FastQCFastQC Report
Thu 26 May 2016
SRR1511691_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511691_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621574
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37220.22953007386650257No Hit
GTCCTACAGTGGACATTTCTAAATT27420.16909496575549438No Hit
GGTATCAACGCAGAGTACTTTTTTT23490.14485925403342678No Hit
CTGTAGGACGTGGAATATGGCAAGA23300.1436875529578052No Hit
TATCAACGCAGAGTACTTTTTTTTT20980.12938046613968898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2150.014.51527718
ACCGTCC500.001552193913.2392838
CCAACGA1207.9489837E-1012.6092319
CCAACGT1302.1282176E-1012.36674619
TGGACCG701.1385529E-412.1585255
TTAGACT1405.638867E-1112.1585254
TAGGACC6300.012.1585254
GCACCGT550.00317549812.0357126
GTCCTAC11900.011.7119481
CCGGTTT1950.011.6389312
GGTATCA8600.011.6086031
GTAGGAC13850.011.6074173
TGGCGAG11450.011.56304918
GCCGGTT2050.011.53247711
AGGACGT10750.011.5238955
GGCGAGG4700.011.46903319
TGTAGGA13650.011.441462
GTGCGCC752.1639332E-411.34795811
GACTGTC2000.011.3479577
GGACGTG11150.011.1952946
Produced by FastQC (version 0.11.5)