FastQCFastQC Report
Thu 26 May 2016
SRR1511698_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511698_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597203
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47430.29695661728659417No Hit
TATCAACGCAGAGTACTTTTTTTTT29180.18269437260010155No Hit
GGTATCAACGCAGAGTACTTTTTTT27160.17004726387315824No Hit
GTCCTAAAGTGTGTATTTCTCATTT16260.10180296430697913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG652.226523E-615.211192
CCAACGA1152.7284841E-1113.9790619
GGTATCA8200.013.504571
TTAGAAC1352.910383E-1112.6085653
AATCCCG3300.012.03544919
TCCAACG2250.011.76799418
CCCGTTT1251.960143E-811.3477112
CCGGTTT1850.011.24547812
TAGGACC3200.011.2295044
GCCGGTT1803.6379788E-1211.03249511
TAATACC1201.3587123E-711.0324944
CCGTTTC1658.731149E-1110.88921513
AGTCGTC700.001549739710.8073428
GTATTAG1302.216675E-710.6478331
AAGGCGG803.9344025E-410.6384766
AAGTCGT803.9344025E-410.6384767
GATATAC6250.010.5991571
GTATCAA17750.010.4721561
GGCCTAC1251.5401602E-610.2827651
GGGTAAG1459.018186E-810.2282141
Produced by FastQC (version 0.11.5)