FastQCFastQC Report
Thu 26 May 2016
SRR1511693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62640.2520220302016056No Hit
GTCCTACAGTGGACATTTCTAAATT47630.19163169378196795No Hit
GTCCTAAAGTGTGTATTTCTCATTT43290.1741703973088682No Hit
CTGTAGGACGTGGAATATGGCAAGA42550.17119312555999866No Hit
GGTATCAACGCAGAGTACTTTTTTT37120.14934638826761812No Hit
CTTTAGGACGTGAAATATGGCGAGG36980.1487831206394536No Hit
TATCAACGCAGAGTACTTTTTTTTT36200.14564491528253704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG1101.5170372E-913.4803181
GCCGGTT1850.012.779211
GTCAACG707.5243865E-512.7100152
CCCCGTA905.7180296E-712.60880915
TCCAACG3150.012.60880918
TATACCG604.254707E-412.6088095
TAGGACC8100.012.3753144
CCAACGA2150.012.31558119
CGGTTTC2000.011.82075913
GGTATCA11850.011.7625731
GGCGAGG12050.011.69323319
GCCCCGT907.855688E-611.55807614
TAAGCCG600.006081018611.0327085
CCCGTAC1053.6638285E-610.80755116
CCGGTTT2100.010.80755112
GTATCAA25500.010.7772071
TTAGGAC17400.010.652273
GCGAACC803.935354E-410.63868311
AGGACCT16600.010.4820235
GGACCGT1002.5242893E-510.4022676
Produced by FastQC (version 0.11.5)