##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511700_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2273269 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28815639504168 33.0 31.0 34.0 28.0 34.0 2 31.458456082408198 33.0 31.0 34.0 28.0 34.0 3 31.572131146819846 34.0 31.0 34.0 28.0 34.0 4 35.12762853846157 37.0 35.0 37.0 32.0 37.0 5 34.80739850849152 37.0 35.0 37.0 32.0 37.0 6 34.76769577203578 37.0 35.0 37.0 32.0 37.0 7 34.61333832467693 37.0 35.0 37.0 31.0 37.0 8 34.53768779673677 37.0 35.0 37.0 31.0 37.0 9 36.09228296343284 39.0 37.0 39.0 32.0 39.0 10 35.922920692623705 39.0 37.0 39.0 30.0 39.0 11 35.9238119201907 39.0 37.0 39.0 30.0 39.0 12 35.76863890723007 39.0 35.0 39.0 30.0 39.0 13 35.743798028301974 39.0 35.0 39.0 30.0 39.0 14 36.85130532286324 40.0 37.0 41.0 31.0 41.0 15 36.76546374406197 40.0 37.0 41.0 30.0 41.0 16 36.61637052192239 40.0 36.0 41.0 30.0 41.0 17 36.59716382003186 40.0 36.0 41.0 30.0 41.0 18 36.53122397745273 40.0 36.0 41.0 30.0 41.0 19 36.54411862388481 40.0 36.0 41.0 30.0 41.0 20 36.644382165067135 40.0 37.0 41.0 30.0 41.0 21 36.6026968211857 40.0 37.0 41.0 30.0 41.0 22 36.50588645690413 40.0 37.0 41.0 30.0 41.0 23 36.410602968676386 40.0 37.0 41.0 30.0 41.0 24 36.26016806633971 40.0 36.0 41.0 30.0 41.0 25 35.7440984766871 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17218506237471942 1101 2 0.23989168564747132 1101 3 0.30050485450616193 1101 4 0.1923366188344744 1101 5 0.3565618581386403 1101 6 0.3563041093647854 1101 7 0.32516225140623334 1101 8 0.2542054777361926 1101 9 0.30606309041745305 1101 10 0.41009116392718425 1101 11 0.3402586076744072 1101 12 0.4700880729458632 1101 13 0.22606108894165544 1101 14 0.24003830322050135 1101 15 0.1784863942493331 1101 16 0.058667341950339846 1101 17 0.11846507088662861 1101 18 0.2557149545868356 1101 19 0.015973384193316065 1101 20 -0.09060292492006994 1101 21 0.07713762983061656 1101 22 0.15973851397183125 1101 23 0.01908429238392273 1101 24 0.010984549795168164 1101 25 0.06638474703276387 1102 1 0.4359123605684836 1102 2 0.40378205859511596 1102 3 0.3072809931962368 1102 4 0.44348989193219523 1102 5 0.48237079281012996 1102 6 0.5388308261018793 1102 7 0.48675809780787205 1102 8 0.5460563026030343 1102 9 0.5027207448000368 1102 10 0.5449994638140083 1102 11 0.5293740546900807 1102 12 0.7464855534310999 1102 13 0.7468923678961303 1102 14 0.6880957561635768 1102 15 0.7012653470091692 1102 16 0.7555258961876632 1102 17 0.6589563262237235 1102 18 0.5683649242433546 1102 19 0.6866484812949167 1102 20 0.67947737124409 1102 21 0.46262250494154955 1102 22 0.6061789561183133 1102 23 0.7676499066689573 1102 24 0.7829048335177689 1102 25 0.8439152634508744 1103 1 0.31070634063995684 1103 2 0.4462857929297357 1103 3 0.4345262410349946 1103 4 0.3885420296302442 1103 5 0.5096197583017315 1103 6 0.3959156715190133 1103 7 0.4149132650116911 1103 8 0.4048627072609676 1103 9 0.45093765363842664 1103 10 0.6048722628920302 1103 11 0.4586130606850318 1103 12 0.5836878671457129 1103 13 0.6084322006398821 1103 14 0.8164277025264255 1103 15 0.6921971337989206 1103 16 0.7222888881928 1103 17 0.8624956747193622 1103 18 0.7979283192801958 1103 19 0.8538148862960142 1103 20 0.8100637404222724 1103 21 0.9366725731461827 1103 22 0.9905110988494954 1103 23 0.909165460279894 1103 24 0.9478132410373377 1103 25 1.0326571620657106 1104 1 0.2916031530984462 1104 2 0.4247365632251743 1104 3 0.4307637066074079 1104 4 0.5159851689615209 1104 5 0.45385334104636854 1104 6 0.5244131193493189 1104 7 0.45127645803374605 1104 8 0.4237752368117924 1104 9 0.6139309608694958 1104 10 0.6830485414152534 1104 11 0.6197144182813972 1104 12 0.599244490943363 1104 13 0.6024737320345892 1104 14 0.8001462996923294 1104 15 0.8061163282514912 1104 16 0.8318986547122336 1104 17 0.7335864892307313 1104 18 0.733620377596921 1104 19 0.6450987275087954 1104 20 0.6755400138365104 1104 21 0.60643412293323 1104 22 0.7486278809327516 1104 23 0.7164766484902145 1104 24 0.8628350471095985 1104 25 0.8145244492417021 1105 1 0.19493886710733932 1105 2 0.24747699761541853 1105 3 0.35023597173715615 1105 4 0.2707622989740912 1105 5 0.13197382537543945 1105 6 0.2743281347683464 1105 7 0.37386685449927626 1105 8 0.27090486182770945 1105 9 0.2385305367770627 1105 10 0.32666362493267087 1105 11 0.4411892191074358 1105 12 0.4288977427800802 1105 13 0.5442125110248526 1105 14 0.5273275244668625 1105 15 0.4211389446692948 1105 16 0.5861885767035915 1105 17 0.5831333304064543 1105 18 0.5205908849022762 1105 19 0.4610005329466986 1105 20 0.5683642147876782 1105 21 0.5617460744781795 1105 22 0.4589561373370472 1105 23 0.6027168974092234 1105 24 0.6039116661073933 1105 25 0.7262645016165621 1106 1 0.10568581842215252 1106 2 0.3324968345437398 1106 3 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1107 15 0.7445978754630218 1107 16 0.8150001234465165 1107 17 0.9009685794906446 1107 18 0.9030313107937786 1107 19 0.8354468235160297 1107 20 0.742349144848319 1107 21 0.7310177526056947 1107 22 0.7420080524660264 1107 23 0.7953504933215072 1107 24 1.0795120164077545 1107 25 1.045164918421321 1108 1 0.24750080666508367 1108 2 0.34149186917588636 1108 3 0.26339183234320984 1108 4 0.32214187730290433 1108 5 0.3642958828345968 1108 6 0.26448683935899453 1108 7 0.46062396942909345 1108 8 0.5245590002489067 1108 9 0.46566145943511117 1108 10 0.5378644684005565 1108 11 0.6410015726066618 1108 12 0.4313660159825048 1108 13 0.47294993457773415 1108 14 0.533642446338348 1108 15 0.5702317250904585 1108 16 0.6650097299241722 1108 17 0.6192727494553978 1108 18 0.5700333321306204 1108 19 0.5489362436238281 1108 20 0.6382528088300532 1108 21 0.7254445305261186 1108 22 0.8098226687950287 1108 23 0.6293673027737796 1108 24 0.7987132095364657 1108 25 0.7845570858142992 1109 1 0.027459721519136338 1109 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-1.0536349326845382 2314 22 -0.9802634964468666 2314 23 -0.8622452004583963 2314 24 -0.9218996175662753 2314 25 -1.1394618018470126 2315 1 -0.8522824271171316 2315 2 -0.9396911637120127 2315 3 -0.8933298060993415 2315 4 -0.859723647399889 2315 5 -1.079925452693736 2315 6 -0.9964313526178472 2315 7 -1.0254757584375866 2315 8 -1.1477783275935067 2315 9 -1.3993744080157242 2315 10 -1.4113287526761127 2315 11 -1.4089788723747247 2315 12 -1.3081418780021679 2315 13 -1.2854707196303181 2315 14 -1.5323129322707274 2315 15 -1.6799606555296407 2315 16 -1.552844411302381 2315 17 -1.6715390237410546 2315 18 -1.6978277339958368 2315 19 -1.545939057625361 2315 20 -1.5850417520181495 2315 21 -1.5005224157952384 2315 22 -1.518135455059415 2315 23 -1.471986912355817 2315 24 -1.5661866474238266 2315 25 -1.579053921294033 2316 1 -0.9270182556372433 2316 2 -1.0730495765334638 2316 3 -1.1086578714325839 2316 4 -1.1084266534341367 2316 5 -1.1701037341185483 2316 6 -1.2817659715691576 2316 7 -1.1967693160688881 2316 8 -1.8676991559122484 2316 9 -1.3889580519005875 2316 10 -1.5448840878599412 2316 11 -1.5347938249799853 2316 12 -1.7075416178449743 2316 13 -1.7816132119194918 2316 14 -1.7078285524244876 2316 15 -1.8813401842863229 2316 16 -1.760607048984582 2316 17 -1.6660072836743822 2316 18 -1.806331824907538 2316 19 -1.7485771373368166 2316 20 -1.8257385433604725 2316 21 -1.7724575532826137 2316 22 -1.7725207042407334 2316 23 -1.7906955566983456 2316 24 -1.8192300524599148 2316 25 -1.981897448824725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26558.0 3 1.0 4 466.0 5 4357.0 6 5601.0 7 4606.0 8 3856.0 9 3391.0 10 3083.0 11 3093.0 12 3024.0 13 2823.0 14 2855.0 15 2859.0 16 2871.0 17 3078.0 18 3178.0 19 3798.0 20 4549.0 21 5699.0 22 7651.0 23 9347.0 24 11728.0 25 14174.0 26 16676.0 27 19023.0 28 22273.0 29 27533.0 30 35212.0 31 45738.0 32 59182.0 33 78029.0 34 103325.0 35 140840.0 36 209401.0 37 385742.0 38 997649.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.807678630198104 18.932934573494105 13.690509059343503 24.568877736964286 2 15.43913192851352 20.570068918372616 39.68272122656843 24.30807792654543 3 18.869742208247242 24.69672528856022 29.767440632850757 26.666091870341784 4 12.969736533599852 17.218815723084248 37.56062304988983 32.250824693426075 5 13.870551551703958 37.30518443288417 35.1049093214887 13.719354693923172 6 31.92481840028611 36.889211087645144 17.961534688591627 13.224435823477116 7 28.137541135694892 31.674474072360116 21.867891569365526 18.320093222579466 8 25.779043307237288 35.62899947168593 20.324343489485848 18.267613731590938 9 26.288485876506474 15.763730557184388 19.873582932772145 38.07420063353699 10 15.2173367956014 29.101967255085075 33.44566789060159 22.235028058711926 11 34.75356414045148 22.995342830083022 23.32381253604391 18.927280493421588 12 23.031458221618294 24.784440380790834 30.996639640975175 21.187461756615694 13 28.757573344817526 21.540433622241803 25.823956601704417 23.87803643123625 14 21.786686925304487 21.135026255141824 27.670636427101236 29.407650392452457 15 23.781215509471163 28.74969922169352 23.976352996499752 23.492732272335566 16 23.363315120207947 28.00843191016989 25.85527713614183 22.772975833480334 17 22.462277891441794 27.509414855874954 27.011409560417178 23.016897692266074 18 22.25328370729553 27.47127594666535 27.955336565976136 22.320103780062983 19 23.353901364070857 26.914456670108112 27.615253628145197 22.116388337675833 20 23.57367297930865 26.581368065108002 27.7384242691912 22.106534686392152 21 23.940633510596417 26.300275066435162 27.26210580446045 22.496985618507974 22 23.43765739998214 26.382755406421328 27.48992750088089 22.689659692715644 23 23.547191291483763 26.36775498192251 27.060369890232966 23.024683836360765 24 23.00224918388453 26.484855069945528 27.53558861709723 22.977307129072713 25 23.46656249752554 26.3197323979226 27.47575472038416 22.7379503841677 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 277.0 1 277.0 2 481.5 3 686.0 4 686.0 5 686.0 6 1714.5 7 2743.0 8 2743.0 9 2743.0 10 3833.0 11 4923.0 12 4923.0 13 4923.0 14 7624.0 15 10325.0 16 10325.0 17 10325.0 18 18179.0 19 26033.0 20 26033.0 21 26033.0 22 40343.5 23 54654.0 24 54654.0 25 54654.0 26 76714.5 27 98775.0 28 98775.0 29 98775.0 30 124043.0 31 149311.0 32 149311.0 33 149311.0 34 169902.0 35 190493.0 36 190493.0 37 190493.0 38 211760.0 39 233027.0 40 233027.0 41 233027.0 42 251686.5 43 270346.0 44 270346.0 45 270346.0 46 282248.5 47 294151.0 48 294151.0 49 294151.0 50 292594.0 51 291037.0 52 291037.0 53 291037.0 54 266194.0 55 241351.0 56 241351.0 57 241351.0 58 209495.5 59 177640.0 60 177640.0 61 177640.0 62 147000.5 63 116361.0 64 116361.0 65 116361.0 66 90258.0 67 64155.0 68 64155.0 69 64155.0 70 47021.5 71 29888.0 72 29888.0 73 29888.0 74 20715.0 75 11542.0 76 11542.0 77 11542.0 78 7771.0 79 4000.0 80 4000.0 81 4000.0 82 2597.5 83 1195.0 84 1195.0 85 1195.0 86 741.5 87 288.0 88 288.0 89 288.0 90 171.5 91 55.0 92 55.0 93 55.0 94 33.5 95 12.0 96 12.0 97 12.0 98 6.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42159550849459526 2 0.0 3 0.0 4 0.0 5 0.03233229327457507 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.00189154913034929 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2273269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.69810655763315 #Duplication Level Percentage of deduplicated Percentage of total 1 78.62915863779737 54.01674318636446 2 13.980298185144441 19.208400288610765 3 4.010143701737174 8.264678380000857 4 1.4849504533720697 4.080531379142404 5 0.6927948297987716 2.379684652004667 6 0.36202048420121163 1.492207307982047 7 0.22920342039468256 1.1022088698353523 8 0.13411358541319637 0.73706795052336 9 0.08436987361337364 0.5216445510971026 >10 0.36468875749886054 4.242306062293662 >50 0.016846923530521824 0.7997220772887519 >100 0.009490333711272173 1.2452661085015693 >500 0.0012805425247001845 0.6331701699396082 >1k 5.122170098797909E-4 0.6885918034317732 >5k 1.2805425246994773E-4 0.5877772129836337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8352 0.3674004264343551 No Hit TATCAACGCAGAGTACTTTTTTTTT 5011 0.2204314579576812 No Hit GGTATCAACGCAGAGTACTTTTTTT 4774 0.2100059429834305 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2474 0.1088300592670731 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.797902931857162E-5 0.0 0.0 0.0 3 0.0 8.797902931857162E-5 0.0 0.0 0.0 4 0.0 8.797902931857162E-5 0.0 0.0 0.0 5 0.0 8.797902931857162E-5 0.0 4.398951465928581E-5 0.0 6 0.0 8.797902931857162E-5 0.0 4.398951465928581E-5 0.0 7 0.0 8.797902931857162E-5 0.0 4.398951465928581E-5 0.0 8 0.0 8.797902931857162E-5 0.0 1.3196854397785744E-4 0.0 9 0.0 8.797902931857162E-5 0.0 1.7595805863714325E-4 0.0 10 0.0 8.797902931857162E-5 0.0 1.7595805863714325E-4 0.0 11 0.0 8.797902931857162E-5 0.0 1.7595805863714325E-4 0.0 12 0.0 8.797902931857162E-5 0.0 1.7595805863714325E-4 0.0 13 0.0 8.797902931857162E-5 0.0 2.1994757329642906E-4 4.398951465928581E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAAACG 40 0.0052800965 14.25 4 GGTATCA 1620 0.0 12.48208 1 ATTAGAC 85 5.325138E-5 11.176471 3 GTATCAA 3295 0.0 10.044773 1 GTCCTAG 305 0.0 10.007284 1 GGCGAAC 125 2.5803656E-6 9.877175 10 CGGTTTC 175 2.586603E-9 9.768635 13 GTGCTAC 150 2.499164E-7 9.538193 1 CTAGTAC 90 0.0011139304 9.5 3 TTATACT 310 0.0 9.499999 4 GCACCGT 110 6.859849E-5 9.497284 6 GTCCTAA 285 0.0 9.370856 1 GTAGGAC 380 0.0 9.25 3 GATATAC 560 0.0 9.197543 1 ACCGTGC 145 1.7133934E-6 9.169791 8 GTCTTAA 295 0.0 9.053201 1 GCGAACC 105 4.506346E-4 9.0450325 11 TAGGACC 285 0.0 9.0 4 TGTAGAA 740 0.0 8.986486 2 GTGGTAT 585 0.0 8.804486 1 >>END_MODULE