FastQCFastQC Report
Thu 26 May 2016
SRR1511690_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511690_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697962
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT51200.30153796139136213No Hit
TATCAACGCAGAGTACTTTTTTTTT30270.178272540846026No Hit
GGTATCAACGCAGAGTACTTTTTTT30120.17738912884976224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG2550.012.2382319
GGTATCA9750.011.2544031
TCCAACG1950.011.15419118
ACTGTTC5950.010.8074698
GTATTGG1751.1459633E-1010.7329641
ATAATAC1605.8935257E-1010.6386033
GGACGTG7050.010.5966856
AGGACGT7350.010.2928285
CCCGTTT1201.6158301E-610.2445812
GATATAC6000.010.2151451
TAGGACT2600.09.8202494
CCAACGA1356.8553345E-79.80706619
GTATCAA18900.09.7810141
CTAATAC1554.3602995E-89.7615853
GTCTTAG2950.09.7180711
TAGGACG7400.09.7121184
GACGTGG3800.09.7053927
TAGACAG2151.0913936E-119.67645555
GGTCTAC2251.0913936E-119.6659431
GTCTAGG1655.7700163E-89.5860611
Produced by FastQC (version 0.11.5)