FastQCFastQC Report
Thu 26 May 2016
SRR1511692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891874
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69680.24095102345399558No Hit
TATCAACGCAGAGTACTTTTTTTTT43320.14979905763529117No Hit
GGTATCAACGCAGAGTACTTTTTTT42470.14685978711382308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.012.4052231
GTCCTAC8550.011.2698751
CTTACAC3700.010.5276593
ATTAGAC2300.010.3267073
GTATCAA33650.09.9797911
TAGGACC4550.09.8137764
GTATAAG3550.09.674711
GTCCTAA5350.09.6295011
GAGTAGG2550.09.3532741
TCCTACA10850.09.2816642
AGGACCG2054.2382453E-109.2688495
GTAGGAC9600.09.1047143
ACTATCG2401.0913936E-119.10125111
TCTTACA4550.08.9785612
CTACACT5200.08.9524614
TAGAACA6550.08.847864
TGGCGAG7500.08.73720118
GTGTAAG3500.08.7225961
TTACACC2850.08.6671884
TCAGGAC6650.08.5719433
Produced by FastQC (version 0.11.5)