FastQCFastQC Report
Thu 26 May 2016
SRR1511695_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511695_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2825330
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79280.280604389575731No Hit
CTGTAGGACGTGGAATATGGCAAGA70540.24966995005893117No Hit
GTCCTAAAGTGTGTATTTCTCATTT69160.24478556487206807No Hit
GTATCAACGCAGAGTACTTTTTTTT61860.21894787511547326No Hit
CTTTAGGACGTGAAATATGGCGAGG60120.21278930248855885No Hit
TATCAACGCAGAGTACTTTTTTTTT35360.1251535218894784No Hit
GGTATCAACGCAGAGTACTTTTTTT35290.12490576322057953No Hit
CTGAAGGACCTGGAATATGGCGAGA34230.12115398909153974No Hit
GTCCTACAGTGTGCATTTCTCATTT31560.11170376557782631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4600.013.97961818
CCAACGA3450.013.43139719
CTATGCG500.001552687713.2392879
TAGGACC12400.012.5834234
GGTATCA14000.012.3560921
CGGTAGG1102.9738112E-711.6821231
ACTGTTC11750.011.6698888
ACCGTGC752.1650117E-411.34795958
GGCGAGG18500.011.24592519
CGTTTCC3900.011.15397614
ATCCCGT3700.010.99014210
AGCACCG951.4319625E-510.9497865
GGCCTAC1903.6379788E-1210.9253861
CCGTTTC3900.010.91149913
CGTTAGG1001.5473925E-510.8733611
GATATAC11000.010.7834991
CCAACGT1701.5097612E-1010.56936519
AGGACGT44000.010.5527435
CCACCTT11850.010.53397113
CACCTTT11950.010.52495614
Produced by FastQC (version 0.11.5)