546 24.917334386884686 32.54468488296577 19.727996699494 13 28.774363739186605 22.213690282125086 26.110839662362434 22.90110631632588 14 20.05944581916968 22.807886251417095 28.268099682973112 28.864568246440115 15 22.309095533740596 31.72843024053661 23.736022453230447 22.226451772492346 16 21.167379183212173 28.97815425145555 28.711168139628185 21.14329842570409 17 20.971455343087424 29.127052873651632 28.543083578147083 21.35840820511386 18 20.651806231628044 28.201670007088747 30.78700810354638 20.35951565773683 19 22.647231324738847 27.388780552187942 28.24165892382177 21.72232919925144 20 22.467128236370772 28.035989150985042 29.208516634078247 20.28836597856594 21 22.101167417207428 27.42093099686731 28.04572121839338 22.43218036753189 22 21.87414776899033 28.866899872034235 28.483876663380116 20.775075695595323 23 21.50864662952113 28.308735960617398 28.68874275222814 21.49387465763333 24 22.06745758771121 27.365748684299085 29.625712480125728 20.94108124786398 25 21.949195283143382 27.96650021371126 28.98873055511609 21.095573948029266 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 190.0 1 190.0 2 386.5 3 583.0 4 583.0 5 583.0 6 1599.0 7 2615.0 8 2615.0 9 2615.0 10 4101.5 11 5588.0 12 5588.0 13 5588.0 14 8970.0 15 12352.0 16 12352.0 17 12352.0 18 21563.5 19 30775.0 20 30775.0 21 30775.0 22 48263.0 23 65751.0 24 65751.0 25 65751.0 26 93099.5 27 120448.0 28 120448.0 29 120448.0 30 155446.0 31 190444.0 32 190444.0 33 190444.0 34 213967.5 35 237491.0 36 237491.0 37 237491.0 38 252443.5 39 267396.0 40 267396.0 41 267396.0 42 279952.5 43 292509.0 44 292509.0 45 292509.0 46 294910.5 47 297312.0 48 297312.0 49 297312.0 50 282670.5 51 268029.0 52 268029.0 53 268029.0 54 234804.0 55 201579.0 56 201579.0 57 201579.0 58 169939.5 59 138300.0 60 138300.0 61 138300.0 62 110943.5 63 83587.0 64 83587.0 65 83587.0 66 63272.0 67 42957.0 68 42957.0 69 42957.0 70 31022.5 71 19088.0 72 19088.0 73 19088.0 74 13268.5 75 7449.0 76 7449.0 77 7449.0 78 4996.5 79 2544.0 80 2544.0 81 2544.0 82 1678.0 83 812.0 84 812.0 85 812.0 86 529.0 87 246.0 88 246.0 89 246.0 90 155.5 91 65.0 92 65.0 93 65.0 94 40.5 95 16.0 96 16.0 97 16.0 98 12.0 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.348215620239024 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.6222240480671153E-4 22 9.614821509579422E-4 23 7.429634802856826E-4 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2288134.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.63575944554469 #Duplication Level Percentage of deduplicated Percentage of total 1 85.50201587307662 64.67009904685167 2 10.69341728672126 16.176094750985566 3 2.2315893100347672 5.063638567051161 4 0.6858953126300936 2.075128514836657 5 0.2999730461743016 1.134434458029337 6 0.1474101401169666 0.6689686738632553 7 0.08835737888003747 0.46780842179465554 8 0.056998861544476566 0.344892174435835 9 0.045132108811900724 0.30722411928303633 >10 0.21540865137247064 2.9600697627579446 >50 0.016844340304175087 0.8598040036038725 >100 0.013803729661569723 2.0158648893478395 >500 0.0016937936938502404 0.861350185735624 >1k 0.0014017602983553178 2.111584651138493 >5k 5.840667909813824E-5 0.2830377802850336 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6407 0.2800098245994334 No Hit GTCCTACAGTGGACATTTCTAAATT 4800 0.2097779238453692 No Hit CTGTAGGACGTGGAATATGGCAAGA 4111 0.17966605102673183 No Hit GGTATCAACGCAGAGTACTTTTTTT 3864 0.16887122869552218 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3610 0.1577704802253714 No Hit TATCAACGCAGAGTACTTTTTTTTT 3569 0.1559786271258589 No Hit CTTTAGGACGTGAAATATGGCGAGG 3025 0.13220379575671704 No Hit GTCCTACAGTGTGCATTTCTCATTT 2311 0.10099932958471838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.370373413445192E-5 2 0.0 0.0 0.0 0.0 4.370373413445192E-5 3 0.0 0.0 0.0 0.0 4.370373413445192E-5 4 0.0 0.0 0.0 0.0 4.370373413445192E-5 5 0.0 0.0 0.0 0.0 4.370373413445192E-5 6 0.0 0.0 0.0 0.0 4.370373413445192E-5 7 0.0 0.0 0.0 4.370373413445192E-5 4.370373413445192E-5 8 0.0 0.0 0.0 4.370373413445192E-5 4.370373413445192E-5 9 0.0 0.0 0.0 8.740746826890384E-5 4.370373413445192E-5 10 0.0 0.0 0.0 1.3111120240335574E-4 4.370373413445192E-5 11 0.0 0.0 0.0 1.3111120240335574E-4 4.370373413445192E-5 12 0.0 0.0 0.0 1.3111120240335574E-4 1.3111120240335574E-4 13 0.0 0.0 0.0 1.7481493653780767E-4 1.7481493653780767E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 130 1.8189894E-12 13.821298 19 TCCAACG 250 0.0 13.617616 18 CGAAATC 150 0.0 13.239059 13 GACCCGT 50 0.0015527318 13.2390585 6 CGTTAGG 55 0.0022760371 12.582677 1 CGTAGGA 65 5.741705E-4 12.167862 2 ACCGTGC 80 3.005709E-5 11.820587 8 GGCGAGG 790 0.0 11.731068 19 CCAACGT 130 2.8121576E-9 11.638988 19 GCCGGTT 245 0.0 11.579351 11 TAGGACC 830 0.0 11.507271 4 ACGAAAT 165 7.2759576E-12 11.462388 12 GGTATCA 1255 0.0 11.1861925 1 GTCCTAC 2210 0.0 11.183697 1 CGAGACA 80 3.9356254E-4 10.638529 14 ATTTAGA 580 0.0 10.568208 1 TCCTACA 2490 0.0 10.402545 2 CGTTTCC 210 0.0 10.357087 14 CCTACAG 2610 0.0 10.326031 3 TGGCGAG 2175 0.0 10.304517 18 >>END_MODULE