##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511699_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2194971 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.125515097921568 31.0 29.0 34.0 22.0 34.0 2 26.492790109755436 31.0 25.0 34.0 10.0 34.0 3 28.13819271416342 31.0 27.0 34.0 16.0 34.0 4 32.600873997879695 35.0 32.0 37.0 25.0 37.0 5 33.04610584832328 35.0 35.0 37.0 27.0 37.0 6 33.2922380295685 37.0 35.0 37.0 27.0 37.0 7 33.320185095839534 37.0 35.0 37.0 27.0 37.0 8 33.337306506555215 37.0 35.0 37.0 27.0 37.0 9 34.90686619549871 39.0 35.0 39.0 28.0 39.0 10 34.77274278338985 39.0 35.0 39.0 27.0 39.0 11 34.78164130642273 39.0 35.0 39.0 27.0 39.0 12 34.683080095363444 39.0 35.0 39.0 27.0 39.0 13 34.67613512889236 39.0 35.0 39.0 27.0 39.0 14 35.80667535015269 40.0 36.0 41.0 27.0 41.0 15 35.73932685215431 40.0 36.0 41.0 27.0 41.0 16 35.67919530599721 40.0 36.0 41.0 27.0 41.0 17 35.64906370061381 40.0 36.0 41.0 27.0 41.0 18 35.58048101774465 40.0 36.0 41.0 27.0 41.0 19 35.609466822112914 40.0 36.0 41.0 27.0 41.0 20 35.53180839291271 40.0 36.0 41.0 26.0 41.0 21 35.437864554930336 40.0 36.0 41.0 25.0 41.0 22 35.33100437317851 40.0 36.0 41.0 25.0 41.0 23 35.21881974750464 40.0 36.0 41.0 25.0 41.0 24 35.108460658477945 40.0 36.0 41.0 24.0 41.0 25 34.998359431628025 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3154202543756348 1101 2 3.748698658807193 1101 3 2.302028792002094 1101 4 1.2909281672831128 1101 5 0.9870926112135763 1101 6 0.7738069199539481 1101 7 0.6248111100521143 1101 8 0.39057576887598344 1101 9 0.4873368300236649 1101 10 0.7725144184078658 1101 11 0.6408715222803139 1101 12 0.7312173010121299 1101 13 0.6790016854989176 1101 14 0.8979393329647536 1101 15 0.761467630783315 1101 16 0.8733309384374124 1101 17 0.9203813102556921 1101 18 0.8327089007952679 1101 19 0.9265683993143199 1101 20 0.9378264327652701 1101 21 0.9112979144746873 1101 22 0.8235093914576623 1101 23 0.8616106068149563 1101 24 0.9554985044583262 1101 25 0.8761940242529676 1102 1 1.495549573039984 1102 2 4.041681067698356 1102 3 2.6821744943222896 1102 4 1.7233332430743502 1102 5 1.2652141465192273 1102 6 0.972510366805821 1102 7 0.8757986180756205 1102 8 0.8032295620289602 1102 9 0.9679513742213857 1102 10 0.9744631757187889 1102 11 0.9109560888765103 1102 12 0.9659412251894679 1102 13 1.0423810726155338 1102 14 1.0716655598312599 1102 15 1.0803047951989129 1102 16 1.2379290002284833 1102 17 1.036999274822108 1102 18 1.0023122816636487 1102 19 1.0428418582407204 1102 20 1.0427278390664725 1102 21 0.9707481418259647 1102 22 0.9065330063654429 1102 23 0.887308258790199 1102 24 0.607602794282954 1102 25 0.5919959480981447 1103 1 1.6451923294562683 1103 2 4.200103511515572 1103 3 2.7364770494665756 1103 4 1.6139323147657478 1103 5 1.1670497751170785 1103 6 0.9063913351676831 1103 7 0.8606835414781315 1103 8 0.761248521737393 1103 9 1.0168182080681376 1103 10 0.9686333714594184 1103 11 0.9878208500983092 1103 12 1.1818948622308554 1103 13 1.1542918885257691 1103 14 1.3263584812048776 1103 15 1.117336022310944 1103 16 1.1523495944246989 1103 17 1.4066829808854067 1103 18 1.3809560459703363 1103 19 1.4760428831843697 1103 20 1.4231982680867148 1103 21 1.5012663417905472 1103 22 1.4600162406223518 1103 23 1.4057547301899405 1103 24 1.5466997594615464 1103 25 1.2778784862329147 1104 1 1.4399940174844303 1104 2 3.982977755506674 1104 3 2.6398207240615434 1104 4 1.6941225313055028 1104 5 1.1868256099911605 1104 6 1.0987377569867007 1104 7 1.0235082727620153 1104 8 0.9097130010705428 1104 9 0.9680805731877911 1104 10 0.9321328961792119 1104 11 1.0944068758156646 1104 12 0.9323142578415684 1104 13 1.0543378496885865 1104 14 1.2304041081046933 1104 15 1.254840853455903 1104 16 0.9788028550558252 1104 17 1.0833582130415067 1104 18 0.9623428196011616 1104 19 1.1310495162158247 1104 20 0.9119080128978041 1104 21 1.2273372891127892 1104 22 1.1537963373497035 1104 23 1.1259739858280184 1104 24 1.2238701186898169 1104 25 1.1133490136372615 1105 1 1.7910041184945307 1105 2 4.126889519773293 1105 3 2.7469013877710893 1105 4 1.8384890650510428 1105 5 1.478767204347733 1105 6 1.2700676154530797 1105 7 1.1401458162600804 1105 8 0.9980450649324197 1105 9 1.1464542558000161 1105 10 0.9425256357981695 1105 11 1.081729087583419 1105 12 0.9684130476121169 1105 13 0.9706786853492844 1105 14 0.9652787291301266 1105 15 1.0166295138399306 1105 16 0.8728893583340493 1105 17 1.1608305709229327 1105 18 1.047346130183577 1105 19 1.0626859893752112 1105 20 0.9718118815498258 1105 21 1.1213506110955507 1105 22 1.0419481860486215 1105 23 1.1075380244398687 1105 24 1.1570319489191618 1105 25 1.1597758248743233 1106 1 1.2445439415033803 1106 2 3.751980839443384 1106 3 2.3231909305512204 1106 4 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17 0.881636610173345 1107 18 1.0053491541329223 1107 19 1.0405205979202066 1107 20 1.2309322533078912 1107 21 1.240469105783987 1107 22 1.2132054836481814 1107 23 1.271857043725035 1107 24 1.2338709243536314 1107 25 1.191514517126791 1108 1 1.1642142801573492 1108 2 3.739384781890603 1108 3 2.264916951929134 1108 4 1.1869456819808448 1108 5 0.8693599604238926 1108 6 0.5978302990353583 1108 7 0.5238301987190468 1108 8 0.505195540086568 1108 9 0.5533116367098501 1108 10 0.23645556863121442 1108 11 0.48867752689925226 1108 12 0.42772098096191513 1108 13 0.6219796254135019 1108 14 0.5687762825045795 1108 15 0.4602214492522023 1108 16 0.6433245150740206 1108 17 0.6814768124303185 1108 18 0.5794165593076954 1108 19 0.4776086409504643 1108 20 0.5923323486339243 1108 21 0.5696600548766639 1108 22 0.7849799984409884 1108 23 0.7228235437630275 1108 24 0.7394217084908234 1108 25 0.9091919697573516 1109 1 1.2525425800329906 1109 2 3.2212186073569917 1109 3 1.9950627207236593 1109 4 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-2.5356815081984294 2315 23 -2.4046114209554688 2315 24 -2.492896589929927 2315 25 -2.3905203509349846 2316 1 -0.8987240848235061 2316 2 1.4648237480833401 2316 3 0.1265368083067493 2316 4 -0.7761044472695176 2316 5 -1.072983208871669 2316 6 -1.255109932759332 2316 7 -1.6041388301057005 2316 8 -1.6332423198763095 2316 9 -1.7008797679575096 2316 10 -1.9192606724046186 2316 11 -1.8700709415389625 2316 12 -2.0375488633099437 2316 13 -1.963268143173316 2316 14 -2.2148461352394477 2316 15 -2.1642877784330565 2316 16 -2.2740509566225526 2316 17 -2.1737552798114805 2316 18 -2.414942565132158 2316 19 -2.371131667310223 2316 20 -2.121107814276158 2316 21 -2.5890833593057394 2316 22 -2.3560456404943935 2316 23 -2.5089179978991183 2316 24 -2.626769885303524 2316 25 -2.6355390908456187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 88665.0 3 1.0 4 746.0 5 3765.0 6 4507.0 7 4005.0 8 3824.0 9 3572.0 10 3525.0 11 3347.0 12 3242.0 13 3254.0 14 3243.0 15 3408.0 16 3709.0 17 3961.0 18 4441.0 19 5369.0 20 7133.0 21 9087.0 22 11082.0 23 13448.0 24 15357.0 25 17474.0 26 19982.0 27 22398.0 28 25960.0 29 30899.0 30 37883.0 31 48406.0 32 62016.0 33 81150.0 34 111654.0 35 157408.0 36 253309.0 37 319998.0 38 803743.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70599884918753 18.44753301979844 12.65711483204106 25.18935329897297 2 16.799576491266322 20.995864410555917 39.20883465116467 22.99572444701309 3 18.909315886177996 24.387019236245035 29.585447825962163 27.118217051614806 4 12.481303853217195 16.67634788796754 38.03043411507487 32.811914143740395 5 13.467558341317492 37.53051862644199 35.49778106407784 13.504141968162678 6 31.991447722999528 36.816522860666495 18.039554964507502 13.152474451826471 7 28.074903950895024 31.60638568801137 22.098469638095448 18.220240722998163 8 25.353637929612738 36.63073452906667 20.466830769062554 17.548796772258036 9 26.54818674141936 15.400385699856628 19.948509570285893 38.102917988438115 10 15.343163987132405 28.72525422887136 34.09493792856489 21.836643855431348 11 34.70296418494823 22.369270482389062 24.260183847531472 18.667581485131237 12 23.47192741954222 24.39494644803963 31.471304176683883 20.661821955734265 13 29.323804278051963 21.551628700333627 25.684029538431258 23.44053748318315 14 21.526616980361016 21.555273395411604 27.36364170642801 29.55446791779937 15 23.361493158679544 29.986455401916473 23.50240618213179 23.14964525727219 16 22.76658780457692 27.633166907444334 27.05211139463802 22.548133893340733 17 22.127854992161627 27.97007340871474 27.50391690824161 22.398154690882023 18 22.059516959449578 27.127511024063644 29.130134293346018 21.68283772314076 19 23.639730839145866 26.61922486412115 27.384884531451455 22.35615976528153 20 23.813389789660093 26.79853173458784 27.749432680431767 21.6386457953203 21 23.493916073338788 26.364805983150518 27.123725036037037 23.017552907473654 22 23.484844343121228 27.253484807895923 27.17175196222612 22.089918886756728 23 23.047173090704657 26.9247398242974 27.39536364916156 22.63272343583639 24 23.374579151423482 26.454074543160043 27.874822890517866 22.29652341489861 25 23.257619348957228 26.69912267633604 27.659955416267458 22.38330255843927 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 321.0 1 321.0 2 498.5 3 676.0 4 676.0 5 676.0 6 1577.0 7 2478.0 8 2478.0 9 2478.0 10 3499.5 11 4521.0 12 4521.0 13 4521.0 14 7176.5 15 9832.0 16 9832.0 17 9832.0 18 17262.5 19 24693.0 20 24693.0 21 24693.0 22 38610.0 23 52527.0 24 52527.0 25 52527.0 26 75158.0 27 97789.0 28 97789.0 29 97789.0 30 126317.0 31 154845.0 32 154845.0 33 154845.0 34 174872.0 35 194899.0 36 194899.0 37 194899.0 38 212068.0 39 229237.0 40 229237.0 41 229237.0 42 245219.5 43 261202.0 44 261202.0 45 261202.0 46 271787.0 47 282372.0 48 282372.0 49 282372.0 50 278395.5 51 274419.0 52 274419.0 53 274419.0 54 249915.0 55 225411.0 56 225411.0 57 225411.0 58 196032.0 59 166653.0 60 166653.0 61 166653.0 62 137328.0 63 108003.0 64 108003.0 65 108003.0 66 83977.5 67 59952.0 68 59952.0 69 59952.0 70 44131.0 71 28310.0 72 28310.0 73 28310.0 74 19660.5 75 11011.0 76 11011.0 77 11011.0 78 7489.5 79 3968.0 80 3968.0 81 3968.0 82 2642.5 83 1317.0 84 1317.0 85 1317.0 86 852.5 87 388.0 88 388.0 89 388.0 90 249.0 91 110.0 92 110.0 93 110.0 94 70.0 95 30.0 96 30.0 97 30.0 98 18.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.3018336005350415 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.555868847469967E-5 20 0.0 21 3.1891081932289766E-4 22 6.83380327120495E-4 23 5.46704261696396E-4 24 4.555868847469967E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2194971.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.02256047895663 #Duplication Level Percentage of deduplicated Percentage of total 1 79.95207408529484 55.18496868970284 2 13.208970284839952 18.234339007002134 3 3.732284240955518 7.7283544423802715 4 1.3829429928105976 3.8181706544087506 5 0.6231068273211817 2.1504214336813527 6 0.3227574342278048 1.3366526714412914 7 0.19625539076000456 0.9482234704637579 8 0.11219437151372075 0.6195154234563457 9 0.0817817473753832 0.5080307043862926 >10 0.34977358298933414 4.1118679379016925 >50 0.01980627107505683 0.9451448757713767 >100 0.015047184737377485 1.9518406320247177 >500 0.0019369332639127845 0.8904354424468217 >1k 0.001001862033057525 1.3085228888633054 >5k 6.6790802203835E-5 0.2635117260690056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5716 0.2604134633213833 No Hit GGTATCAACGCAGAGTACTTTTTTT 3653 0.1664258889980779 No Hit TATCAACGCAGAGTACTTTTTTTTT 3548 0.16164222670823442 No Hit GTCCTACAGTGGACATTTCTAAATT 2897 0.13198352051120493 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2529 0.11521792315251547 No Hit CTGTAGGACGTGGAATATGGCAAGA 2378 0.10833856119283582 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.555868847469967E-5 0.0 0.0 0.0 3 0.0 4.555868847469967E-5 0.0 0.0 0.0 4 0.0 9.111737694939934E-5 0.0 0.0 0.0 5 0.0 9.111737694939934E-5 0.0 0.0 4.555868847469967E-5 6 0.0 9.111737694939934E-5 0.0 0.0 9.111737694939934E-5 7 0.0 9.111737694939934E-5 0.0 0.0 9.111737694939934E-5 8 0.0 9.111737694939934E-5 0.0 0.0 9.111737694939934E-5 9 0.0 9.111737694939934E-5 0.0 0.0 9.111737694939934E-5 10 0.0 9.111737694939934E-5 0.0 0.0 9.111737694939934E-5 11 0.0 9.111737694939934E-5 0.0 0.0 9.111737694939934E-5 12 0.0 9.111737694939934E-5 0.0 0.0 1.8223475389879867E-4 13 0.0 2.73352130848198E-4 0.0 0.0 1.8223475389879867E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTAGG 100 8.6298314E-8 12.846791 1 CGTGCGC 80 3.0041409E-5 11.82114 10 TCCAACG 205 0.0 11.533083 18 CGTAGTA 60 0.004380059 11.529172 2 GGTATCA 1135 0.0 11.405827 1 ACTGCGT 60 0.0060791746 11.033065 8 GTCTTAG 245 0.0 10.890529 1 GTATCAA 2465 0.0 10.864348 1 CCAACGA 140 8.967618E-9 10.808146 19 GTACTCG 65 0.00794679 10.642312 1 GCGTGCG 80 3.933788E-4 10.639027 9 TAGGACC 590 0.0 10.4186325 4 ACTGATC 190 1.09139364E-10 9.954645 8 GGCGTGC 105 4.3165506E-5 9.907241 8 GTGTAGA 545 0.0 9.791485 1 GTATAAG 205 2.0008883E-10 9.64112 1 GTCCTAC 1580 0.0 9.569421 1 CCAACGT 90 0.0011609318 9.457128 19 TGCACCG 90 0.0011611737 9.456913 5 GTGTTCG 80 0.0046892394 9.456912 9 >>END_MODULE