FastQCFastQC Report
Thu 26 May 2016
SRR1511694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124801
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT73990.3482208451520872No Hit
TATCAACGCAGAGTACTTTTTTTTT46280.21780863243193127No Hit
GGTATCAACGCAGAGTACTTTTTTT44000.20707821579526742No Hit
GTCCTACAGTGGACATTTCTAAATT27220.12810611440789044No Hit
GTCCTAAAGTGTGTATTTCTCATTT24360.11464603038119806No Hit
CTGTAGGACGTGGAATATGGCAAGA24320.11445777745774781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTCA400.005441484514.18540610
GGTATCA14300.012.6468441
TAGGACC6100.012.5575724
TCCAACG1950.011.15433618
CGGTAGG1001.5509793E-510.8707561
TTAGGAC10750.010.6445533
CGGTTTC1605.8935257E-1010.63905413
GTATCAA28950.010.5823031
CGATATT750.001907688310.541341
TAGATAG1356.081973E-810.5077085
CTAGGAC2100.010.3575973
TGGCGAG15850.010.26241818
AGGACCT13000.010.2571395
GGACCTG12200.010.2320976
AAATCCG750.002749943210.08739955
TAGGACA6600.010.0300854
ACTGCGC856.87628E-410.0132278
CCAACGT1054.316252E-59.90726719
GGCGAGG7650.09.88960719
CTCAACG800.00325944079.8825052
Produced by FastQC (version 0.11.5)