FastQCFastQC Report
Thu 26 May 2016
SRR1511697_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511697_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348053
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74280.3163472034063967No Hit
TATCAACGCAGAGTACTTTTTTTTT48520.20663928795474376No Hit
GGTATCAACGCAGAGTACTTTTTTT46030.19603475730743725No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT655.4708886E-513.1497186
TGTACCG1103.812238E-812.0920085
GGTATCA13850.011.9830711
CGTGCGC1353.7289283E-1011.959208510
TCCAACG1406.8394E-1011.53209418
GTATCAA31050.010.8438081
GTCCTAC11050.010.358281
GTATAAA3050.010.3200741
GCGTGCG1201.5306232E-610.2884389
GTCCTAA6750.010.1741331
CCGCCCG750.002655336410.1301549
TAGCACC1606.166374E-910.09466654
GGACCGA951.6498513E-49.9968626
ACTATCG2107.2759576E-129.94925811
GATATAC4250.09.8748931
AAGGCGT1651.0279109E-89.7848076
TAGGACC4600.09.7074034
CCCCGTA1201.707342E-59.4970215
ACCGTCC800.0045286089.4970198
GTACCGC1002.7648854E-49.4970186
Produced by FastQC (version 0.11.5)