FastQCFastQC Report
Thu 26 May 2016
SRR1511694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124801
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT88900.4183921223681653No Hit
TATCAACGCAGAGTACTTTTTTTTT56660.2666602660672694No Hit
GGTATCAACGCAGAGTACTTTTTTT55410.26077736220944925No Hit
GTCCTACAGTGGACATTTCTAAATT32250.1517789195317585No Hit
CTGTAGGACGTGGAATATGGCAAGA27670.13022395979670567No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27190.12796492471530274No Hit
GTCCTAAAGTGTGTATTTCTCATTT26660.12547057347958704No Hit
CTTTAGGACGTGAAATATGGCGAGG23810.1120575526837572No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22180.10438624605315981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16600.013.4467081
TAGGACC5850.011.8558764
TAGGTCG658.0188765E-411.6934675
ATTATAC2200.010.7965253
GCTATAT1951.8189894E-1210.7620811
CGGTACT1252.2379936E-710.6410544
ACCGGGG1701.4006218E-1010.61420215
TTATACT2700.010.5566024
TAATACT2001.8189894E-1210.45103554
GCGAACC1659.349606E-1010.36027311
GTAGGAC16050.010.35928253
GTATCAA35450.010.3060141
TCTATAC1856.002665E-1110.2712893
TATACTG3750.010.1343375
TGCGATC750.002655395310.13004512
CGTGTGA750.002655395310.13004510
ACTGTTC6100.010.1196668
GTTCTAG2650.010.0790691
TGTAGGA17850.010.0598212
TCCAACG2300.09.90982618
Produced by FastQC (version 0.11.5)