FastQCFastQC Report
Thu 26 May 2016
SRR1511700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273269
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83520.3674004264343551No Hit
TATCAACGCAGAGTACTTTTTTTTT50110.2204314579576812No Hit
GGTATCAACGCAGAGTACTTTTTTT47740.2100059429834305No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24740.1088300592670731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAACG400.005280096514.254
GGTATCA16200.012.482081
ATTAGAC855.325138E-511.1764713
GTATCAA32950.010.0447731
GTCCTAG3050.010.0072841
GGCGAAC1252.5803656E-69.87717510
CGGTTTC1752.586603E-99.76863513
GTGCTAC1502.499164E-79.5381931
CTAGTAC900.00111393049.53
TTATACT3100.09.4999994
GCACCGT1106.859849E-59.4972846
GTCCTAA2850.09.3708561
GTAGGAC3800.09.253
GATATAC5600.09.1975431
ACCGTGC1451.7133934E-69.1697918
GTCTTAA2950.09.0532011
GCGAACC1054.506346E-49.045032511
TAGGACC2850.09.04
TGTAGAA7400.08.9864862
GTGGTAT5850.08.8044861
Produced by FastQC (version 0.11.5)