FastQCFastQC Report
Thu 26 May 2016
SRR1511691_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511691_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621574
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48310.2979204155962047No Hit
GGTATCAACGCAGAGTACTTTTTTT31720.19561241115114084No Hit
TATCAACGCAGAGTACTTTTTTTTT29930.18457375364923215No Hit
GTCCTACAGTGGACATTTCTAAATT29090.17939360152543146No Hit
CTGTAGGACGTGGAATATGGCAAGA24210.14929938442525595No Hit
GTCCTACAGTGTGCATTTCTCATTT18020.11112659675105792No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17190.10600811310492152No Hit
GTCCTAAAGTGTGTATTTCTCATTT16730.103171363132364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCCG400.00528196314.248895
ACTAGAC751.479861E-512.665683
GCACCGT604.1012812E-412.6633386
ACCGTGC1207.4396667E-1012.6633388
TAGGACC5650.012.273384
ACTGTTC6750.011.8191148
GTATTAA1950.011.7384591
GATATAC6400.011.4748021
GTAGGAC13100.011.4571233
CGGGACT752.0754214E-411.3991134
TCCAACG2000.011.39700218
CTTACTC1104.973099E-711.22639753
TGTAGGA14350.011.1210842
GGACCGT600.00588735411.08042056
GACCGTG951.3641535E-510.9971087
AGGACGT9550.010.9415565
GGTATCA9450.010.8999991
GTACTAG700.001443235410.8999981
TGGACCG1053.4691457E-610.8562975
CCGTGCC700.00149473810.8542889
Produced by FastQC (version 0.11.5)