FastQCFastQC Report
Thu 26 May 2016
SRR1511695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2825330
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT88660.31380405120817745No Hit
CTGTAGGACGTGGAATATGGCAAGA79400.2810291187224147No Hit
GTCCTAAAGTGTGTATTTCTCATTT77380.2738795114199049No Hit
GTATCAACGCAGAGTACTTTTTTTT72470.25650101050142815No Hit
CTTTAGGACGTGAAATATGGCGAGG66770.23632637603395001No Hit
TATCAACGCAGAGTACTTTTTTTTT45230.16008749420421686No Hit
GGTATCAACGCAGAGTACTTTTTTT44960.15913185362417842No Hit
CTGAAGGACCTGGAATATGGCGAGA37220.13173682366307654No Hit
GTCCTACAGTGTGCATTTCTCATTT36660.1297547543118857No Hit
TTTCTAAATTTTCCACCTTTTTCAG29950.10600531619315266No Hit
GTCCTTCAGTGTGCATTTCTCATTT28870.10218275387299891No Hit
GATATACACTGTTCTACAAATCCCG28530.1009793546240616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2800.012.54972419
TAGGACC10700.012.4307184
GGTATCA15150.012.2142481
TCCAACG3450.011.83695118
GCGACTA658.0476503E-411.688724516
CTAGGAC5650.011.6025273
GGCGTGC1104.9928167E-711.2238338
CGTATTC600.00587482111.0840573
CTACACG600.00587482111.0840574
CGTGCGC600.005891097711.07993810
CGGAGTA600.005891097711.07993814
GGCGAGG17950.011.05789119
CGACTAA700.001495876110.85381517
GAACAGT8500.010.8378546
AGGACCT23000.010.4093755
GTCCTAT4600.010.3678761
GGACCTG22200.010.3526836
TAGGACT5450.010.2850744
GGAGCGC750.002655460710.1302288
GCGAAGT750.002655460710.13022813
Produced by FastQC (version 0.11.5)