FastQCFastQC Report
Thu 26 May 2016
SRR1511701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097244
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65520.31241000093455984No Hit
GTCCTACAGTGGACATTTCTAAATT54170.2582913576102733No Hit
CTGTAGGACGTGGAATATGGCAAGA51480.245465000734297No Hit
GTCCTAAAGTGTGTATTTCTCATTT44200.2107527784082348No Hit
GGTATCAACGCAGAGTACTTTTTTT40590.1935397121174265No Hit
TATCAACGCAGAGTACTTTTTTTTT39940.19044040655259953No Hit
CTTTAGGACGTGAAATATGGCGAGG38390.183049754821089No Hit
CTGAAGGACCTGGAATATGGCGAGA22800.1087141028893157No Hit
GTCCTACAGTGTGCATTTCTCATTT22400.10680683792634524No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21090.100560545172617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT400.005147415714.3046891
GGTATCA11150.012.8293181
TAGGACC7600.012.7500964
GGCGAGG11400.011.08077419
TATGTCG951.3649196E-510.99693716
GCCCTAT1053.3119231E-610.8988111
GACCGTG1158.8518937E-710.7361397
CGGACAG803.7715957E-410.687585
GGACGTG26350.010.5245826
GTCCTAC26500.010.4361271
GTATCAA25000.010.4138141
AGGACGT27300.010.3700425
CCACCTT7250.010.34884113
TGTAGGA26500.010.3246072
GACGTGG13550.010.3034027
CTGTAGG26450.010.275581
GTAGGAC26850.010.1546393
AATCCCG3750.010.13099419
GGACCGT750.002654358710.130516
GTCCTAG2650.010.076261
Produced by FastQC (version 0.11.5)