##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511696_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2522975 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.237126011950178 31.0 30.0 34.0 23.0 34.0 2 26.602249725027 31.0 25.0 34.0 10.0 34.0 3 28.250227211922432 31.0 27.0 34.0 16.0 34.0 4 32.723217233623004 35.0 32.0 37.0 26.0 37.0 5 33.17303857549123 35.0 35.0 37.0 27.0 37.0 6 33.424559101853966 37.0 35.0 37.0 28.0 37.0 7 33.459549936087356 37.0 35.0 37.0 28.0 37.0 8 33.480191837017806 37.0 35.0 37.0 28.0 37.0 9 35.06008937861057 39.0 35.0 39.0 28.0 39.0 10 34.93534260149229 39.0 35.0 39.0 28.0 39.0 11 34.93812146374815 39.0 35.0 39.0 27.0 39.0 12 34.84622201963951 39.0 35.0 39.0 27.0 39.0 13 34.836574282345246 39.0 35.0 39.0 27.0 39.0 14 35.967987792189774 40.0 36.0 41.0 27.0 41.0 15 35.90938515046721 40.0 36.0 41.0 27.0 41.0 16 35.8519850573232 40.0 36.0 41.0 27.0 41.0 17 35.825706556743526 40.0 36.0 41.0 27.0 41.0 18 35.76704961404691 40.0 36.0 41.0 27.0 41.0 19 35.79492266074773 40.0 36.0 41.0 27.0 41.0 20 35.72408406742041 40.0 36.0 41.0 27.0 41.0 21 35.63598569149516 40.0 36.0 41.0 27.0 41.0 22 35.5317773660064 40.0 36.0 41.0 27.0 41.0 23 35.419851564125686 40.0 36.0 41.0 26.0 41.0 24 35.311860006539895 40.0 36.0 41.0 25.0 41.0 25 35.20036900880905 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2093628322998136 1101 2 3.66259357139295 1101 3 2.265436994521078 1101 4 1.3051984038877578 1101 5 0.8674477397252218 1101 6 0.7506500892863812 1101 7 0.5595268363814014 1101 8 0.23025195325991632 1101 9 0.39830906933662646 1101 10 0.5671571627474421 1101 11 0.35483656535397756 1101 12 0.46189315519383456 1101 13 0.5050050696512329 1101 14 0.7603392662933288 1101 15 0.6269072280732857 1101 16 0.721151659807937 1101 17 0.831066201285168 1101 18 0.8070906168510206 1101 19 0.9122675999799625 1101 20 0.8332371071100866 1101 21 0.855703755485834 1101 22 0.896284358335997 1101 23 0.8905496259695269 1101 24 0.9338359643326655 1101 25 0.9670345303489043 1102 1 1.39880150549007 1102 2 3.9733170993813687 1102 3 2.5334790560960094 1102 4 1.5799737624323384 1102 5 1.2228048899058805 1102 6 0.9204175120716513 1102 7 0.8448147178412526 1102 8 0.8623696321631016 1102 9 0.9262525798540651 1102 10 0.9081583807942195 1102 11 0.8064879004890884 1102 12 0.9251817584591819 1102 13 0.9381119501801223 1102 14 1.075073326080144 1102 15 1.011369339436392 1102 16 1.0321667808128865 1102 17 1.049043874528742 1102 18 1.0127270565454012 1102 19 0.9324428838832901 1102 20 1.0123295597313486 1102 21 1.0787482162438167 1102 22 1.0385368426182708 1102 23 1.021936476805955 1102 24 0.8647415431509557 1102 25 0.8073545444569206 1103 1 1.5520256323668917 1103 2 4.261060361038208 1103 3 2.8346185869983884 1103 4 1.8388615434411903 1103 5 1.5077934431777535 1103 6 1.2002163238919081 1103 7 1.1411953913556943 1103 8 0.9500114905768271 1103 9 1.1401069972428104 1103 10 0.8976641017666935 1103 11 1.0646759433168143 1103 12 1.2095973276038166 1103 13 1.078533519016645 1103 14 1.1652542515481628 1103 15 1.1451247611118518 1103 16 0.9515657513547282 1103 17 1.173533694444309 1103 18 1.1165019556952132 1103 19 1.3306399469759214 1103 20 1.1880304090034386 1103 21 1.3186870633917778 1103 22 1.3880739013382808 1103 23 1.1894716545436381 1103 24 1.4329361209872147 1103 25 1.0452360298151362 1104 1 1.4597969455025677 1104 2 4.076086000204732 1104 3 2.539404487616533 1104 4 1.7607982261156252 1104 5 1.130402884006358 1104 6 0.988695706885828 1104 7 0.8401750697708152 1104 8 0.928874219219729 1104 9 0.9837539758475273 1104 10 0.8795107388243935 1104 11 0.9487661231457949 1104 12 0.862379647686133 1104 13 0.9057026665529193 1104 14 0.9946008770358432 1104 15 1.1057325034613683 1104 16 0.930803734345254 1104 17 1.0014155552739297 1104 18 1.031147789639185 1104 19 1.2029663079574888 1104 20 1.0342640586354364 1104 21 1.072600044492539 1104 22 1.1346204429047404 1104 23 1.2082546194617905 1104 24 0.9831478909381701 1104 25 1.0727533964166511 1105 1 1.249603154710229 1105 2 3.807495124419848 1105 3 2.358941096966909 1105 4 1.4528172653662068 1105 5 0.9612813668815008 1105 6 0.7264650081951629 1105 7 0.5254001159536017 1105 8 0.6027385944141557 1105 9 0.6544375253394037 1105 10 0.5210504796110556 1105 11 0.5672275544362719 1105 12 0.6773372317624009 1105 13 0.5587775998772102 1105 14 0.6169238970731996 1105 15 0.6463271004068929 1105 16 0.6124329363155212 1105 17 0.6452214961570135 1105 18 0.7430480952638305 1105 19 0.5989079724249748 1105 20 0.7984871041783705 1105 21 0.7658744594491225 1105 22 0.7449193520621264 1105 23 0.7560619137622524 1105 24 0.7828389859530844 1105 25 0.8346588252715677 1106 1 0.9245994903753001 1106 2 3.4292582349347427 1106 3 1.9238841721181288 1106 4 1.26988064151179 1106 5 0.7518068955638881 1106 6 0.5428000654714538 1106 7 0.3921537343422159 1106 8 0.36162556275740343 1106 9 0.3496004171725815 1106 10 0.28247550380393704 1106 11 0.38255313653836254 1106 12 0.2785567058884766 1106 13 0.5119481230367384 1106 14 0.48130463894132447 1106 15 0.6327214093071234 1106 16 0.3600583542512652 1106 17 0.3198996703858512 1106 18 0.5758024740148642 1106 19 0.4565041645762875 1106 20 0.5163670119839097 1106 21 0.3729275134371335 1106 22 0.4291202489920849 1106 23 0.3195648897810699 1106 24 0.339831539652387 1106 25 0.24387417797698419 1107 1 1.5891809023482928 1107 2 4.0264966614008095 1107 3 2.5954787808273068 1107 4 1.6233664984477585 1107 5 1.3284557468218878 1107 6 1.180541693896103 1107 7 0.9098135822630766 1107 8 0.920134920476734 1107 9 0.9820011268258639 1107 10 1.0575661549737916 1107 11 0.9640373341597623 1107 12 1.0388542713628794 1107 13 1.090560002691987 1107 14 1.1528492459837807 1107 15 1.1989310237016753 1107 16 1.310908567776906 1107 17 1.1481328337135182 1107 18 1.11852426844505 1107 19 1.284568803767968 1107 20 1.236724357228752 1107 21 1.3127829160246236 1107 22 1.2983195926739128 1107 23 1.515614017344582 1107 24 1.5229061606582732 1107 25 1.4414900244311255 1108 1 1.4840043356938004 1108 2 4.047419579585078 1108 3 2.54325640390201 1108 4 1.484038555507631 1108 5 1.0421518117810393 1108 6 1.0034725907232769 1108 7 0.7669550461173742 1108 8 0.7152969517684582 1108 9 0.7076103374433416 1108 10 0.6071723683873529 1108 11 0.6994298326749515 1108 12 0.7070397186464632 1108 13 0.780643670150333 1108 14 0.8171832593380159 1108 15 0.8431048722904109 1108 16 0.8723986677890778 1108 17 0.9352281086107936 1108 18 0.9153587744949476 1108 19 0.7646834051149582 1108 20 0.8505959092839817 1108 21 0.8892893035800427 1108 22 0.9491443282157377 1108 23 1.0168281001672241 1108 24 1.0451026887177122 1108 25 1.0256289908397065 1109 1 1.0443151408650024 1109 2 3.1640546456902463 1109 3 2.003483804565363 1109 4 1.2451421661763789 1109 5 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-2.11167253539805 2315 21 -2.174351232043513 2315 22 -2.110453437203738 2315 23 -2.1427951327125285 2315 24 -2.0637122044513347 2315 25 -2.0630270206786463 2316 1 -0.5404245921799351 2316 2 1.6886192984815551 2316 3 0.39183766925471275 2316 4 -0.6797945102615657 2316 5 -0.8509192442269011 2316 6 -0.9370572507369133 2316 7 -1.4063652636122868 2316 8 -1.2062648490993553 2316 9 -1.5493511655578942 2316 10 -1.5706127163627812 2316 11 -1.5597688933329295 2316 12 -1.6244690207073873 2316 13 -1.547612391950338 2316 14 -1.7901518663526517 2316 15 -1.8782029038325518 2316 16 -1.9559702225612057 2316 17 -1.7617925808665191 2316 18 -1.9444763028407621 2316 19 -1.8386156929037796 2316 20 -1.7785698708750104 2316 21 -2.0637000003018997 2316 22 -1.800364062982375 2316 23 -2.128942774670783 2316 24 -1.8540159614695852 2316 25 -1.8359114048973169 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93342.0 3 1.0 4 854.0 5 4199.0 6 4829.0 7 4425.0 8 4003.0 9 4002.0 10 3841.0 11 3664.0 12 3626.0 13 3522.0 14 3616.0 15 3681.0 16 3893.0 17 4220.0 18 4934.0 19 6024.0 20 7786.0 21 10143.0 22 12585.0 23 14946.0 24 17276.0 25 19742.0 26 22077.0 27 25368.0 28 29097.0 29 35375.0 30 44229.0 31 55597.0 32 71289.0 33 94423.0 34 129386.0 35 184951.0 36 294844.0 37 374669.0 38 922516.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.278778029905176 19.262339103637572 13.318364232701473 25.140518633755782 2 16.02665324893414 21.323800117198097 40.43359154185897 22.215955092008787 3 18.40604841506555 25.12918280997632 29.981430652305313 26.48333812265282 4 12.036583794924642 17.413252212170153 39.122385279283385 31.42777871362182 5 12.922482386864715 37.95027695478552 36.231036772064726 12.89620388628504 6 30.724283831587705 37.74912553632121 18.798759401103858 12.727831230987228 7 27.146127092024297 31.803485963990923 23.101695419098483 17.948691524886296 8 24.1870410923612 37.67167728574402 21.296643843082073 16.84463777881271 9 26.055509864346654 15.716683677008294 20.845470129509806 37.38233632913525 10 14.807399994054638 29.339648629098587 34.85864901554712 20.994302361299656 11 33.65744805239846 22.731973166598955 25.334694160663503 18.275884620339085 12 22.99503562262805 24.812176101626058 32.4349230571052 19.757865218640692 13 28.682805021849205 22.370098792100595 26.146196454582388 22.800899731467812 14 20.55799205303263 22.708231353858043 28.134999355919106 28.59877723719022 15 22.48817368384546 31.42357732438887 23.8590552819588 22.229193709806875 16 21.620705714483893 28.39520803812959 28.472735560201745 21.511350687184773 17 21.238815287507805 28.769250587104512 28.493940685103897 21.497993440283793 18 21.03025198426461 27.77185663750136 30.502799274665822 20.695092103568207 19 23.093688998107393 27.120839485131643 27.917795459725127 21.86767605703584 20 22.99388618595111 27.797382058878906 28.68316174357653 20.525570011593455 21 22.563486684344245 27.139839157817967 27.68189871461016 22.614775443227625 22 22.609800634889428 28.465781647660542 27.916545611720206 21.00787210572982 23 22.01895152643935 28.005325484361048 28.220112621548555 21.75561036765105 24 22.626797829386206 27.09612825820966 28.941094494471404 21.335979417932734 25 22.416829338380285 27.60340471070859 28.539561430454125 21.440204520457 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 287.0 1 287.0 2 491.0 3 695.0 4 695.0 5 695.0 6 1828.0 7 2961.0 8 2961.0 9 2961.0 10 4197.0 11 5433.0 12 5433.0 13 5433.0 14 9074.5 15 12716.0 16 12716.0 17 12716.0 18 22833.5 19 32951.0 20 32951.0 21 32951.0 22 52215.0 23 71479.0 24 71479.0 25 71479.0 26 102615.0 27 133751.0 28 133751.0 29 133751.0 30 171944.5 31 210138.0 32 210138.0 33 210138.0 34 234645.0 35 259152.0 36 259152.0 37 259152.0 38 275379.5 39 291607.0 40 291607.0 41 291607.0 42 304460.0 43 317313.0 44 317313.0 45 317313.0 46 320094.0 47 322875.0 48 322875.0 49 322875.0 50 307899.5 51 292924.0 52 292924.0 53 292924.0 54 258885.0 55 224846.0 56 224846.0 57 224846.0 58 190902.0 59 156958.0 60 156958.0 61 156958.0 62 127203.0 63 97448.0 64 97448.0 65 97448.0 66 74543.0 67 51638.0 68 51638.0 69 51638.0 70 37767.0 71 23896.0 72 23896.0 73 23896.0 74 16619.5 75 9343.0 76 9343.0 77 9343.0 78 6289.0 79 3235.0 80 3235.0 81 3235.0 82 2100.0 83 965.0 84 965.0 85 965.0 86 617.5 87 270.0 88 270.0 89 270.0 90 168.5 91 67.0 92 67.0 93 67.0 94 43.5 95 20.0 96 20.0 97 20.0 98 13.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.290966022255472 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.98178737403264E-4 22 6.341719596904448E-4 23 4.756289697678336E-4 24 7.92714949613056E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2522975.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.60598109408699 #Duplication Level Percentage of deduplicated Percentage of total 1 82.83645010629569 59.31585280212689 2 12.012004436405247 17.20262725150646 3 2.9174648384079274 6.267237961851048 4 0.9657520671496814 2.7661449704758203 5 0.43851947020634735 1.5700308446492377 6 0.22963270926535076 0.9865845262943207 7 0.12858340995360307 0.6445138855505657 8 0.09078648154397902 0.5200684064829472 9 0.062696043155199 0.40404705127607166 >10 0.2780772279154598 3.4772693879159933 >50 0.01981823322516184 0.9819990592675246 >100 0.016795378857460182 2.453917702340237 >500 0.001906005230200066 1.005626880422398 >1k 0.0014575334113142017 2.1604600163982206 >5k 5.605897735823853E-5 0.24361925344235152 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6069 0.24054935146008183 No Hit GTCCTACAGTGGACATTTCTAAATT 4785 0.18965705169492364 No Hit CTGTAGGACGTGGAATATGGCAAGA 4512 0.17883649263270543 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4075 0.16151567098366015 No Hit CTTTAGGACGTGAAATATGGCGAGG 3817 0.15128964813365176 No Hit GGTATCAACGCAGAGTACTTTTTTT 3701 0.14669190142589603 No Hit TATCAACGCAGAGTACTTTTTTTTT 3678 0.145780279233841 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.96357474806528E-5 0.0 5 0.0 3.96357474806528E-5 0.0 3.96357474806528E-5 0.0 6 0.0 3.96357474806528E-5 0.0 3.96357474806528E-5 0.0 7 0.0 3.96357474806528E-5 0.0 3.96357474806528E-5 0.0 8 0.0 3.96357474806528E-5 0.0 3.96357474806528E-5 0.0 9 0.0 3.96357474806528E-5 0.0 7.92714949613056E-5 0.0 10 0.0 3.96357474806528E-5 0.0 7.92714949613056E-5 0.0 11 0.0 3.96357474806528E-5 0.0 1.189072424419584E-4 0.0 12 0.0 3.96357474806528E-5 0.0 1.189072424419584E-4 3.96357474806528E-5 13 0.0 3.96357474806528E-5 0.0 1.189072424419584E-4 3.96357474806528E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTAGG 60 2.9371568E-4 13.174421 1 GGTATCA 1240 0.0 12.510387 1 CGTGTAA 55 0.00317525 12.036264 9 TAGGACC 675 0.0 11.9088955 4 TCCAACG 255 0.0 11.867924 18 GTATAGG 185 0.0 11.750159 1 CCAACGA 170 1.8189894E-12 11.682487 19 GTCCTAG 375 0.0 11.066514 1 GTATCAA 2670 0.0 10.694966 1 CCAACGT 115 9.350333E-7 10.690805 19 CTAGGAC 370 0.0 10.479449 3 GGCGAGG 1340 0.0 10.445322 19 CCGTTTC 200 1.8189894E-12 10.402771 13 TAGGACA 835 0.0 10.3065 4 CGTTTCC 185 6.548362E-11 10.223853 14 TTAGGAC 1850 0.0 10.070496 3 CGGTAGG 90 7.7712693E-4 9.8808155 1 GACGTGA 1490 0.0 9.837885 7 AGGACGT 2780 0.0 9.797246 5 GGACGTG 2760 0.0 9.696918 6 >>END_MODULE