FastQCFastQC Report
Thu 26 May 2016
SRR1511700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273269
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64250.28263263168591135No Hit
GGTATCAACGCAGAGTACTTTTTTT38160.16786398793983467No Hit
TATCAACGCAGAGTACTTTTTTTTT37640.16557653317755178No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC658.3412527E-411.6387086
GTCAACG853.5432662E-511.63182
GGTATCA14650.011.4730041
GCGTTAC700.00106863511.2994631
AATCCCG2500.010.21296619
GTTATAC1655.313268E-910.1866371
ACACCGA750.002751243810.086886
TGTACCG750.002751243810.086885
GTATCAA28150.09.9748371
GTTCTAG2900.09.8870311
GTACTAC2550.09.6931671
TAATACT3450.09.59354
AGCGTGT1107.190831E-59.456458
CTAGACA2107.2759576E-119.456454
ACTGTTC7100.09.456458
CGGTTTC1502.8319482E-79.456449513
CCGGTTT1502.8319482E-79.456449512
GCGTGCG1401.1273314E-69.45644959
GGCGTGC1752.8776412E-89.1862668
GTCCTAA2800.09.1808141
Produced by FastQC (version 0.11.5)