FastQCFastQC Report
Thu 26 May 2016
SRR1511690_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511690_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697962
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64470.37969047599416245No Hit
GGTATCAACGCAGAGTACTTTTTTT40480.23840345072504565No Hit
TATCAACGCAGAGTACTTTTTTTTT40410.23799119179345593No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21210.12491445627169513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA851.7234925E-814.52515619
GGACCGT701.0940025E-412.2107096
GGTATCA11700.011.819131
TCCAACG1459.822543E-1111.78964818
TGGACCG752.072866E-411.4006895
TGTAGGA9400.010.3091342
TTAGGAC5400.010.2043213
TAGACAG2900.010.15578655
TAGGACG8050.010.1496834
AACCGGG856.611394E-410.05587814
GGCGAGG3700.010.0105819
AGGACGT7900.09.9816165
TGGCGAG7900.09.97808818
GTCTAAG2201.8189894E-129.9703071
TAACCTG1252.567538E-69.8805975
GATATAC5800.09.865671
ATAGCCC1752.568413E-99.7720193
ACTGTTC6250.09.7251518
TAGGACA3850.09.6239584
GGACGTG7600.09.6221816
Produced by FastQC (version 0.11.5)