FastQCFastQC Report
Thu 26 May 2016
SRR1511701_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511701_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097244
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52310.24942257553246067No Hit
GTCCTACAGTGGACATTTCTAAATT48450.23101746863979583No Hit
CTGTAGGACGTGGAATATGGCAAGA46730.2228162292990229No Hit
GTCCTAAAGTGTGTATTTCTCATTT40400.19263376126001552No Hit
CTTTAGGACGTGAAATATGGCGAGG34590.16493073767286973No Hit
GGTATCAACGCAGAGTACTTTTTTT30210.1440461863283433No Hit
TATCAACGCAGAGTACTTTTTTTTT30070.14337864359130364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1253.6379788E-1214.23487951
GTACCGT400.005442613714.1849496
GGACCGT500.001552456213.2392856
CGTTTAT759.822783E-613.1804441
AGGACCG655.6934434E-513.09385
CGTAGGA1008.592906E-812.8509332
TAGGACC8300.012.53288654
CCAACGA1552.7284841E-1111.59200219
AGCACCG1002.0396437E-611.3479595
CCCCGTA752.1645067E-411.34795915
TCCAACG1950.011.15397718
GGCGAGG10050.011.10331119
CCCGTAC600.006080360611.03273916
TAGGTCG700.001549804610.807585
AAGCGTG700.001549804610.807587
AGGACGT26100.010.7972285
GGACGTG25700.010.6709086
CCACCTT6650.010.66537613
TTAGGAC13500.010.6474693
GGTATCA9300.010.6293911
Produced by FastQC (version 0.11.5)