FastQCFastQC Report
Thu 26 May 2016
SRR1511693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485497
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79680.3205797472296285No Hit
GTCCTACAGTGGACATTTCTAAATT53180.21396123189848953No Hit
TATCAACGCAGAGTACTTTTTTTTT49210.19798857129982453No Hit
GGTATCAACGCAGAGTACTTTTTTT48990.19710343645556605No Hit
CTGTAGGACGTGGAATATGGCAAGA48890.19670110243544853No Hit
GTCCTAAAGTGTGTATTTCTCATTT46530.1872060195606754No Hit
CTTTAGGACGTGAAATATGGCGAGG44050.17722813586176125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG801.2248893E-714.3100191
TTACACG751.4777679E-512.6680494
TAGGACC8750.012.1613274
GGTATCA15700.012.0921181
GGCGAGG9900.012.08736219
GGACCGA855.3437296E-511.1727436
CCCGTTT1553.1468517E-1011.02857912
ATTAGAC1701.382432E-1010.6188053
AATCCCG3200.010.38757719
CTAGGAC3900.010.2318863
CGAAATC2053.6379788E-1210.19172213
GTCCTAA14050.010.1850671
GTCTACG750.002557755210.1760141
TGGCGAG25350.010.15243118
GTCCTAC27150.010.1197931
GGACCTG17350.09.9620946
CTAGTAC1252.567731E-69.8810783
GACGTGA13850.09.8739617
TTAGGAC16500.09.8465283
CCAACGA1651.027729E-89.78500719
Produced by FastQC (version 0.11.5)