FastQCFastQC Report
Thu 26 May 2016
SRR1511699_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511699_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194971
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57160.2604134633213833No Hit
GGTATCAACGCAGAGTACTTTTTTT36530.1664258889980779No Hit
TATCAACGCAGAGTACTTTTTTTTT35480.16164222670823442No Hit
GTCCTACAGTGGACATTTCTAAATT28970.13198352051120493No Hit
GTCCTAAAGTGTGTATTTCTCATTT25290.11521792315251547No Hit
CTGTAGGACGTGGAATATGGCAAGA23780.10833856119283582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1008.6298314E-812.8467911
CGTGCGC803.0041409E-511.8211410
TCCAACG2050.011.53308318
CGTAGTA600.00438005911.5291722
GGTATCA11350.011.4058271
ACTGCGT600.006079174611.0330658
GTCTTAG2450.010.8905291
GTATCAA24650.010.8643481
CCAACGA1408.967618E-910.80814619
GTACTCG650.0079467910.6423121
GCGTGCG803.933788E-410.6390279
TAGGACC5900.010.41863254
ACTGATC1901.09139364E-109.9546458
GGCGTGC1054.3165506E-59.9072418
GTGTAGA5450.09.7914851
GTATAAG2052.0008883E-109.641121
GTCCTAC15800.09.5694211
CCAACGT900.00116093189.45712819
TGCACCG900.00116117379.4569135
GTGTTCG800.00468923949.4569129
Produced by FastQC (version 0.11.5)