##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511692_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2891874 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.86603807773091 31.0 28.0 34.0 16.0 34.0 2 26.2574399852829 31.0 25.0 34.0 10.0 34.0 3 27.886830477399776 31.0 27.0 34.0 16.0 34.0 4 32.319409835974874 35.0 32.0 37.0 22.0 37.0 5 32.742041319919196 35.0 33.0 37.0 26.0 37.0 6 32.979222123785476 37.0 35.0 37.0 26.0 37.0 7 32.99544585967438 37.0 35.0 37.0 26.0 37.0 8 33.01210080383862 37.0 35.0 37.0 26.0 37.0 9 34.55096280128387 39.0 35.0 39.0 27.0 39.0 10 34.424946245929114 39.0 35.0 39.0 26.0 39.0 11 34.43159245527295 39.0 35.0 39.0 26.0 39.0 12 34.31808578105409 39.0 35.0 39.0 26.0 39.0 13 34.30500741041968 39.0 35.0 39.0 26.0 39.0 14 35.41098401935907 40.0 36.0 41.0 25.0 41.0 15 35.330102902131976 40.0 36.0 41.0 25.0 41.0 16 35.265182023836445 40.0 36.0 41.0 25.0 41.0 17 35.22081459980622 40.0 36.0 41.0 25.0 41.0 18 35.149662813801704 40.0 36.0 41.0 25.0 41.0 19 35.1636516667047 40.0 36.0 41.0 24.0 41.0 20 35.08106646416822 40.0 35.0 41.0 24.0 41.0 21 34.99087927067362 40.0 35.0 41.0 23.0 41.0 22 34.872224723483804 40.0 35.0 41.0 22.0 41.0 23 34.754115497424856 39.0 35.0 41.0 19.0 41.0 24 34.61829353561047 39.0 35.0 41.0 18.0 41.0 25 34.49449180704277 39.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1572755710487783 1101 2 3.6049596532521697 1101 3 2.1907724369552284 1101 4 1.293724689759479 1101 5 0.991424909664083 1101 6 0.7650164561848243 1101 7 0.6792063545423943 1101 8 0.4302012061680074 1101 9 0.41881944350729583 1101 10 0.6534852523768535 1101 11 0.46447092318510386 1101 12 0.5413428386988031 1101 13 0.6408633256597867 1101 14 0.7013776483101424 1101 15 0.5811334759772961 1101 16 0.6200842784832474 1101 17 0.7035170332927976 1101 18 0.6111441416556431 1101 19 0.7944543595441615 1101 20 0.7258566935166542 1101 21 0.8764009005652511 1101 22 0.6210335441641561 1101 23 0.7864056547530609 1101 24 0.7282355780689258 1101 25 0.6639791555127488 1102 1 1.5350385536117557 1102 2 4.1612968411338755 1102 3 2.7050520582640196 1102 4 1.8378053150766291 1102 5 1.3853249194699657 1102 6 1.073788216760022 1102 7 0.9988713740954509 1102 8 0.9859544837611836 1102 9 1.1065242286203798 1102 10 1.0583573807838036 1102 11 1.122922596199146 1102 12 1.1506481473001529 1102 13 1.153360909889635 1102 14 1.2489296837611192 1102 15 1.380663428195028 1102 16 1.2818409154646346 1102 17 1.2381019762139829 1102 18 1.2231581275764256 1102 19 1.1921629559412423 1102 20 1.3020593662390638 1102 21 1.2665868918592764 1102 22 0.9977443775088588 1102 23 1.0821190868158936 1102 24 0.9609236693772587 1102 25 0.8467353009305256 1103 1 1.6718345860362902 1103 2 4.131859014684885 1103 3 2.862408866923243 1103 4 1.8300461179174548 1103 5 1.3512611268076213 1103 6 1.1138308248717763 1103 7 1.1280145906805643 1103 8 0.9281239617099075 1103 9 1.065117808901448 1103 10 0.8546902669244432 1103 11 0.984879169460747 1103 12 1.1400529384121683 1103 13 1.073945015902872 1103 14 1.2162314011014743 1103 15 1.0160457007439732 1103 16 0.9632175916459218 1103 17 1.2441339051234692 1103 18 1.2941102990600228 1103 19 1.3245938030654187 1103 20 1.3018676019442452 1103 21 1.2958312556179266 1103 22 1.3619554164313001 1103 23 1.3094135072314756 1103 24 1.4803039835217575 1103 25 1.1886473329974692 1104 1 1.5933693974424088 1104 2 4.011444445194588 1104 3 2.611252087359773 1104 4 1.7779973829523428 1104 5 1.3546922126132444 1104 6 1.1606055068201115 1104 7 0.9558421412474303 1104 8 1.0062026717125505 1104 9 1.0085079182369654 1104 10 1.0196863741007576 1104 11 1.1749773488942452 1104 12 1.2278964675593542 1104 13 1.1080641841194847 1104 14 1.2297850090318505 1104 15 1.2387668641876743 1104 16 1.1706258505709357 1104 17 1.1722905011132951 1104 18 1.0953267548137546 1104 19 1.2964995099626506 1104 20 1.3519469045769057 1104 21 1.2939500684926344 1104 22 1.454358182903782 1104 23 1.1985357655060156 1104 24 1.1387066390236882 1104 25 1.1714485192019382 1105 1 1.6093662944907443 1105 2 4.01668834264726 1105 3 2.5502498975754335 1105 4 1.7275857761867286 1105 5 1.306268221299696 1105 6 1.0578577136411553 1105 7 0.9619197014097054 1105 8 1.009883735529769 1105 9 0.979563126243761 1105 10 0.8755825501557837 1105 11 0.8359134362675462 1105 12 1.0126945031138206 1105 13 0.9809970640493404 1105 14 0.9679155374422592 1105 15 1.0446546354687882 1105 16 1.0439517104701679 1105 17 1.045544806856256 1105 18 1.1916006005202817 1105 19 1.013521562272146 1105 20 1.0872056126041443 1105 21 1.1211456453099942 1105 22 1.0927121192910008 1105 23 1.095675530081479 1105 24 1.1334871608465704 1105 25 1.060218719053836 1106 1 1.0581199707809645 1106 2 3.4746779315482854 1106 3 2.0320630736061496 1106 4 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1107 17 1.3142088887024954 1107 18 1.323444101906503 1107 19 1.409519978829465 1107 20 1.4718844201282621 1107 21 1.454872861423361 1107 22 1.4878070636896723 1107 23 1.4219784513693483 1107 24 1.5261217506490183 1107 25 1.5776940886650763 1108 1 1.435775802590392 1108 2 3.9271851070172623 1108 3 2.5005491404803095 1108 4 1.5945093899413294 1108 5 1.1070813583804338 1108 6 1.032345934256135 1108 7 0.8762886478209069 1108 8 0.8919429073776755 1108 9 0.8199630331929626 1108 10 0.7511133626584297 1108 11 0.8204670039197097 1108 12 0.8876426907380619 1108 13 0.8827237907760619 1108 14 0.9822220975234686 1108 15 0.942877153761728 1108 16 0.8691092623882852 1108 17 0.9865950119909712 1108 18 0.9383692001057824 1108 19 0.9210903987142203 1108 20 0.9426321659180203 1108 21 0.9876685061990571 1108 22 1.1031729322166086 1108 23 1.1250171956907522 1108 24 1.0779791880850738 1108 25 1.2570475901784803 1109 1 1.294073666698118 1109 2 3.2990799375388526 1109 3 2.121543375118911 1109 4 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-2.1535513449034056 2315 19 -2.2719783398038444 2315 20 -2.290669273151174 2315 21 -2.3972404037825754 2315 22 -2.278038794666429 2315 23 -2.2891323756503397 2315 24 -2.2945713324293138 2315 25 -2.2375673822648707 2316 1 -0.7007102258798952 2316 2 1.5134075217193796 2316 3 0.22827377706990148 2316 4 -0.7020692799389181 2316 5 -0.9884315586942058 2316 6 -1.1013363734941102 2316 7 -1.6605626740138426 2316 8 -1.5946560984218294 2316 9 -1.9236763431721755 2316 10 -1.898205007455303 2316 11 -1.9049133530886522 2316 12 -2.1099998489922953 2316 13 -2.0167414498656484 2316 14 -2.156843579963919 2316 15 -2.210030129510095 2316 16 -2.380711856840456 2316 17 -2.280556829539499 2316 18 -2.439285767130791 2316 19 -2.411938523482057 2316 20 -2.1073151113283473 2316 21 -2.396058992482878 2316 22 -2.18187075235069 2316 23 -2.515014437961426 2316 24 -2.544763148286094 2316 25 -2.5117405396701287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 131282.0 3 4.0 4 1186.0 5 5661.0 6 6628.0 7 5908.0 8 5638.0 9 5203.0 10 5044.0 11 4882.0 12 4746.0 13 4791.0 14 4885.0 15 5047.0 16 5173.0 17 5771.0 18 6317.0 19 7955.0 20 10368.0 21 13250.0 22 16127.0 23 18914.0 24 21935.0 25 25053.0 26 27772.0 27 31262.0 28 36218.0 29 43104.0 30 53920.0 31 67578.0 32 85032.0 33 111413.0 34 149920.0 35 211002.0 36 330108.0 37 419894.0 38 1002883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1321094902475 17.75402386134389 12.815669009092373 25.298197639316232 2 17.34396922429702 20.398914581088007 38.30366430080618 23.95345189380879 3 19.092498497514068 23.623159238611365 28.982728846415856 28.301613417458714 4 12.970966231585471 16.15682425997813 36.933628505253 33.9385810031834 5 14.275034112827875 36.87954592765798 34.7149633766893 14.130456582824841 6 33.224856961264564 35.71891444786322 17.47783617128547 13.578392419586747 7 29.1090137398794 31.076734325216105 21.30663369150938 18.507618243395115 8 26.25684936480635 35.496290640601906 19.798476697117508 18.448383297474233 9 26.582001843787108 15.65735574924772 19.373561918672806 38.38708048829237 10 15.70901775111917 28.357355818407026 33.10507304260144 22.828553387872365 11 35.37349829211093 22.329430673673887 23.160137682347155 19.136933351868027 12 23.769880707112414 24.035936558785064 30.370929023878634 21.823253710223888 13 29.161609392387085 21.08262669812032 25.351761522113343 24.404002387379258 14 22.155114641924236 20.725245982362996 26.74898698905969 30.370652386653084 15 24.036558992542552 28.565732808552518 23.22182778364479 24.17588041526014 16 23.844296120785348 27.36485061244024 25.411688061098097 23.37916520567632 17 23.09882795723465 27.2716584470831 26.41954663308291 23.20996696259934 18 23.03976590957974 26.94266762659784 27.349013131277506 22.668553332544917 19 24.136225899270126 26.493971208279206 26.650824569405362 22.71897832304531 20 24.25997813182732 26.274381248975576 26.856668029105002 22.6089725900921 21 24.379276218192683 25.886054194765595 26.409280374678495 23.32538921236323 22 24.205429955319214 26.160097681516348 26.524708948986582 23.10976341417786 23 23.918099656414427 26.130450478862016 26.45857885039919 23.492871014324365 24 23.81020881622395 26.014454991626536 26.8001741431937 23.375162048955815 25 23.999143807786925 26.057497664144424 26.604029082871524 23.33932944519713 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 211.0 1 211.0 2 396.5 3 582.0 4 582.0 5 582.0 6 1491.0 7 2400.0 8 2400.0 9 2400.0 10 3545.0 11 4690.0 12 4690.0 13 4690.0 14 7578.5 15 10467.0 16 10467.0 17 10467.0 18 18732.0 19 26997.0 20 26997.0 21 26997.0 22 42271.5 23 57546.0 24 57546.0 25 57546.0 26 81024.0 27 104502.0 28 104502.0 29 104502.0 30 135053.5 31 165605.0 32 165605.0 33 165605.0 34 194050.0 35 222495.0 36 222495.0 37 222495.0 38 252800.0 39 283105.0 40 283105.0 41 283105.0 42 311630.0 43 340155.0 44 340155.0 45 340155.0 46 359233.0 47 378311.0 48 378311.0 49 378311.0 50 379973.5 51 381636.0 52 381636.0 53 381636.0 54 354741.5 55 327847.0 56 327847.0 57 327847.0 58 288957.0 59 250067.0 60 250067.0 61 250067.0 62 208558.5 63 167050.0 64 167050.0 65 167050.0 66 130951.5 67 94853.0 68 94853.0 69 94853.0 70 70093.5 71 45334.0 72 45334.0 73 45334.0 74 31903.5 75 18473.0 76 18473.0 77 18473.0 78 12569.0 79 6665.0 80 6665.0 81 6665.0 82 4363.5 83 2062.0 84 2062.0 85 2062.0 86 1334.5 87 607.0 88 607.0 89 607.0 90 378.0 91 149.0 92 149.0 93 149.0 94 98.0 95 47.0 96 47.0 97 47.0 98 32.5 99 18.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.323009923668873 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.4579653193742187E-5 20 0.0 21 2.766372255499375E-4 22 9.33650636231039E-4 23 6.224337574873594E-4 24 3.4579653193742187E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2891874.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.80827460229418 #Duplication Level Percentage of deduplicated Percentage of total 1 77.83768320285937 52.78038997025869 2 14.025465162244508 19.020851862927724 3 4.142794493533273 8.427472399151291 4 1.6959244406634475 4.599908407089968 5 0.8349252171601407 2.8307419198787462 6 0.45253392154416133 1.8411326651351727 7 0.2627725325715178 1.2472706432584841 8 0.18254542655856482 0.990247232918087 9 0.11477866346897724 0.7004648817889849 >10 0.4273581014202855 4.621091496783391 >50 0.01334640241649364 0.6164159210748011 >100 0.008586459304314899 1.126270600771472 >500 6.17268602523563E-4 0.3050598889076151 >1k 6.172686022784348E-4 0.6849023527155503 >5k 5.143905018986957E-5 0.20777975733991308 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5957 0.20599099407512222 No Hit TATCAACGCAGAGTACTTTTTTTTT 3433 0.11871194941411693 No Hit GGTATCAACGCAGAGTACTTTTTTT 3402 0.11763998016511093 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.4579653193742187E-5 0.0 0.0 0.0 0.0 7 3.4579653193742187E-5 0.0 0.0 0.0 0.0 8 3.4579653193742187E-5 3.4579653193742187E-5 0.0 3.4579653193742187E-5 0.0 9 3.4579653193742187E-5 3.4579653193742187E-5 0.0 3.4579653193742187E-5 3.4579653193742187E-5 10 6.915930638748437E-5 3.4579653193742187E-5 0.0 3.4579653193742187E-5 3.4579653193742187E-5 11 6.915930638748437E-5 3.4579653193742187E-5 0.0 3.4579653193742187E-5 3.4579653193742187E-5 12 6.915930638748437E-5 3.4579653193742187E-5 0.0 3.4579653193742187E-5 3.4579653193742187E-5 13 6.915930638748437E-5 3.4579653193742187E-5 0.0 3.4579653193742187E-5 3.4579653193742187E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1390 0.0 12.799438 1 GTCCTAG 265 0.0 11.189446 1 GTCCTAC 850 0.0 10.6979885 1 TAGGACC 330 0.0 10.603051 4 GTAGGAC 920 0.0 10.484686 3 GTATCAA 2900 0.0 10.1566725 1 GTCTTAC 285 0.0 10.057415 1 TCCTACA 1010 0.0 9.981873 2 GTCCTAA 565 0.0 9.621604 1 GTCCTAT 185 3.3905962E-9 9.616876 1 CTTACAC 345 0.0 9.59383 3 TGTAGGA 1010 0.0 9.590426 2 GCGATAA 100 2.885833E-4 9.456775 11 GTCTAGG 275 0.0 9.344883 1 CGATTAT 85 0.005370683 9.302598 1 CGGTAGG 85 0.005370683 9.302598 1 ACTATCG 245 1.8189894E-12 9.26378 11 TAGGACG 725 0.0 9.261117 4 TTACACT 480 0.0 9.259759 4 AGGACGT 685 0.0 9.249693 5 >>END_MODULE