##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511701_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2097244 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36503525579284 33.0 31.0 34.0 28.0 34.0 2 31.550444774189366 33.0 31.0 34.0 28.0 34.0 3 31.667203243876248 34.0 31.0 34.0 28.0 34.0 4 35.202490983404886 37.0 35.0 37.0 32.0 37.0 5 34.90132192534583 37.0 35.0 37.0 32.0 37.0 6 34.86699020237989 37.0 35.0 37.0 32.0 37.0 7 34.72053132587338 37.0 35.0 37.0 32.0 37.0 8 34.64654374979735 37.0 35.0 37.0 32.0 37.0 9 36.22217443463899 39.0 37.0 39.0 32.0 39.0 10 36.04743797097524 39.0 37.0 39.0 31.0 39.0 11 36.04796771381871 39.0 37.0 39.0 31.0 39.0 12 35.906991747264506 39.0 37.0 39.0 30.0 39.0 13 35.895201988895906 39.0 37.0 39.0 30.0 39.0 14 37.01183410227899 40.0 37.0 41.0 31.0 41.0 15 36.957104657350314 40.0 37.0 41.0 31.0 41.0 16 36.81187405947996 40.0 37.0 41.0 30.0 41.0 17 36.796630244263426 40.0 37.0 41.0 31.0 41.0 18 36.740031202854794 40.0 37.0 41.0 30.0 41.0 19 36.76490098433945 40.0 37.0 41.0 30.0 41.0 20 36.873424360732464 40.0 37.0 41.0 31.0 41.0 21 36.823504084407915 40.0 37.0 41.0 31.0 41.0 22 36.739605405951814 40.0 37.0 41.0 30.0 41.0 23 36.6462247597323 40.0 37.0 41.0 30.0 41.0 24 36.51912223851874 40.0 37.0 41.0 30.0 41.0 25 36.04983444940122 39.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03323352615109698 1101 2 0.18201905697960186 1101 3 0.1986877455158691 1101 4 0.179373375347204 1101 5 0.2873998191413776 1101 6 0.22110399146529858 1101 7 0.24423660193846075 1101 8 0.1966389820346066 1101 9 0.18039777036317162 1101 10 0.38726227678231595 1101 11 0.3729804676997617 1101 12 0.290930382193622 1101 13 0.33537424257580284 1101 14 0.2592776477869947 1101 15 0.10943854197321912 1101 16 -0.030882133429557257 1101 17 0.010494529743503733 1101 18 0.08579955847417153 1101 19 0.12659493303805647 1101 20 -0.07942208582885968 1101 21 0.02057375706801423 1101 22 0.2065165605881063 1101 23 0.0872681798826278 1101 24 -0.15425754901114885 1101 25 0.09929687807534293 1102 1 0.42272624544374793 1102 2 0.4241355241783715 1102 3 0.4600216967488855 1102 4 0.4694479884564444 1102 5 0.5121473330426838 1102 6 0.5518778648751095 1102 7 0.6398239023772518 1102 8 0.6505526242373847 1102 9 0.6373730595442453 1102 10 0.6621791101268499 1102 11 0.7195285849146416 1102 12 0.8017338642535705 1102 13 0.7404192556487459 1102 14 0.853960150142882 1102 15 0.8155158380074639 1102 16 0.8882436418881099 1102 17 1.0191304817425788 1102 18 1.0507736623904265 1102 19 1.0395643946671171 1102 20 1.0397197112370336 1102 21 0.8534143785740227 1102 22 0.9331588328510136 1102 23 1.0645164360100878 1102 24 0.8864959785011735 1102 25 1.0698826136659676 1103 1 0.3411167914539419 1103 2 0.35001099532409796 1103 3 0.36246032293624 1103 4 0.36969446051892874 1103 5 0.561104427201542 1103 6 0.44842628748988744 1103 7 0.3347107485393295 1103 8 0.47588841371310764 1103 9 0.41634044481796195 1103 10 0.5733379272933945 1103 11 0.3673015505792847 1103 12 0.5647255320201836 1103 13 0.5660743768412217 1103 14 0.7606062622515566 1103 15 0.6451191610496849 1103 16 0.8277942032505763 1103 17 0.8480754944259132 1103 18 0.8718662694184403 1103 19 1.0224945371183907 1103 20 0.8779187024919395 1103 21 0.9285646983069782 1103 22 0.9437142126911127 1103 23 0.8637168338188204 1103 24 0.9333367390640959 1103 25 0.8824232738081932 1104 1 0.21658816161112426 1104 2 0.2900872863233772 1104 3 0.29468607261491186 1104 4 0.3623215115088385 1104 5 0.4067874904866926 1104 6 0.43143610920938613 1104 7 0.3228707642958426 1104 8 0.3255598436567766 1104 9 0.4243989742264773 1104 10 0.3670565255989757 1104 11 0.4735538662293237 1104 12 0.45054975813902587 1104 13 0.49131167295302447 1104 14 0.56603999813467 1104 15 0.6792762940320998 1104 16 0.6921472244188109 1104 17 0.5462742852967182 1104 18 0.7073428486951272 1104 19 0.5208582116894149 1104 20 0.4438421817102167 1104 21 0.3504186228646162 1104 22 0.5894174021198282 1104 23 0.6066863386753241 1104 24 0.8004040989526402 1104 25 0.8169772523673942 1105 1 0.34284281725155097 1105 2 0.37235388471900777 1105 3 0.44182418351793373 1105 4 0.43243259222531094 1105 5 0.5004661069227083 1105 6 0.42877774729339535 1105 7 0.37074933537370924 1105 8 0.3131868874040151 1105 9 0.31761091604646197 1105 10 0.4870568996868485 1105 11 0.49163166247769396 1105 12 0.4209710979814858 1105 13 0.3796838529734643 1105 14 0.5268449417058534 1105 15 0.4574398499243344 1105 16 0.44296963321735916 1105 17 0.7028861714711283 1105 18 0.6447392438909034 1105 19 0.5039424258827765 1105 20 0.46420892810436243 1105 21 0.5683809194835661 1105 22 0.5295890616905155 1105 23 0.7173882911801925 1105 24 0.8339761471071014 1105 25 0.9165430766349374 1106 1 0.2976545769533381 1106 2 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0.5934205788158309 1107 15 0.58105938359558 1107 16 0.7366570358587694 1107 17 0.6231639744487438 1107 18 0.6690251645752667 1107 19 0.784983129948067 1107 20 0.6996702388627796 1107 21 0.7551856140447697 1107 22 0.7153239909616858 1107 23 0.6860415060458749 1107 24 0.7133973934883358 1107 25 0.6767282682816216 1108 1 0.2427427955076915 1108 2 0.23320044556556851 1108 3 0.19853143871834078 1108 4 0.20265945313710176 1108 5 0.3339610695957589 1108 6 0.30478992385351944 1108 7 0.29890763065529313 1108 8 0.37453065778888117 1108 9 0.42301136541951223 1108 10 0.49789113901423576 1108 11 0.4408503332032154 1108 12 0.4493055185076855 1108 13 0.4220336391588617 1108 14 0.6527527477506467 1108 15 0.5454872515333804 1108 16 0.55323273389142 1108 17 0.6135297896489931 1108 18 0.7279624083469471 1108 19 0.6571510944339067 1108 20 0.5386040864721195 1108 21 0.6857028010520168 1108 22 0.6001188870148582 1108 23 0.5802961697045106 1108 24 0.7852735305962639 1108 25 0.5951492953781425 1109 1 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-1.2232409150462118 2314 19 -1.368639711845276 2314 20 -1.3542035430783201 2314 21 -1.4192247107167688 2314 22 -1.2835004606848344 2314 23 -1.2499628374036504 2314 24 -1.16585739834089 2314 25 -1.2048790247328824 2315 1 -0.8424368990116236 2315 2 -0.8134864367345216 2315 3 -0.8246918317219105 2315 4 -0.7937174561171645 2315 5 -0.9473012844024495 2315 6 -0.9069553591653445 2315 7 -0.9639068347535158 2315 8 -1.0406782370898284 2315 9 -1.0633116354789607 2315 10 -1.247881547418828 2315 11 -1.2958792265452175 2315 12 -1.2379990637025529 2315 13 -1.1793926860882138 2315 14 -1.3987746556635514 2315 15 -1.405184356732569 2315 16 -1.3068975446350848 2315 17 -1.4868520612045515 2315 18 -1.3053776635308623 2315 19 -1.3813417177853182 2315 20 -1.3522045388648039 2315 21 -1.547515977025249 2315 22 -1.4319116610534195 2315 23 -1.4104697781840798 2315 24 -1.3093580956391335 2315 25 -1.5313511538581537 2316 1 -0.840862115206594 2316 2 -0.8925622032439549 2316 3 -0.9071456446149924 2316 4 -0.8062616778152787 2316 5 -1.0051088708804343 2316 6 -1.0870850761464794 2316 7 -1.1228371610113754 2316 8 -1.6893049076873083 2316 9 -1.4208465214852453 2316 10 -1.3438573728905254 2316 11 -1.2911548751301183 2316 12 -1.362792695778026 2316 13 -1.6748599620316114 2316 14 -1.5087613891541167 2316 15 -1.637031349657093 2316 16 -1.613545539918107 2316 17 -1.657935984160531 2316 18 -1.6605122940340067 2316 19 -1.6568109591375162 2316 20 -1.6996101992421657 2316 21 -1.5352272584717923 2316 22 -1.6064473332232367 2316 23 -1.6420661814859656 2316 24 -1.7071371836894471 2316 25 -1.8853826986694742 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22460.0 3 1.0 4 418.0 5 3670.0 6 4848.0 7 3922.0 8 3235.0 9 2900.0 10 2737.0 11 2679.0 12 2592.0 13 2408.0 14 2464.0 15 2363.0 16 2504.0 17 2605.0 18 2846.0 19 3196.0 20 3857.0 21 5089.0 22 6678.0 23 8770.0 24 10376.0 25 12379.0 26 14451.0 27 16818.0 28 19422.0 29 24155.0 30 31470.0 31 40094.0 32 52141.0 33 69542.0 34 91961.0 35 125887.0 36 188132.0 37 347492.0 38 960682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48045602293905 19.779334162071258 13.627487410909078 25.112722404080607 2 14.14861599317962 20.704982348262767 42.242342807989914 22.904058850567697 3 18.579478591904422 24.857288899145736 30.31449845606901 26.248734052880828 4 12.02034670262497 17.626418289908088 39.294998579087604 31.058236428379338 5 12.54013679420771 38.095326674870506 36.70387011227809 12.660666418643697 6 30.60530868129793 38.546301717873554 18.43476486283904 12.413624737989476 7 27.153254461569563 31.81999805458974 22.92356063481407 18.103186849026628 8 23.916816545905007 38.76749677195405 20.84969607732815 16.46599060481279 9 25.81793057936988 15.697172098239404 20.821659282372483 37.66323804001823 10 14.145278279494422 29.798774010081804 35.433263845313185 20.62268386511059 11 33.8472776653551 22.68028898878719 25.372536528892205 18.0998968169655 12 22.80974459814881 24.851567104256826 32.92625941473668 19.412428882857693 13 28.718070000438672 22.410935494391687 26.26575639267534 22.605238112494302 14 19.954378222085747 22.938723391269686 28.473177179193264 28.633721207451302 15 22.079023709210755 32.067370320287004 23.86327008206961 21.99033588843263 16 21.151139304725632 28.92305330233392 28.846285887574357 21.07952150536609 17 20.78565965619642 29.204756337364657 28.814529925940903 21.195054080498025 18 20.558265990986264 28.04366110953232 31.207432230107703 20.19064066937371 19 22.778131681387574 27.316277934279466 28.36570279852988 21.539887585803083 20 22.642286734400003 28.05081335314346 29.20008353820538 20.10681637425116 21 21.99448418972709 27.330487058253595 28.10769753066405 22.567331221355264 22 22.062478185656985 28.967730984091506 28.46707393131176 20.502716898939752 23 21.350782264724565 28.485479038204424 28.82525829135761 21.338480405713405 24 22.232796946850247 27.331726780479528 29.746801039840857 20.68867523282937 25 21.936872822984988 27.98051683319863 29.12809729185889 20.954513051957495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 189.0 2 372.5 3 556.0 4 556.0 5 556.0 6 1510.0 7 2464.0 8 2464.0 9 2464.0 10 3695.5 11 4927.0 12 4927.0 13 4927.0 14 8022.0 15 11117.0 16 11117.0 17 11117.0 18 20163.0 19 29209.0 20 29209.0 21 29209.0 22 46353.5 23 63498.0 24 63498.0 25 63498.0 26 90487.0 27 117476.0 28 117476.0 29 117476.0 30 150491.0 31 183506.0 32 183506.0 33 183506.0 34 204209.5 35 224913.0 36 224913.0 37 224913.0 38 237193.5 39 249474.0 40 249474.0 41 249474.0 42 258168.0 43 266862.0 44 266862.0 45 266862.0 46 267472.5 47 268083.0 48 268083.0 49 268083.0 50 254111.5 51 240140.0 52 240140.0 53 240140.0 54 208380.5 55 176621.0 56 176621.0 57 176621.0 58 148622.0 59 120623.0 60 120623.0 61 120623.0 62 96982.0 63 73341.0 64 73341.0 65 73341.0 66 55840.0 67 38339.0 68 38339.0 69 38339.0 70 27557.5 71 16776.0 72 16776.0 73 16776.0 74 11499.5 75 6223.0 76 6223.0 77 6223.0 78 4141.0 79 2059.0 80 2059.0 81 2059.0 82 1350.5 83 642.0 84 642.0 85 642.0 86 403.5 87 165.0 88 165.0 89 165.0 90 100.0 91 35.0 92 35.0 93 35.0 94 20.5 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42570153973500463 2 0.0 3 0.0 4 0.0 5 0.031660598385309484 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.002336399579638802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2097244.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.99283392219529 #Duplication Level Percentage of deduplicated Percentage of total 1 85.44005193516094 63.21951573142104 2 10.675003934141838 15.797475864354768 3 2.2224475948940876 4.933355873693417 4 0.6909933521830084 2.0451422539767337 5 0.3163957439664653 1.1705508868500045 6 0.15790867189074806 0.7010466080451929 7 0.0994368438819462 0.5150329712572873 8 0.06724426701215203 0.39804751049999365 9 0.04775956932115491 0.31804792928782094 >10 0.24367981589957757 3.303793428062522 >50 0.018907546282603424 0.9758029342772435 >100 0.016306299191920736 2.458189029037083 >500 0.00206102729258677 1.0951496027796006 >1k 0.0016101775723333486 2.2531106579726883 >5k 1.9322130868000182E-4 0.8157387184846758 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6552 0.31241000093455984 No Hit GTCCTACAGTGGACATTTCTAAATT 5417 0.2582913576102733 No Hit CTGTAGGACGTGGAATATGGCAAGA 5148 0.245465000734297 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4420 0.2107527784082348 No Hit GGTATCAACGCAGAGTACTTTTTTT 4059 0.1935397121174265 No Hit TATCAACGCAGAGTACTTTTTTTTT 3994 0.19044040655259953 No Hit CTTTAGGACGTGAAATATGGCGAGG 3839 0.183049754821089 No Hit CTGAAGGACCTGGAATATGGCGAGA 2280 0.1087141028893157 No Hit GTCCTACAGTGTGCATTTCTCATTT 2240 0.10680683792634524 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2109 0.100560545172617 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.768162407426127E-5 0.0 4 0.0 0.0 0.0 4.768162407426127E-5 0.0 5 0.0 0.0 0.0 4.768162407426127E-5 0.0 6 0.0 0.0 0.0 4.768162407426127E-5 0.0 7 4.768162407426127E-5 0.0 0.0 4.768162407426127E-5 0.0 8 4.768162407426127E-5 0.0 0.0 4.768162407426127E-5 0.0 9 4.768162407426127E-5 0.0 0.0 4.768162407426127E-5 0.0 10 4.768162407426127E-5 0.0 0.0 4.768162407426127E-5 0.0 11 4.768162407426127E-5 0.0 0.0 1.430448722227838E-4 0.0 12 9.536324814852254E-5 0.0 0.0 1.430448722227838E-4 0.0 13 9.536324814852254E-5 0.0 0.0 1.430448722227838E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGTAT 40 0.0051474157 14.304689 1 GGTATCA 1115 0.0 12.829318 1 TAGGACC 760 0.0 12.750096 4 GGCGAGG 1140 0.0 11.080774 19 TATGTCG 95 1.3649196E-5 10.996937 16 GCCCTAT 105 3.3119231E-6 10.898811 1 GACCGTG 115 8.8518937E-7 10.736139 7 CGGACAG 80 3.7715957E-4 10.68758 5 GGACGTG 2635 0.0 10.524582 6 GTCCTAC 2650 0.0 10.436127 1 GTATCAA 2500 0.0 10.413814 1 AGGACGT 2730 0.0 10.370042 5 CCACCTT 725 0.0 10.348841 13 TGTAGGA 2650 0.0 10.324607 2 GACGTGG 1355 0.0 10.303402 7 CTGTAGG 2645 0.0 10.27558 1 GTAGGAC 2685 0.0 10.154639 3 AATCCCG 375 0.0 10.130994 19 GGACCGT 75 0.0026543587 10.13051 6 GTCCTAG 265 0.0 10.07626 1 >>END_MODULE