FastQCFastQC Report
Thu 26 May 2016
SRR1511696_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511696_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522975
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74810.2965150269027636No Hit
GTCCTACAGTGGACATTTCTAAATT52080.20642297287923977No Hit
CTGTAGGACGTGGAATATGGCAAGA50630.20067578949454512No Hit
GTCCTAAAGTGTGTATTTCTCATTT46950.1860898344216649No Hit
TATCAACGCAGAGTACTTTTTTTTT46490.18426659003755486No Hit
GGTATCAACGCAGAGTACTTTTTTT45050.17855904240034087No Hit
CTTTAGGACGTGAAATATGGCGAGG43270.17150387934878467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7250.012.5798364
TCCAACG2500.012.1561718
GGCGAGG11550.012.087119
GCCGGTT2100.011.758211
CGGTTTC1950.011.68862413
GGTATCA14150.011.66435051
ATGCGCT1001.9382896E-611.39640911
CCGGTTT2200.011.22373612
CCCCCGG909.550001E-510.55223118
TAATACT3250.010.5235164
CCCGCCC1553.6852725E-910.41607314
GGACGAG1201.530816E-610.2884256
CCAACGT1856.002665E-1110.26703519
TGGCGAG25400.010.24480318
AGGACGT28750.010.2439065
TTGGACT2700.010.2041294
GTATCAA28150.010.1674891
GGACGTG28600.010.1611346
AGGACGA1502.4450856E-810.1337575
CAGTCCG750.002655479410.1301429
Produced by FastQC (version 0.11.5)