FastQCFastQC Report
Thu 26 May 2016
SRR1511689_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511689_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948649
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55160.2830679101264517No Hit
GTCCTACAGTGGACATTTCTAAATT52520.2695200623611538No Hit
CTGTAGGACGTGGAATATGGCAAGA48540.2490956555028638No Hit
GTCCTAAAGTGTGTATTTCTCATTT43960.22559219233427877No Hit
CTTTAGGACGTGAAATATGGCGAGG38760.19890703764505563No Hit
GGTATCAACGCAGAGTACTTTTTTT32900.168834921014508No Hit
TATCAACGCAGAGTACTTTTTTTTT31800.16318998444563387No Hit
GTCCTACAGTGTGCATTTCTCATTT22900.11751731584292502No Hit
TTGTAGAACAGTGTATATCAATGAG22770.11685018697569444No Hit
CTGAAGGACCTGGAATATGGCGAGA20530.10535504341725986No Hit
GATATACACTGTTCTACAAATCCCG20190.10361024484142603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAGT402.8593934E-416.54929213
CGGTAGG807.9329766E-814.826531
TCCAACG3300.013.46868718
GGTATCA10550.012.6482251
GTCCTAT3400.012.2100841
TAGGACC8550.012.0559594
CCAACGA2050.011.99391219
CCTATAG3350.011.5739183
TCCTATA4150.011.4325052
AGGCGAG600.00607959311.03286218
CCAACGT1803.6379788E-1211.03286119
GGCGAGG11750.011.026154519
CCGGTTT2500.010.96981612
GTCCTAC22950.010.8534081
AATGTCC6750.010.6475878
GACCTAC1501.3196768E-810.543311
GACTATC1002.5231378E-510.4024117
CGGTTTC2550.010.3838713
GAAATGT7050.010.3286366
ACTGTTC10550.010.3082938
Produced by FastQC (version 0.11.5)