FastQCFastQC Report
Thu 26 May 2016
SRR1511702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288134
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT80740.35286394940156474No Hit
GTCCTACAGTGGACATTTCTAAATT53380.23329053280970433No Hit
GGTATCAACGCAGAGTACTTTTTTT50090.21891200427946964No Hit
TATCAACGCAGAGTACTTTTTTTTT48130.21034607238911707No Hit
CTGTAGGACGTGGAATATGGCAAGA47400.20715569979730206No Hit
GTCCTAAAGTGTGTATTTCTCATTT39160.17114382287051372No Hit
CTTTAGGACGTGAAATATGGCGAGG33800.14771862137444747No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25520.11153192951112129No Hit
GTCCTACAGTGTGCATTTCTCATTT24170.10563192540297028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTC1950.013.63680613
TCCAACG2950.013.52123818
TAGGACC7750.013.3621214
CCAACGA1850.013.3472219
GCCGGTT1700.012.84896511
CCGGTTT1850.012.32051112
TGCCGGT1850.011.80715710
GGACCGA658.0468215E-411.688696
CGTGCGC1001.9379004E-611.39647210
ATCCCGT1803.6379788E-1211.07990510
CGAACCG600.00589064811.07990412
TGTAGGA26100.010.9202182
GACGTGA10550.010.8923647
GGTATCA12900.010.8699771
AGGACCT14850.010.81211955
AGGACGT22600.010.761735
ATAGGAC4150.010.7597053
GTCCTAG3550.010.7481231
GGACGTG22450.010.7027016
GGCGAGG9150.010.690680519
Produced by FastQC (version 0.11.5)