FastQCFastQC Report
Thu 26 May 2016
SRR1511692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891874
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT59570.20599099407512222No Hit
TATCAACGCAGAGTACTTTTTTTTT34330.11871194941411693No Hit
GGTATCAACGCAGAGTACTTTTTTT34020.11763998016511093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13900.012.7994381
GTCCTAG2650.011.1894461
GTCCTAC8500.010.69798851
TAGGACC3300.010.6030514
GTAGGAC9200.010.4846863
GTATCAA29000.010.15667251
GTCTTAC2850.010.0574151
TCCTACA10100.09.9818732
GTCCTAA5650.09.6216041
GTCCTAT1853.3905962E-99.6168761
CTTACAC3450.09.593833
TGTAGGA10100.09.5904262
GCGATAA1002.885833E-49.45677511
GTCTAGG2750.09.3448831
CGATTAT850.0053706839.3025981
CGGTAGG850.0053706839.3025981
ACTATCG2451.8189894E-129.2637811
TAGGACG7250.09.2611174
TTACACT4800.09.2597594
AGGACGT6850.09.2496935
Produced by FastQC (version 0.11.5)