FastQCFastQC Report
Thu 26 May 2016
SRR1511696_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511696_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522975
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60690.24054935146008183No Hit
GTCCTACAGTGGACATTTCTAAATT47850.18965705169492364No Hit
CTGTAGGACGTGGAATATGGCAAGA45120.17883649263270543No Hit
GTCCTAAAGTGTGTATTTCTCATTT40750.16151567098366015No Hit
CTTTAGGACGTGAAATATGGCGAGG38170.15128964813365176No Hit
GGTATCAACGCAGAGTACTTTTTTT37010.14669190142589603No Hit
TATCAACGCAGAGTACTTTTTTTTT36780.145780279233841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAGG602.9371568E-413.1744211
GGTATCA12400.012.5103871
CGTGTAA550.0031752512.0362649
TAGGACC6750.011.90889554
TCCAACG2550.011.86792418
GTATAGG1850.011.7501591
CCAACGA1701.8189894E-1211.68248719
GTCCTAG3750.011.0665141
GTATCAA26700.010.6949661
CCAACGT1159.350333E-710.69080519
CTAGGAC3700.010.4794493
GGCGAGG13400.010.44532219
CCGTTTC2001.8189894E-1210.40277113
TAGGACA8350.010.30654
CGTTTCC1856.548362E-1110.22385314
TTAGGAC18500.010.0704963
CGGTAGG907.7712693E-49.88081551
GACGTGA14900.09.8378857
AGGACGT27800.09.7972465
GGACGTG27600.09.6969186
Produced by FastQC (version 0.11.5)