FastQCFastQC Report
Thu 26 May 2016
SRR1511698_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511698_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1597203
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56480.3536181687612658No Hit
TATCAACGCAGAGTACTTTTTTTTT38980.2440516327605195No Hit
GGTATCAACGCAGAGTACTTTTTTT35700.22351573344152245No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17440.10919087930588661No Hit
TTGTAGAACAGTGTATATCAATGAG16470.10311776273898811No Hit
CTGTAGGACGTGGAATATGGCAAGA16370.10249166824755525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.013.2714181
CGTGCGC550.003075106512.08744210
TCTTATA1501.7644197E-1011.4006582
CTCTTAT1158.3950545E-710.77992251
TCCAACG2800.010.51484218
CCTAGGA1553.6561687E-910.4199552
CCGTGCT750.00265394510.1304289
GTCCTAA4900.010.1199261
ATAGGAC2002.5465852E-119.9755753
TAGGACT2002.5465852E-119.9755754
CCAACGA1856.511982E-109.7539619
GTGGTAT3800.09.5360841
TAAGCTG1401.057253E-69.5005485
ACCGTGC1002.7628877E-49.4972768
GTTCTAA1951.5697879E-99.291571
GATATAC5750.09.2873171
GTATCAA21100.09.1745271
GGATTGG1151.06540996E-49.1214721
TAGGACC3750.09.1205264
ACTGTTC6250.09.1173868
Produced by FastQC (version 0.11.5)