##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511694_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2124801 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.064196600058075 31.0 28.0 34.0 22.0 34.0 2 26.436948213032657 31.0 25.0 34.0 10.0 34.0 3 28.076634941342743 31.0 27.0 34.0 16.0 34.0 4 32.532127008599865 35.0 32.0 37.0 25.0 37.0 5 32.97633896068385 35.0 33.0 37.0 26.0 37.0 6 33.222463656596545 37.0 35.0 37.0 27.0 37.0 7 33.251588266383536 37.0 35.0 37.0 27.0 37.0 8 33.266123274603125 37.0 35.0 37.0 27.0 37.0 9 34.828907742419176 39.0 35.0 39.0 27.0 39.0 10 34.69753779295096 39.0 35.0 39.0 27.0 39.0 11 34.705001550733456 39.0 35.0 39.0 27.0 39.0 12 34.60287292786477 39.0 35.0 39.0 27.0 39.0 13 34.59263008629985 39.0 35.0 39.0 27.0 39.0 14 35.70132450050617 40.0 36.0 41.0 27.0 41.0 15 35.62978603643353 40.0 36.0 41.0 27.0 41.0 16 35.56808896456656 40.0 36.0 41.0 27.0 41.0 17 35.53893564620875 40.0 36.0 41.0 27.0 41.0 18 35.47898885589756 40.0 36.0 41.0 26.0 41.0 19 35.50671898215409 40.0 36.0 41.0 26.0 41.0 20 35.431906799742656 40.0 36.0 41.0 25.0 41.0 21 35.34753701640766 40.0 36.0 41.0 25.0 41.0 22 35.23425017213377 40.0 36.0 41.0 25.0 41.0 23 35.11722415416785 40.0 36.0 41.0 24.0 41.0 24 34.995225435228996 40.0 35.0 41.0 24.0 41.0 25 34.87622323219916 40.0 35.0 41.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2261364928873029 1101 2 3.7225572635381923 1101 3 2.3152727221908407 1101 4 1.3003714213299205 1101 5 0.8663291703811069 1101 6 0.6789513170977273 1101 7 0.6238354466762601 1101 8 0.4962461816837802 1101 9 0.5074715334265676 1101 10 0.6632740439958269 1101 11 0.5667687421386418 1101 12 0.48998094919545565 1101 13 0.5699465403685693 1101 14 0.7460690810275779 1101 15 0.6919689991663773 1101 16 0.6436258849245249 1101 17 0.6595502249977301 1101 18 0.6627499976984126 1101 19 0.8002498056305427 1101 20 0.8169665135094988 1101 21 0.7664920834181785 1101 22 0.9377366707195307 1101 23 0.7919527940530955 1101 24 0.7426482050147669 1101 25 0.5215846793695817 1102 1 1.4932563120317468 1102 2 4.057712231627665 1102 3 2.6450212623295677 1102 4 1.6492753412314016 1102 5 1.1777067905587089 1102 6 0.8540259036409239 1102 7 0.917644734352848 1102 8 0.8337747035077214 1102 9 0.9706112789610231 1102 10 0.9520058330085988 1102 11 0.94174520542078 1102 12 1.1193530772773101 1102 13 1.120663343070639 1102 14 1.126347299651819 1102 15 1.1656222018558182 1102 16 1.104086245583055 1102 17 1.3078748200199968 1102 18 1.074352810175391 1102 19 1.1838661448493184 1102 20 1.0123157165357668 1102 21 1.081770073984778 1102 22 0.7366632209559469 1102 23 0.8452557119043931 1102 24 0.8578547375211656 1102 25 0.6583441206634504 1103 1 1.3535798970180082 1103 2 3.941160951261235 1103 3 2.5400473090447697 1103 4 1.5561231025593045 1103 5 1.1150331682614834 1103 6 0.8970724539306829 1103 7 0.8103444161381432 1103 8 0.586705154108941 1103 9 0.6547797071007295 1103 10 0.5542439800004573 1103 11 0.7037835620579713 1103 12 0.7874841768537308 1103 13 0.7921613705141368 1103 14 0.8203880189856534 1103 15 0.7243429055102553 1103 16 0.7582089156346328 1103 17 0.989020052348522 1103 18 0.7477576848125338 1103 19 0.8956571761959253 1103 20 0.7857250474844548 1103 21 0.8583998705867941 1103 22 0.8832409581885443 1103 23 0.7459492218401209 1103 24 0.984868415559184 1103 25 0.8484163100720181 1104 1 1.303802926192791 1104 2 3.7298475456797497 1104 3 2.292513243161217 1104 4 1.556093279606408 1104 5 0.9599921179244433 1104 6 0.7997568753777102 1104 7 0.641101073701968 1104 8 0.6318067614566587 1104 9 0.5947844076358777 1104 10 0.6545221542775863 1104 11 0.9324834755714093 1104 12 0.7722107208960765 1104 13 0.8033936795521655 1104 14 0.8894207675975849 1104 15 0.9679138019198135 1104 16 0.7943622690888787 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1107 17 1.080056760129132 1107 18 0.930015667192734 1107 19 1.0794458506909166 1107 20 1.1753876350896988 1107 21 1.1994510608002784 1107 22 1.1652353468183634 1107 23 1.2631733476602705 1107 24 1.2715481069850227 1107 25 1.2278770677982962 1108 1 1.3185286840416843 1108 2 3.844080959100321 1108 3 2.358486214456885 1108 4 1.41757314007927 1108 5 0.8347260224088373 1108 6 0.7532658883936065 1108 7 0.7024887304163414 1108 8 0.6554260939053904 1108 9 0.64878615911325 1108 10 0.37146843672252317 1108 11 0.5176266524440294 1108 12 0.5063065576402579 1108 13 0.6377603648369004 1108 14 0.6627632917542812 1108 15 0.7457970656570438 1108 16 0.6810289751588599 1108 17 0.7003150851924502 1108 18 0.655165618736774 1108 19 0.49811308388969877 1108 20 0.6706123708131386 1108 21 0.5895797237359872 1108 22 0.5892882729470372 1108 23 0.5496972074901336 1108 24 0.7273915850265666 1108 25 0.7998040918652407 1109 1 1.1545108299331126 1109 2 3.226856358993359 1109 3 2.076584649941882 1109 4 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-0.974489473121082 2314 10 -0.8149453288262336 2314 11 -0.8722342608034452 2314 12 -0.9930056892758401 2314 13 -1.103576900413131 2314 14 -1.119858175595077 2314 15 -1.3279712378065724 2314 16 -1.2074907734170708 2314 17 -1.2395608940017695 2314 18 -1.0996091416666687 2314 19 -1.096641012533837 2314 20 -1.1356572786155752 2314 21 -1.143515582409222 2314 22 -1.1198379612235598 2314 23 -1.1232578302596679 2314 24 -1.0193265864992398 2314 25 -1.0007115106212368 2315 1 -1.7683568248373156 2315 2 -3.718898940679125 2315 3 -1.7934696401585697 2315 4 -1.4874270253724085 2315 5 -1.5287252707758476 2315 6 -1.6235704453699 2315 7 -1.467908152186343 2315 8 -1.5854801784395782 2315 9 -1.6706427592500148 2315 10 -1.7832475834597048 2315 11 -1.5690196708600581 2315 12 -1.7677328515102246 2315 13 -1.579329498442469 2315 14 -1.8621303515923273 2315 15 -1.7936534477466992 2315 16 -2.0325101512850665 2315 17 -1.9456546558540921 2315 18 -1.9170936023167329 2315 19 -1.8966431050820134 2315 20 -1.8877805610912688 2315 21 -1.9439069761073426 2315 22 -1.9590469954515441 2315 23 -1.955420644457952 2315 24 -1.9081586568925601 2315 25 -1.6478394446571372 2316 1 -0.5213814358449866 2316 2 1.819725402304183 2316 3 0.43903847471842994 2316 4 -0.5166468987199124 2316 5 -0.6910140230894797 2316 6 -0.8874816238532546 2316 7 -1.2030185200058412 2316 8 -1.321930118848094 2316 9 -1.4526164730900675 2316 10 -1.509200360642481 2316 11 -1.4588649526975601 2316 12 -1.5892552628507346 2316 13 -1.6198368773509273 2316 14 -1.6832765402089862 2316 15 -1.6272375046908962 2316 16 -1.830780350600726 2316 17 -1.7701563667063382 2316 18 -1.9467366575747889 2316 19 -1.8965206267354162 2316 20 -1.7085002724158471 2316 21 -1.9639931503341614 2316 22 -1.8465451417994387 2316 23 -1.901456300486032 2316 24 -2.160730702171101 2316 25 -1.8654315643014598 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 86420.0 3 4.0 4 744.0 5 3771.0 6 4520.0 7 4109.0 8 3710.0 9 3568.0 10 3458.0 11 3389.0 12 3299.0 13 3229.0 14 3309.0 15 3370.0 16 3543.0 17 3929.0 18 4409.0 19 5450.0 20 7143.0 21 9057.0 22 11213.0 23 13366.0 24 15420.0 25 17401.0 26 19505.0 27 21897.0 28 25553.0 29 30573.0 30 38131.0 31 48542.0 32 61477.0 33 80599.0 34 109839.0 35 155063.0 36 245105.0 37 313099.0 38 757587.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.549960678670615 18.502250328383692 12.98785156821745 24.959937424728246 2 16.66451904822797 20.942279885504067 39.550721501431205 22.842479564836765 3 18.8953224325478 24.586584814295552 29.41080129386234 27.10729145929431 4 12.366616920831646 16.840824152473573 37.95786052435028 32.8346984023445 5 13.422103999386295 37.52916155442321 35.414940034384394 13.633794411806093 6 31.654352572311478 36.921763496911005 18.234036975697958 13.18984695507956 7 28.052838830554016 31.560884995818434 22.057595040664985 18.32868113296257 8 25.301287038174397 36.60060400950489 20.513309246371776 17.584799705948935 9 26.581830486713816 15.425350421051196 20.033687860651423 37.95913123158357 10 15.423138449200655 28.816628004222512 33.83017986154939 21.930053685027445 11 34.470286864511074 22.523521026204335 24.3055702628152 18.700621846469385 12 23.562065341648463 24.482669200551015 31.478853784424988 20.47641167337553 13 29.223113129182448 21.781757444579515 25.708995807136763 23.28613361910127 14 21.412169892615825 21.904592477130798 27.483891432656517 29.19934619759686 15 23.297099351892246 30.190262523408073 23.61458790729108 22.898050217408596 16 22.474104633798646 27.884446590527773 27.25671721728294 22.384731558390644 17 21.961303670320184 28.0507209851652 27.597549135189603 22.390426209325014 18 21.764861744699857 27.167720647721833 29.507704486208358 21.559713121369956 19 23.51284826807229 26.64081325301205 27.634083207831324 22.212255271084338 20 23.638025396260637 26.719866942833704 28.24203301862151 21.400074642284146 21 23.45161606102421 26.370296254322767 27.31081007306113 22.867277611591888 22 23.45865291528868 27.317827107828997 27.49318635661746 21.730333620264865 23 22.960089646632813 26.922369159826374 27.698964649162484 22.41857654437833 24 23.306193524096386 26.432605421686745 28.199595256024097 22.061605798192772 25 23.029262505053413 26.832771633672987 28.05961593579822 22.078349925475376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 379.0 1 379.0 2 555.5 3 732.0 4 732.0 5 732.0 6 1859.0 7 2986.0 8 2986.0 9 2986.0 10 4016.5 11 5047.0 12 5047.0 13 5047.0 14 7369.5 15 9692.0 16 9692.0 17 9692.0 18 16926.0 19 24160.0 20 24160.0 21 24160.0 22 37985.5 23 51811.0 24 51811.0 25 51811.0 26 74864.0 27 97917.0 28 97917.0 29 97917.0 30 126261.0 31 154605.0 32 154605.0 33 154605.0 34 173290.0 35 191975.0 36 191975.0 37 191975.0 38 207369.0 39 222763.0 40 222763.0 41 222763.0 42 238667.0 43 254571.0 44 254571.0 45 254571.0 46 264493.5 47 274416.0 48 274416.0 49 274416.0 50 268418.0 51 262420.0 52 262420.0 53 262420.0 54 237819.0 55 213218.0 56 213218.0 57 213218.0 58 184511.5 59 155805.0 60 155805.0 61 155805.0 62 129273.5 63 102742.0 64 102742.0 65 102742.0 66 79751.5 67 56761.0 68 56761.0 69 56761.0 70 41719.0 71 26677.0 72 26677.0 73 26677.0 74 18598.0 75 10519.0 76 10519.0 77 10519.0 78 7176.0 79 3833.0 80 3833.0 81 3833.0 82 2549.0 83 1265.0 84 1265.0 85 1265.0 86 816.5 87 368.0 88 368.0 89 368.0 90 240.5 91 113.0 92 113.0 93 113.0 94 66.0 95 19.0 96 19.0 97 19.0 98 13.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.3082152163896765 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.706323086256078E-5 20 0.0 21 2.353161543128039E-4 22 9.412646172512156E-4 23 6.588852320758509E-4 24 4.706323086256078E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2124801.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.3219055604563 #Duplication Level Percentage of deduplicated Percentage of total 1 79.75897947993087 54.49285463626208 2 13.310188462533945 18.187548782582383 3 3.8604759710487944 7.912652241372195 4 1.3808300334031174 3.7736375654883796 5 0.6208720421667908 2.120958051502356 6 0.32010410293312624 1.312207337406698 7 0.18124805848753095 0.8668248914500803 8 0.12137232239364552 0.6633910678585524 9 0.07482627816756912 0.46010465193645816 >10 0.3278885128812576 3.8426744992188246 >50 0.022758125577630895 1.069826311223964 >100 0.016968020998126228 2.3201265770965405 >500 0.002302469055201994 1.0653637818775323 >1k 0.0011163486328250396 1.5591235945171762 >5k 6.977178955156498E-5 0.3527060102067627 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7399 0.3482208451520872 No Hit TATCAACGCAGAGTACTTTTTTTTT 4628 0.21780863243193127 No Hit GGTATCAACGCAGAGTACTTTTTTT 4400 0.20707821579526742 No Hit GTCCTACAGTGGACATTTCTAAATT 2722 0.12810611440789044 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2436 0.11464603038119806 No Hit CTGTAGGACGTGGAATATGGCAAGA 2432 0.11445777745774781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.706323086256078E-5 2 0.0 0.0 0.0 0.0 4.706323086256078E-5 3 0.0 0.0 0.0 0.0 4.706323086256078E-5 4 0.0 0.0 0.0 0.0 4.706323086256078E-5 5 0.0 0.0 0.0 0.0 4.706323086256078E-5 6 0.0 0.0 0.0 0.0 4.706323086256078E-5 7 4.706323086256078E-5 0.0 0.0 0.0 4.706323086256078E-5 8 4.706323086256078E-5 0.0 0.0 0.0 9.412646172512156E-5 9 4.706323086256078E-5 0.0 0.0 0.0 9.412646172512156E-5 10 4.706323086256078E-5 0.0 0.0 0.0 9.412646172512156E-5 11 4.706323086256078E-5 0.0 0.0 0.0 9.412646172512156E-5 12 4.706323086256078E-5 0.0 0.0 0.0 1.882529234502431E-4 13 4.706323086256078E-5 0.0 0.0 0.0 1.882529234502431E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGTCA 40 0.0054414845 14.185406 10 GGTATCA 1430 0.0 12.646844 1 TAGGACC 610 0.0 12.557572 4 TCCAACG 195 0.0 11.154336 18 CGGTAGG 100 1.5509793E-5 10.870756 1 TTAGGAC 1075 0.0 10.644553 3 CGGTTTC 160 5.8935257E-10 10.639054 13 GTATCAA 2895 0.0 10.582303 1 CGATATT 75 0.0019076883 10.54134 1 TAGATAG 135 6.081973E-8 10.507708 5 CTAGGAC 210 0.0 10.357597 3 TGGCGAG 1585 0.0 10.262418 18 AGGACCT 1300 0.0 10.257139 5 GGACCTG 1220 0.0 10.232097 6 AAATCCG 75 0.0027499432 10.0873995 5 TAGGACA 660 0.0 10.030085 4 ACTGCGC 85 6.87628E-4 10.013227 8 CCAACGT 105 4.316252E-5 9.907267 19 GGCGAGG 765 0.0 9.889607 19 CTCAACG 80 0.0032594407 9.882505 2 >>END_MODULE