##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511690_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697962 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.647687050711383 31.0 31.0 34.0 27.0 34.0 2 30.83330133418769 31.0 31.0 34.0 27.0 34.0 3 30.9399114938968 31.0 31.0 34.0 27.0 34.0 4 34.529535407741754 37.0 35.0 37.0 31.0 37.0 5 34.13355775924314 37.0 35.0 37.0 30.0 37.0 6 34.0731800829465 37.0 35.0 37.0 30.0 37.0 7 33.87982122096961 37.0 35.0 37.0 29.0 37.0 8 33.78167355924337 37.0 35.0 37.0 29.0 37.0 9 35.23323490160557 39.0 35.0 39.0 29.0 39.0 10 35.02522435719998 38.0 35.0 39.0 28.0 39.0 11 35.02799768192692 38.0 35.0 39.0 28.0 39.0 12 34.82687009485489 38.0 35.0 39.0 27.0 39.0 13 34.809701866119504 38.0 35.0 39.0 27.0 39.0 14 35.76318080145492 39.0 36.0 41.0 27.0 41.0 15 35.66784945717278 39.0 36.0 41.0 27.0 41.0 16 35.49550225505636 39.0 35.0 41.0 27.0 41.0 17 35.45173213534814 39.0 35.0 40.0 27.0 41.0 18 35.373658538883674 39.0 35.0 40.0 27.0 41.0 19 35.38959411341361 39.0 35.0 40.0 27.0 41.0 20 35.498810338511696 39.0 36.0 41.0 27.0 41.0 21 35.437343120753 39.0 36.0 41.0 27.0 41.0 22 35.319925298681596 39.0 35.0 41.0 27.0 41.0 23 35.20659355156358 39.0 35.0 41.0 26.0 41.0 24 35.020031661485945 39.0 35.0 41.0 25.0 41.0 25 34.45527579533582 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2885235030443454 1101 2 0.30014949852444417 1101 3 0.3740731874714598 1101 4 0.4398011869157088 1101 5 0.40249714579523754 1101 6 0.31058839833672636 1101 7 0.4683325753904626 1101 8 0.19341340636310633 1101 9 0.3358631530006164 1101 10 0.5698229685397749 1101 11 0.36030922082658634 1101 12 0.5925691946400704 1101 13 0.4281725019164 1101 14 0.40270355315591644 1101 15 0.20837871907942684 1101 16 0.28056005741992607 1101 17 0.19987425896535882 1101 18 0.38471024876472626 1101 19 0.3856495460910665 1101 20 0.018036487885716213 1101 21 0.3581266753296717 1101 22 0.3026796920005381 1101 23 0.2633020030085973 1101 24 -0.11527577777513187 1101 25 0.32625340509947165 1102 1 0.5268013618517848 1102 2 0.5848052483989541 1102 3 0.49494301010161124 1102 4 0.6116868973105767 1102 5 0.7848900299100876 1102 6 0.6499215249094874 1102 7 0.6717455364551199 1102 8 0.624818510526012 1102 9 0.5922259189920709 1102 10 0.7118454772098133 1102 11 0.8315136592868768 1102 12 0.6820628928020156 1102 13 0.8388508966406008 1102 14 0.9293397537767802 1102 15 0.8872193486113957 1102 16 0.7994419062081946 1102 17 0.9540990459043996 1102 18 0.8165799414429529 1102 19 0.9990018781468848 1102 20 1.08503770810524 1102 21 0.8690386928935254 1102 22 0.9479379205938656 1102 23 1.007663314202773 1102 24 1.0970631783663052 1102 25 1.2346152925660832 1103 1 0.5565789516671131 1103 2 0.6123586158787973 1103 3 0.6364088521432691 1103 4 0.7173844488586809 1103 5 0.7242275586617168 1103 6 0.5631033556878506 1103 7 0.6306504382989573 1103 8 0.6355432142140529 1103 9 0.7013296875187223 1103 10 0.9367413448071318 1103 11 0.6774242108482298 1103 12 0.8208888263187362 1103 13 0.9932668695932847 1103 14 1.1799358718702138 1103 15 0.9732434969667807 1103 16 1.1651300837314977 1103 17 1.2317858546755005 1103 18 1.213410846325516 1103 19 1.1058693754630013 1103 20 1.0447896458655705 1103 21 1.2464130743344057 1103 22 1.3889665089597116 1103 23 1.2440333255166607 1103 24 1.2957286449650098 1103 25 1.0893082691563265 1104 1 0.3574351387712298 1104 2 0.38093397299200404 1104 3 0.46104374661747016 1104 4 0.46563603160985423 1104 5 0.41509311152471184 1104 6 0.5882904186805362 1104 7 0.5995257790395456 1104 8 0.5491004411734721 1104 9 0.6368103170032526 1104 10 0.8129837433725484 1104 11 0.8585934486368529 1104 12 0.9016392044020805 1104 13 0.7335594971813819 1104 14 1.0086759483947532 1104 15 1.0845206621292647 1104 16 0.8964705356623455 1104 17 0.9319426748800836 1104 18 0.8158246825077597 1104 19 0.8765914079645896 1104 20 0.9800452488318854 1104 21 1.1374494145651894 1104 22 1.071459135461474 1104 23 0.8411935684521907 1104 24 1.0600025037895833 1104 25 1.2154336927299525 1105 1 0.3471716100992879 1105 2 0.3303483744177953 1105 3 0.33709869207361365 1105 4 0.3910743830077408 1105 5 0.4185048500211792 1105 6 0.32227577480448844 1105 7 0.42274832586991806 1105 8 0.31105735894080766 1105 9 0.3715914225734238 1105 10 0.2904043528361555 1105 11 0.46256467052204897 1105 12 0.5252690885192095 1105 13 0.6209792050936471 1105 14 0.5030822321330746 1105 15 0.4226724520812155 1105 16 0.49222795427498056 1105 17 0.5834759454773675 1105 18 0.5861310087903675 1105 19 0.6209233695987351 1105 20 0.5085648195742323 1105 21 0.5797702461382599 1105 22 0.5359953841585039 1105 23 0.5451676961640146 1105 24 0.5721186832087284 1105 25 0.7065157853125683 1106 1 0.14234720926650013 1106 2 0.24270842081527277 1106 3 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1107 15 0.8901291829267493 1107 16 1.043938511140297 1107 17 1.0764680201045493 1107 18 0.8333305885843032 1107 19 0.9668890923889748 1107 20 0.9981207572919004 1107 21 0.860078033054215 1107 22 0.9489401228530099 1107 23 0.9053489635518659 1107 24 0.9481182136882538 1107 25 0.9898064299324432 1108 1 0.15982171224843 1108 2 0.16685090546794612 1108 3 0.17644801972456392 1108 4 0.19703339235895356 1108 5 0.33452435029769845 1108 6 0.20964237937260322 1108 7 0.0562624910296563 1108 8 0.1516700226324943 1108 9 0.2260195257235651 1108 10 0.24856716032118698 1108 11 0.3248820568209325 1108 12 0.1399295306202717 1108 13 0.16253042203965862 1108 14 0.2841632933353466 1108 15 0.2260487500477879 1108 16 0.41748413269571927 1108 17 0.47523217871154344 1108 18 0.3554518719777633 1108 19 0.32570937126099864 1108 20 0.3726961280674459 1108 21 0.5347149966390248 1108 22 0.6591571636725675 1108 23 0.5847486062352942 1108 24 0.4908053040702711 1108 25 0.6183281349039547 1109 1 -0.15174591192980103 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-1.2358256074121243 2315 9 -1.3195372779850985 2315 10 -1.4460115098476578 2315 11 -1.2305981152298102 2315 12 -1.199030511679993 2315 13 -1.2892001704736913 2315 14 -1.4372086517163893 2315 15 -1.6155657301582238 2315 16 -1.5369907767714324 2315 17 -1.5971790717404772 2315 18 -1.6997831617328885 2315 19 -1.6316452749296744 2315 20 -1.7018671093578277 2315 21 -1.791622901087031 2315 22 -1.7058530732203465 2315 23 -1.7619719958761735 2315 24 -1.6440057530365877 2315 25 -1.6906375556481947 2316 1 -1.0908585928174013 2316 2 -1.1489048049485326 2316 3 -1.2143394392741271 2316 4 -1.2129921431905046 2316 5 -1.4251180001392214 2316 6 -1.4728432468193944 2316 7 -1.6755723233979438 2316 8 -2.090993328796749 2316 9 -1.810443428594013 2316 10 -1.821162172574354 2316 11 -1.6829064831938254 2316 12 -1.90771931259534 2316 13 -1.8651981411748793 2316 14 -1.8253236781278304 2316 15 -1.9513803392166764 2316 16 -2.1130961077642922 2316 17 -2.078236200125808 2316 18 -2.207586794621271 2316 19 -2.0481496616928254 2316 20 -2.154730667948563 2316 21 -2.0442722258665214 2316 22 -2.18003752036487 2316 23 -2.024431130682025 2316 24 -1.9746229881764492 2316 25 -2.0580477306454696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 31752.0 3 1.0 4 555.0 5 5024.0 6 6298.0 7 4908.0 8 4010.0 9 3551.0 10 3542.0 11 3153.0 12 3070.0 13 2982.0 14 2934.0 15 2841.0 16 2940.0 17 3069.0 18 3298.0 19 3704.0 20 4561.0 21 5767.0 22 7408.0 23 9329.0 24 11363.0 25 13304.0 26 15430.0 27 17438.0 28 20534.0 29 24838.0 30 31665.0 31 40848.0 32 52397.0 33 68084.0 34 90226.0 35 121150.0 36 180698.0 37 321784.0 38 573506.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17003269040624 19.78780803966373 13.811460092630703 24.23069917729933 2 15.39315956423053 20.88433074473987 40.18635281590519 23.536156875124416 3 19.18959317110748 24.866634235630716 30.17205332039233 25.771719272869476 4 12.928911247719324 17.600040519163564 38.15238503570752 31.318663197409602 5 13.256143736902601 37.86296401687543 35.81586669800911 13.065025548212864 6 31.663311664218636 37.98194541456169 17.67524832711215 12.679494594107524 7 27.901213336929803 31.798120334848484 22.090188119639897 18.21047820858182 8 25.112693923656714 37.30589966088758 20.2066359553394 17.374770460116302 9 25.931027902862375 15.746524362736034 20.133253865516426 38.18919386888517 10 14.534070844930568 29.740712689683278 34.39923861664749 21.325977848738663 11 34.79583170883683 22.966709502332797 23.80895449957066 18.428504289259713 12 22.66470038787676 25.055802191097325 31.85012385436188 20.429373566664037 13 28.868843943504036 21.77663575509935 25.998873944175426 23.355646357221186 14 20.933801816530643 21.898075457519074 28.041381373670315 29.126741352279968 15 23.20010695174568 30.068635222696383 23.917614175111105 22.81364365044683 16 22.48607448223223 28.72296317585435 26.931403647431452 21.859558694481972 17 21.745127393899274 28.445984067959117 27.730950398183236 22.077938139958373 18 21.57951709166636 28.00469032875883 29.159663172674065 21.256129406900744 19 22.908463204712472 27.35178996938683 28.07006281648235 21.66968400941835 20 22.90481176846125 27.427881189331682 28.586269892965806 21.081037149241265 21 23.018065186382263 26.95619807745992 27.922297436573963 22.103439299583854 22 22.553684947012947 27.48282941549929 28.302341277366633 21.661144360121135 23 22.574121211193184 27.205909201737143 27.999507645047416 22.22046194202226 24 22.504626134153767 27.086766370507704 28.526080088953698 21.88252740638483 25 22.72961782876797 27.181921516199136 28.346398261412425 21.74206239362047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 202.0 1 202.0 2 380.5 3 559.0 4 559.0 5 559.0 6 1404.0 7 2249.0 8 2249.0 9 2249.0 10 3393.5 11 4538.0 12 4538.0 13 4538.0 14 7046.5 15 9555.0 16 9555.0 17 9555.0 18 16182.5 19 22810.0 20 22810.0 21 22810.0 22 34697.0 23 46584.0 24 46584.0 25 46584.0 26 64905.5 27 83227.0 28 83227.0 29 83227.0 30 105041.5 31 126856.0 32 126856.0 33 126856.0 34 143108.5 35 159361.0 36 159361.0 37 159361.0 38 173065.0 39 186769.0 40 186769.0 41 186769.0 42 197874.0 43 208979.0 44 208979.0 45 208979.0 46 213137.0 47 217295.0 48 217295.0 49 217295.0 50 211579.5 51 205864.0 52 205864.0 53 205864.0 54 184787.0 55 163710.0 56 163710.0 57 163710.0 58 140716.5 59 117723.0 60 117723.0 61 117723.0 62 95755.0 63 73787.0 64 73787.0 65 73787.0 66 56679.0 67 39571.0 68 39571.0 69 39571.0 70 28815.5 71 18060.0 72 18060.0 73 18060.0 74 12415.5 75 6771.0 76 6771.0 77 6771.0 78 4636.5 79 2502.0 80 2502.0 81 2502.0 82 1636.0 83 770.0 84 770.0 85 770.0 86 484.0 87 198.0 88 198.0 89 198.0 90 110.0 91 22.0 92 22.0 93 22.0 94 11.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4090786484032034 2 0.0 3 0.0 4 0.0 5 0.034335279588117995 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0018846122586960133 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1697962.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.3873370635334 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28623040953435 61.95440894225137 2 11.736070664405318 17.460300886291293 3 2.8360868530732675 6.329068460430505 4 0.9867532855473837 2.93607797002249 5 0.4253309640555285 1.5819618893378102 6 0.207616258968923 0.9266412381474669 7 0.13371492104086022 0.6962687831323157 8 0.07533343939753714 0.4483083158895889 9 0.05582643308389491 0.3737501725379835 >10 0.23069550561063257 2.979142856505741 >50 0.014384634560876155 0.7468868127010564 >100 0.009898196083614341 1.4778411979772088 >500 0.001266729007820059 0.6620147915254289 >1k 7.125350668987831E-4 1.0476453275016326 >5k 7.917056298875367E-5 0.37968235574810416 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6447 0.37969047599416245 No Hit GGTATCAACGCAGAGTACTTTTTTT 4048 0.23840345072504565 No Hit TATCAACGCAGAGTACTTTTTTTTT 4041 0.23799119179345593 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2121 0.12491445627169513 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.8894133084250416E-5 0.0 0.0 0.0 6 0.0 5.8894133084250416E-5 0.0 0.0 5.8894133084250416E-5 7 0.0 5.8894133084250416E-5 0.0 0.0 5.8894133084250416E-5 8 0.0 5.8894133084250416E-5 0.0 0.0 5.8894133084250416E-5 9 0.0 5.8894133084250416E-5 0.0 0.0 5.8894133084250416E-5 10 0.0 5.8894133084250416E-5 0.0 0.0 5.8894133084250416E-5 11 0.0 5.8894133084250416E-5 0.0 0.0 5.8894133084250416E-5 12 0.0 1.1778826616850083E-4 0.0 0.0 5.8894133084250416E-5 13 0.0 1.1778826616850083E-4 0.0 0.0 5.8894133084250416E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 85 1.7234925E-8 14.525156 19 GGACCGT 70 1.0940025E-4 12.210709 6 GGTATCA 1170 0.0 11.81913 1 TCCAACG 145 9.822543E-11 11.789648 18 TGGACCG 75 2.072866E-4 11.400689 5 TGTAGGA 940 0.0 10.309134 2 TTAGGAC 540 0.0 10.204321 3 TAGACAG 290 0.0 10.1557865 5 TAGGACG 805 0.0 10.149683 4 AACCGGG 85 6.611394E-4 10.055878 14 GGCGAGG 370 0.0 10.01058 19 AGGACGT 790 0.0 9.981616 5 TGGCGAG 790 0.0 9.978088 18 GTCTAAG 220 1.8189894E-12 9.970307 1 TAACCTG 125 2.567538E-6 9.880597 5 GATATAC 580 0.0 9.86567 1 ATAGCCC 175 2.568413E-9 9.772019 3 ACTGTTC 625 0.0 9.725151 8 TAGGACA 385 0.0 9.623958 4 GGACGTG 760 0.0 9.622181 6 >>END_MODULE