FastQCFastQC Report
Thu 26 May 2016
SRR1511699_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511699_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194971
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66380.3024185740950564No Hit
TATCAACGCAGAGTACTTTTTTTTT42490.19357886732899887No Hit
GGTATCAACGCAGAGTACTTTTTTT40630.18510495127270474No Hit
GTCCTACAGTGGACATTTCTAAATT33130.15093593491668No Hit
CTGTAGGACGTGGAATATGGCAAGA27940.12729097559831087No Hit
GTCCTAAAGTGTGTATTTCTCATTT26320.11991046806540953No Hit
CTTTAGGACGTGAAATATGGCGAGG24780.11289443004030576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAC852.7105125E-713.40777716
GGTATCA12200.012.6672721
TAGGACC5800.011.3017114
GTATCAA27350.010.7428931
AGGACGT17900.010.6145135
GGACGTG17700.010.3556786
GACGTGA8350.010.3502157
GTATTAT2950.010.3479861
GGCGAGG6800.010.33539719
CCCCGTA1201.5301284E-610.28860715
GTCCTAA7550.010.2344851
TAGGACG18900.010.20369154
GTAGGAC17150.010.1370133
TCCAACG2250.010.13032118
GCCCTAA856.3480536E-410.10071
TGTAGGA17850.010.0588122
TTAGGAC10300.09.9611523
TAATACC1451.6177182E-79.8275754
TAAGACA4000.09.7374894
ATAAGAC2251.8189894E-129.71113
Produced by FastQC (version 0.11.5)