FastQCFastQC Report
Thu 26 May 2016
SRR1511697_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511697_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348053
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60790.25889534861436264No Hit
TATCAACGCAGAGTACTTTTTTTTT37240.15859948646815042No Hit
GGTATCAACGCAGAGTACTTTTTTT36840.15689594740834215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.012.6528281
CACAACG751.5505237E-512.6088884
GTATCAA29700.011.0166411
TAGGACC3800.010.9498244
GTTCTAA2051.8189894E-1210.6083161
GTCCTAG2800.010.5910961
TCCAACG1606.657501E-910.04770818
GTCCTAA6550.09.9604811
CTCTTAT1802.1082087E-99.8850221
TTAGGAC7350.09.7783213
GGCGAGG5050.09.73755719
AGGACGT12000.09.6142775
GGACGTG12000.09.5354726
GCCCTAA1354.13387E-69.518911
ACCGCTC1002.885647E-49.4566678
TAGGACG12000.09.4566664
GTAGGAC11150.09.414263
GTATAAG2352.910383E-119.2540631
GTGGTAT5700.09.1913371
GCCGGTT1252.9073633E-59.078411
Produced by FastQC (version 0.11.5)