##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511691_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1621574 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.385461903064552 31.0 30.0 34.0 25.0 34.0 2 26.701844627503895 31.0 26.0 34.0 10.0 34.0 3 28.396483910077492 31.0 28.0 34.0 16.0 34.0 4 32.87587060473343 35.0 33.0 37.0 26.0 37.0 5 33.325213650440865 35.0 35.0 37.0 28.0 37.0 6 33.582062243227874 37.0 35.0 37.0 28.0 37.0 7 33.62801142593554 37.0 35.0 37.0 28.0 37.0 8 33.64843602573796 37.0 35.0 37.0 28.0 37.0 9 35.24514144898722 39.0 35.0 39.0 29.0 39.0 10 35.117639404677185 39.0 35.0 39.0 28.0 39.0 11 35.138006036110596 39.0 35.0 39.0 29.0 39.0 12 35.02911060488143 39.0 35.0 39.0 28.0 39.0 13 35.03281194691084 39.0 35.0 39.0 28.0 39.0 14 36.1736201986465 40.0 36.0 41.0 28.0 41.0 15 36.1169234336515 40.0 36.0 41.0 27.0 41.0 16 36.05832666285966 40.0 36.0 41.0 27.0 41.0 17 36.02434918172097 40.0 36.0 41.0 27.0 41.0 18 35.9623051430277 40.0 36.0 41.0 27.0 41.0 19 35.98895270891122 40.0 36.0 41.0 27.0 41.0 20 35.91073981205915 40.0 36.0 41.0 27.0 41.0 21 35.828024499652805 40.0 36.0 41.0 27.0 41.0 22 35.7220688047539 40.0 36.0 41.0 27.0 41.0 23 35.61239511733661 40.0 36.0 41.0 27.0 41.0 24 35.49552965205412 40.0 36.0 41.0 27.0 41.0 25 35.38805691260467 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0111474624410661 1101 2 3.466813122785254 1101 3 2.00969167244185 1101 4 1.0878416697215911 1101 5 0.6393488736640549 1101 6 0.4789323402537704 1101 7 0.4433875488622405 1101 8 0.023390348828193908 1101 9 0.16270574647859348 1101 10 0.4983965974959119 1101 11 0.2109181469998873 1101 12 0.21202319538572567 1101 13 0.25910924092499954 1101 14 0.1708006016207264 1101 15 0.2270390330512484 1101 16 0.35704926609697196 1101 17 0.40423720031400734 1101 18 0.3638410268114143 1101 19 0.4530985679815629 1101 20 0.4784693868834822 1101 21 0.6044865181545589 1101 22 0.38340434511602695 1101 23 0.47112541074159253 1101 24 0.598729172692245 1101 25 0.3844313274904181 1102 1 1.1213176712777688 1102 2 3.8886340187670356 1102 3 2.540636285316779 1102 4 1.5289611083094456 1102 5 0.9493855778300215 1102 6 0.6645076799789109 1102 7 0.6172340992862217 1102 8 0.6860582877007531 1102 9 0.721282363539494 1102 10 0.7279111329612178 1102 11 0.7555691111575413 1102 12 0.8133520862581634 1102 13 0.7502102120079641 1102 14 0.8003501483973778 1102 15 0.803975427886499 1102 16 0.8628799960251001 1102 17 0.9031099130365732 1102 18 0.9381246415177031 1102 19 0.7756354645173644 1102 20 0.7007142659982719 1102 21 0.8283299906610608 1102 22 0.5353861392341273 1102 23 0.6931586830295657 1102 24 0.5561038629767978 1102 25 0.4942533469120747 1103 1 1.229515973031635 1103 2 3.822574839709599 1103 3 2.3198494559942233 1103 4 1.3803478857205462 1103 5 0.9331067020837907 1103 6 0.7381329534410312 1103 7 0.6543164901794682 1103 8 0.5146281271460396 1103 9 0.6262528852496061 1103 10 0.3531058009133048 1103 11 0.46557967884636753 1103 12 0.5081702107714889 1103 13 0.6118031520502356 1103 14 0.7641864048399611 1103 15 0.5285944348945435 1103 16 0.5865747937558297 1103 17 0.7630657883007714 1103 18 0.6927215861578446 1103 19 0.7082330765904885 1103 20 0.7487026971599775 1103 21 0.7431201200689017 1103 22 1.0534133185881984 1103 23 0.8129165531767697 1103 24 0.972053657184965 1103 25 0.7462435949746649 1104 1 1.39695270561419 1104 2 3.9800224876477373 1104 3 2.524373848964558 1104 4 1.5450259704044171 1104 5 1.12430690899334 1104 6 1.0202856895129955 1104 7 0.7732009320716386 1104 8 0.8503336141944686 1104 9 0.8948960057261957 1104 10 0.7657610378729629 1104 11 0.9722498816790974 1104 12 0.7737912243652758 1104 13 0.6648815891817321 1104 14 0.944196837991079 1104 15 0.8727700625274935 1104 16 0.635956037755669 1104 17 0.7337021666822281 1104 18 0.6309025355745987 1104 19 0.8611747430014418 1104 20 0.9049338747471154 1104 21 1.0215291960336401 1104 22 1.13501634157182 1104 23 0.9571855531285465 1104 24 0.7968819266408218 1104 25 0.9546532408174073 1105 1 1.4526368432244183 1105 2 3.852529419933198 1105 3 2.5039775994014413 1105 4 1.5105552782105107 1105 5 1.17595427573427 1105 6 0.9157981781414364 1105 7 0.8764948243843236 1105 8 0.7996594801886516 1105 9 0.8861343876027021 1105 10 0.7426246797233205 1105 11 0.85073290036609 1105 12 0.7606782381011428 1105 13 0.8097296593083243 1105 14 0.8662162496521262 1105 15 0.8781581716759206 1105 16 0.6982071831126433 1105 17 0.9587027239568044 1105 18 1.0294791932141152 1105 19 1.1123554290842534 1105 20 1.098175248662372 1105 21 1.1767480409148945 1105 22 1.2701905456049687 1105 23 1.207387959461208 1105 24 1.0547600016924576 1105 25 1.2792911280023205 1106 1 1.1232011469181487 1106 2 3.8372090580567395 1106 3 2.193088449085998 1106 4 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1.1965290006619185 1107 18 1.0772862382101422 1107 19 1.0996923389610203 1107 20 1.2051393051725583 1107 21 1.2960240781024837 1107 22 1.4821946303068003 1107 23 1.4490035602098956 1107 24 1.328777131031913 1107 25 1.266670050975165 1108 1 1.420439889483994 1108 2 3.937095318916775 1108 3 2.474983370564882 1108 4 1.504883209478102 1108 5 1.1914102417772838 1108 6 0.8776090035361648 1108 7 0.7867591504901625 1108 8 0.7099322313506065 1108 9 0.7621035198018618 1108 10 0.6567019455117702 1108 11 0.7628198469452201 1108 12 0.813185996173651 1108 13 0.9396544556526507 1108 14 0.907162423573368 1108 15 0.8658375300878376 1108 16 0.9039261914434249 1108 17 0.8532579311429771 1108 18 0.8632227465417088 1108 19 0.7047937406227689 1108 20 0.8173576972645193 1108 21 0.8040287738145366 1108 22 0.9135065674539504 1108 23 0.9128287403435493 1108 24 0.9973312084613895 1108 25 0.9865998641837237 1109 1 1.3732556814657855 1109 2 3.3726833414991724 1109 3 2.2411254692479474 1109 4 1.657735629648272 1109 5 1.1748696246670605 1109 6 1.0219599464316929 1109 7 0.9578139115005797 1109 8 0.7217743489084043 1109 9 0.9307109198171872 1109 10 0.9414008935726272 1109 11 0.88779335691612 1109 12 1.0110282968723894 1109 13 0.9256950045522174 1109 14 0.8283075403444045 1109 15 0.8194355955214832 1109 16 0.8129735408520489 1109 17 0.9114825479018691 1109 18 0.80930082064652 1109 19 0.8263099981189299 1109 20 0.866054126203025 1109 21 0.9637624345590226 1109 22 1.0173818671603598 1109 23 1.0879253098147004 1109 24 1.119942200281713 1109 25 1.038257943782753 1110 1 1.2675486027410159 1110 2 3.724262908489756 1110 3 2.2747054043147976 1110 4 1.490726993904694 1110 5 0.9751127376369695 1110 6 0.7873808669314357 1110 7 0.6613084584928188 1110 8 0.6933622327367885 1110 9 0.5939485299064131 1110 10 0.7198874679205218 1110 11 0.7205810148412084 1110 12 0.7077780569906267 1110 13 0.6876350053108453 1110 14 0.6104896285169303 1110 15 0.8411032760368968 1110 16 0.9273297094309783 1110 17 0.8628638173154357 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-1.8382702896004446 2315 15 -1.6766091583760385 2315 16 -1.994071551677436 2315 17 -1.7080529877154689 2315 18 -1.8093417047183777 2315 19 -1.8315256174204961 2315 20 -2.0293172639152317 2315 21 -1.9805270357202929 2315 22 -1.8140948715476242 2315 23 -1.9930516836410916 2315 24 -1.9115979313204505 2315 25 -1.7845062959389608 2316 1 -0.5792857207170776 2316 2 1.8703086472553032 2316 3 0.3172043711146344 2316 4 -0.47877470493239116 2316 5 -0.5577890492202329 2316 6 -0.8365348824526038 2316 7 -1.3121236678757953 2316 8 -1.3200748554308461 2316 9 -1.5813864524834713 2316 10 -1.5243576985923326 2316 11 -1.4083565749923466 2316 12 -1.5138464262569045 2316 13 -1.3739208661310087 2316 14 -1.8710154789663633 2316 15 -1.6328043052750374 2316 16 -1.7520603550236444 2316 17 -1.7158248901420166 2316 18 -1.9439892293080945 2316 19 -1.816109669951615 2316 20 -1.7327429285453135 2316 21 -1.972001687993874 2316 22 -1.9284222047165684 2316 23 -2.0777845986981305 2316 24 -1.776375048501741 2316 25 -1.8114385406579956 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54795.0 3 2.0 4 461.0 5 2472.0 6 2972.0 7 2660.0 8 2383.0 9 2420.0 10 2255.0 11 2261.0 12 2204.0 13 2236.0 14 2223.0 15 2319.0 16 2342.0 17 2656.0 18 2977.0 19 3708.0 20 4873.0 21 6002.0 22 7442.0 23 8987.0 24 10479.0 25 11739.0 26 13665.0 27 15723.0 28 18291.0 29 22075.0 30 27640.0 31 35413.0 32 46539.0 33 61739.0 34 85156.0 35 121292.0 36 192033.0 37 248191.0 38 590949.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.45322877648507 17.883056832435646 12.170459072481428 25.493255318597857 2 16.769163436852814 20.002114177169336 40.03349166028009 23.195230725697762 3 20.281405597277708 22.340454398010824 28.560707066097507 28.817432938613962 4 12.29040426153848 15.444808562544788 37.48610917540612 34.77867800051062 5 13.47018390773409 37.18270026529779 35.703335154609036 13.64378067235908 6 33.68911933713787 36.257673100333385 17.087965149909902 12.96524241261885 7 29.650697408813905 30.57997969873715 20.456297399933646 19.313025492515294 8 25.336185705986896 38.169704250314815 19.145102227835427 17.349007815862862 9 25.855310950964927 15.74747745092114 19.323077454374577 39.07413414373935 10 14.61246911950981 29.447869785776042 34.067825458474296 21.87183563623985 11 36.25662473621309 22.112527704563593 22.88171862647033 18.749128932752992 12 23.171868813880835 24.081355522473842 31.687113878244222 21.059661785401097 13 29.769162554407014 21.08025905694097 25.276552288085526 23.874026100566486 14 20.701429598649213 21.551097883907858 27.182724932688856 30.56474758475407 15 23.313027959254402 30.512391047217086 22.835405599744448 23.339175393784064 16 22.525089820137715 28.286467345924393 27.07702516197226 22.111417671965633 17 21.920307059683985 28.20401659128723 27.456902984384307 22.418773364644476 18 21.70841417042947 27.75722847060942 29.074158811130417 21.46019854783069 19 23.39586315263019 26.805638722401028 27.102572625469218 22.695925499499563 20 23.123411650292645 27.327292696659356 28.090317241345286 21.458978411702713 21 23.00434393292435 26.70593720167837 27.070645028404538 23.219073836992745 22 22.73330788457281 27.66066482934016 27.601215866952728 22.004811419134302 23 22.75582262298782 27.23896635530041 27.39030182607768 22.614909195634088 24 22.894004771909746 26.651652438712286 28.329467745207893 22.124875044170075 25 22.858037931047242 27.048164314425367 27.898264279027664 22.195533475499733 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 104.0 1 104.0 2 210.0 3 316.0 4 316.0 5 316.0 6 845.5 7 1375.0 8 1375.0 9 1375.0 10 2245.5 11 3116.0 12 3116.0 13 3116.0 14 4883.0 15 6650.0 16 6650.0 17 6650.0 18 11458.5 19 16267.0 20 16267.0 21 16267.0 22 25583.5 23 34900.0 24 34900.0 25 34900.0 26 50357.5 27 65815.0 28 65815.0 29 65815.0 30 87132.5 31 108450.0 32 108450.0 33 108450.0 34 124579.5 35 140709.0 36 140709.0 37 140709.0 38 155453.0 39 170197.0 40 170197.0 41 170197.0 42 184263.5 43 198330.0 44 198330.0 45 198330.0 46 206684.5 47 215039.0 48 215039.0 49 215039.0 50 211079.0 51 207119.0 52 207119.0 53 207119.0 54 188640.0 55 170161.0 56 170161.0 57 170161.0 58 147761.5 59 125362.0 60 125362.0 61 125362.0 62 103324.5 63 81287.0 64 81287.0 65 81287.0 66 62835.0 67 44383.0 68 44383.0 69 44383.0 70 32298.0 71 20213.0 72 20213.0 73 20213.0 74 14064.0 75 7915.0 76 7915.0 77 7915.0 78 5338.0 79 2761.0 80 2761.0 81 2761.0 82 1780.5 83 800.0 84 800.0 85 800.0 86 509.0 87 218.0 88 218.0 89 218.0 90 137.5 91 57.0 92 57.0 93 57.0 94 40.0 95 23.0 96 23.0 97 23.0 98 15.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.32561202880658 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 6.166847766429408E-5 20 6.166847766429408E-5 21 1.2333695532858817E-4 22 9.250271649644112E-4 23 6.783532543072348E-4 24 6.166847766429408E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1621574.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.92673380123153 #Duplication Level Percentage of deduplicated Percentage of total 1 86.62359257761865 67.50293639702424 2 9.691988871393105 15.10530073571098 3 2.075997211342052 4.853270461810533 4 0.6736269186693205 2.0997418228995195 5 0.3187472902557314 1.2419467618811129 6 0.1717700058366528 0.8031285311921299 7 0.0947491880396845 0.5168446327975927 8 0.06770024736286344 0.4220527323618709 9 0.047425652516490266 0.3326153579102019 >10 0.21037371434321755 2.847529233035144 >50 0.012046997791369758 0.6533801901496215 >100 0.009745081080812432 1.4967361362706009 >500 0.0011181218749724503 0.5738859655831036 >1k 0.0011181218749724503 1.5506310413732813 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3722 0.22953007386650257 No Hit GTCCTACAGTGGACATTTCTAAATT 2742 0.16909496575549438 No Hit GGTATCAACGCAGAGTACTTTTTTT 2349 0.14485925403342678 No Hit CTGTAGGACGTGGAATATGGCAAGA 2330 0.1436875529578052 No Hit TATCAACGCAGAGTACTTTTTTTTT 2098 0.12938046613968898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.166847766429407E-5 0.0 3 0.0 0.0 0.0 6.166847766429407E-5 0.0 4 0.0 0.0 0.0 6.166847766429407E-5 0.0 5 0.0 6.166847766429407E-5 0.0 6.166847766429407E-5 0.0 6 0.0 6.166847766429407E-5 0.0 6.166847766429407E-5 6.166847766429407E-5 7 0.0 6.166847766429407E-5 0.0 1.850054329928822E-4 6.166847766429407E-5 8 0.0 6.166847766429407E-5 0.0 1.850054329928822E-4 6.166847766429407E-5 9 0.0 6.166847766429407E-5 0.0 1.850054329928822E-4 6.166847766429407E-5 10 0.0 6.166847766429407E-5 0.0 3.083423883214704E-4 6.166847766429407E-5 11 0.0 6.166847766429407E-5 0.0 3.700108659857644E-4 6.166847766429407E-5 12 0.0 6.166847766429407E-5 0.0 3.700108659857644E-4 6.166847766429407E-5 13 0.0 6.166847766429407E-5 0.0 3.700108659857644E-4 6.166847766429407E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 215 0.0 14.515277 18 ACCGTCC 50 0.0015521939 13.239283 8 CCAACGA 120 7.9489837E-10 12.60923 19 CCAACGT 130 2.1282176E-10 12.366746 19 TGGACCG 70 1.1385529E-4 12.158525 5 TTAGACT 140 5.638867E-11 12.158525 4 TAGGACC 630 0.0 12.158525 4 GCACCGT 55 0.003175498 12.035712 6 GTCCTAC 1190 0.0 11.711948 1 CCGGTTT 195 0.0 11.63893 12 GGTATCA 860 0.0 11.608603 1 GTAGGAC 1385 0.0 11.607417 3 TGGCGAG 1145 0.0 11.563049 18 GCCGGTT 205 0.0 11.532477 11 AGGACGT 1075 0.0 11.523895 5 GGCGAGG 470 0.0 11.469033 19 TGTAGGA 1365 0.0 11.44146 2 GTGCGCC 75 2.1639332E-4 11.347958 11 GACTGTC 200 0.0 11.347957 7 GGACGTG 1115 0.0 11.195294 6 >>END_MODULE