##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1511689_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1948649 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.61683504828217 31.0 30.0 34.0 25.0 34.0 2 26.938722160840666 31.0 26.0 34.0 10.0 34.0 3 28.623578694777766 31.0 28.0 34.0 16.0 34.0 4 33.138846452080394 35.0 33.0 37.0 28.0 37.0 5 33.603956382088306 35.0 35.0 37.0 28.0 37.0 6 33.86506292308158 37.0 35.0 37.0 30.0 37.0 7 33.91707280274693 37.0 35.0 37.0 30.0 37.0 8 33.936944005821466 37.0 35.0 37.0 30.0 37.0 9 35.55590308978169 39.0 37.0 39.0 30.0 39.0 10 35.432314388070914 39.0 35.0 39.0 30.0 39.0 11 35.45149382982774 39.0 35.0 39.0 30.0 39.0 12 35.36220838129391 39.0 35.0 39.0 30.0 39.0 13 35.36477118249618 39.0 35.0 39.0 30.0 39.0 14 36.51880200077079 40.0 37.0 41.0 30.0 41.0 15 36.472540206060714 40.0 37.0 41.0 30.0 41.0 16 36.421648537012054 40.0 37.0 41.0 29.0 41.0 17 36.40625017640427 40.0 37.0 41.0 30.0 41.0 18 36.35315133715718 40.0 37.0 41.0 29.0 41.0 19 36.39323654490881 40.0 37.0 41.0 29.0 41.0 20 36.32662116163557 40.0 37.0 41.0 29.0 41.0 21 36.245363839254786 40.0 37.0 41.0 29.0 41.0 22 36.14958414778649 40.0 37.0 41.0 29.0 41.0 23 36.04518874358594 40.0 36.0 41.0 28.0 41.0 24 35.95144174245849 40.0 36.0 41.0 28.0 41.0 25 35.85413073365188 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9361348265263274 1101 2 3.5037277484402303 1101 3 2.0491663484613127 1101 4 1.286706689081207 1101 5 0.6513609236157123 1101 6 0.4265419312612977 1101 7 0.3194134272867899 1101 8 0.15072348214236797 1101 9 -2.3759517915777906E-4 1101 10 0.3333111913692832 1101 11 0.14179031657282337 1101 12 0.09044740739206247 1101 13 0.2727832975453168 1101 14 0.4748148264988288 1101 15 0.2548791199309477 1101 16 0.4388020226861684 1101 17 0.44156888950767126 1101 18 0.29132229384338615 1101 19 0.4220211805484979 1101 20 0.5084924288392614 1101 21 0.4911143710330492 1101 22 0.3817483258358294 1101 23 0.47850883217468265 1101 24 0.48364851838820044 1101 25 0.5299787097654018 1102 1 1.23423513808876 1102 2 3.9338069557436732 1102 3 2.431979537694531 1102 4 1.3136193288846485 1102 5 0.8981121373294982 1102 6 0.583272694751777 1102 7 0.47549015509186177 1102 8 0.4584469404655067 1102 9 0.5290305017428238 1102 10 0.6633710296645958 1102 11 0.6994383219961477 1102 12 0.744789547654193 1102 13 0.8043010516955533 1102 14 0.7222054828097058 1102 15 0.7855985658181197 1102 16 0.6935409622860433 1102 17 0.8151144726773296 1102 18 0.6505590222587188 1102 19 0.6459242612157752 1102 20 0.7120232265571644 1102 21 0.6888702857112392 1102 22 0.4963611148204379 1102 23 0.5686990928802018 1102 24 0.577592187740656 1102 25 0.3141558884305766 1103 1 1.2803267531044007 1103 2 3.9786277325156796 1103 3 2.553128961517494 1103 4 1.3944726696922487 1103 5 1.0839214287814443 1103 6 0.7960759919240843 1103 7 0.6491478122904155 1103 8 0.5749378980851176 1103 9 0.8584515218532829 1103 10 0.46404817304954804 1103 11 0.695184358191149 1103 12 0.7546428438709825 1103 13 0.859296178556427 1103 14 0.9132433658925905 1103 15 0.7101642831250885 1103 16 0.7576154162078055 1103 17 0.8630553325156569 1103 18 0.8926979799802552 1103 19 0.9374169706174271 1103 20 0.9895379893021783 1103 21 0.9742960961493097 1103 22 1.0300354617242462 1103 23 0.7370364287700752 1103 24 0.9814327309841957 1103 25 0.5508840691890029 1104 1 1.4153247166426155 1104 2 3.9264809370554694 1104 3 2.4403223524391287 1104 4 1.62157949579057 1104 5 1.122484720190002 1104 6 0.9487832702992947 1104 7 0.8586839245328903 1104 8 0.7786558086145376 1104 9 0.8579469656499796 1104 10 0.9171767254595693 1104 11 0.9503809831356804 1104 12 0.7497699251653174 1104 13 0.8494749616641286 1104 14 0.981854498955606 1104 15 0.9259337921250363 1104 16 0.7827417072333915 1104 17 0.7922089546820885 1104 18 0.7934681880640966 1104 19 0.9098712414121337 1104 20 0.9030388190843652 1104 21 0.8447254552413526 1104 22 0.9586279930766395 1104 23 1.1247866552124677 1104 24 0.9847597624308122 1104 25 0.9259085371253661 1105 1 1.5007764467137967 1105 2 3.8729864169904857 1105 3 2.4493993549843687 1105 4 1.5974319398872723 1105 5 1.0472309283935672 1105 6 0.9071493512760611 1105 7 0.7959080097755447 1105 8 0.7078482780816344 1105 9 0.7796138216770885 1105 10 0.6269228926675723 1105 11 0.7102038120401275 1105 12 0.7704188028825953 1105 13 0.8146750024254246 1105 14 0.7405587347339591 1105 15 0.814260716485883 1105 16 0.8603287320216495 1105 17 0.9365812599561067 1105 18 0.8576004701931979 1105 19 0.789511792345607 1105 20 0.8496585009353979 1105 21 0.8233468565632833 1105 22 0.7895798748313894 1105 23 1.0007897287081349 1105 24 0.9931369122086764 1105 25 0.9693005800043792 1106 1 1.044135062952801 1106 2 3.754047390814076 1106 3 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-1.403415141950596 2315 14 -1.7720763923621021 2315 15 -1.6894871018538353 2315 16 -1.8862731242281399 2315 17 -1.700564720870581 2315 18 -1.6475113835177595 2315 19 -1.7021569119425806 2315 20 -1.7071241555796988 2315 21 -1.7799886596013792 2315 22 -1.757256378155951 2315 23 -1.6214642835325819 2315 24 -1.5831300902205427 2315 25 -1.629219591511827 2316 1 -0.44826494702299513 2316 2 2.127718430634225 2316 3 0.6173924185326456 2316 4 -0.2961551027538363 2316 5 -0.534451473224749 2316 6 -0.7584712427007005 2316 7 -1.1185901617903156 2316 8 -1.0582127610167191 2316 9 -1.360006955818477 2316 10 -1.2563831379744812 2316 11 -1.1364832311133455 2316 12 -1.4232950726758204 2316 13 -1.5052716280715757 2316 14 -1.6377457099143982 2316 15 -1.6497498027745081 2316 16 -1.7257802455410811 2316 17 -1.6232542531675875 2316 18 -1.8056311188886482 2316 19 -1.5827288464488731 2316 20 -1.547489924319585 2316 21 -1.9120949556336342 2316 22 -1.671731893334993 2316 23 -1.9247095565466878 2316 24 -1.953056679631537 2316 25 -1.8200801558314836 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58035.0 3 1.0 4 489.0 5 2551.0 6 3056.0 7 2792.0 8 2625.0 9 2538.0 10 2405.0 11 2369.0 12 2317.0 13 2218.0 14 2339.0 15 2370.0 16 2503.0 17 2780.0 18 3160.0 19 3892.0 20 5174.0 21 6482.0 22 8105.0 23 9706.0 24 11328.0 25 13127.0 26 15183.0 27 16952.0 28 20089.0 29 24506.0 30 31218.0 31 40637.0 32 53159.0 33 71572.0 34 99589.0 35 144621.0 36 234307.0 37 301525.0 38 742929.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.095677569434 19.547132397881814 13.479749303235216 24.877440729448967 2 15.515881913102994 21.214200122081913 41.795891482265404 21.47402648254969 3 18.715325335655624 24.97843377642664 30.030498052753472 26.275742835164262 4 11.563806514154166 17.365928907668852 39.82102472020358 31.249239857973393 5 12.37082717308248 38.323217778060595 36.7297035022726 12.576251546584327 6 30.22468387072274 38.55594311751373 18.82206595441252 12.397307057351016 7 26.88354855081649 31.896252223976713 23.02867268553752 18.19152653966928 8 23.410424350408924 39.311030360008395 21.13161477515961 16.14693051442307 9 25.7427581878522 15.781189942365199 21.048069713940272 37.42798215584233 10 14.172485655446415 29.931147169141287 35.538827156660844 20.35754001875145 11 33.62119088660913 22.670629754255383 25.82286496952504 17.885314389610443 12 22.96201111641963 24.753560030564767 33.2976333860023 18.9867954670133 13 28.845471914131277 22.58010549873271 26.32459719528761 22.249825391848404 14 19.797203087882938 23.269865429843957 28.53299901624151 28.399932466031597 15 21.946846250915378 32.685927532357034 23.900507479797543 21.466718736930048 16 20.689667559421938 28.77393517252209 29.797464807669314 20.738932460386657 17 20.487732783071756 29.344176401188722 29.180935099137916 20.987155716601606 18 20.20030287650572 28.18167869123685 31.907234191483436 19.710784240773993 19 22.740267744473222 27.09292437991655 28.389874215417965 21.776933660192267 20 22.49517486217374 28.32162180053976 29.49653837094315 19.686664966343347 21 21.920348796035814 27.525060991067647 28.06748891281433 22.48710130008221 22 21.81741951144162 29.37867661963707 28.648779579099376 20.15512428982193 23 21.27240732881496 28.63232095049001 28.989904225363677 21.10536749533135 24 22.193552757374732 27.440013506807542 29.97100039155373 20.395433344263996 25 21.855654866525477 28.164230705478516 29.42305156033744 20.55706286765857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 177.0 2 350.5 3 524.0 4 524.0 5 524.0 6 1521.0 7 2518.0 8 2518.0 9 2518.0 10 3703.5 11 4889.0 12 4889.0 13 4889.0 14 7897.0 15 10905.0 16 10905.0 17 10905.0 18 19084.0 19 27263.0 20 27263.0 21 27263.0 22 43463.0 23 59663.0 24 59663.0 25 59663.0 26 86550.5 27 113438.0 28 113438.0 29 113438.0 30 148008.5 31 182579.0 32 182579.0 33 182579.0 34 199819.5 35 217060.0 36 217060.0 37 217060.0 38 225805.5 39 234551.0 40 234551.0 41 234551.0 42 241571.5 43 248592.0 44 248592.0 45 248592.0 46 247850.0 47 247108.0 48 247108.0 49 247108.0 50 231708.0 51 216308.0 52 216308.0 53 216308.0 54 186853.5 55 157399.0 56 157399.0 57 157399.0 58 131294.5 59 105190.0 60 105190.0 61 105190.0 62 84505.0 63 63820.0 64 63820.0 65 63820.0 66 48631.5 67 33443.0 68 33443.0 69 33443.0 70 24094.5 71 14746.0 72 14746.0 73 14746.0 74 10169.0 75 5592.0 76 5592.0 77 5592.0 78 3794.5 79 1997.0 80 1997.0 81 1997.0 82 1300.0 83 603.0 84 603.0 85 603.0 86 398.0 87 193.0 88 193.0 89 193.0 90 128.5 91 64.0 92 64.0 93 64.0 94 41.0 95 18.0 96 18.0 97 18.0 98 13.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.327459691304078 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.5395281551474894E-4 22 9.237168930884936E-4 23 6.158112620589958E-4 24 1.0263521034316596E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1948649.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.00517242097366 #Duplication Level Percentage of deduplicated Percentage of total 1 87.4703941565755 65.60731995444472 2 9.219826134409855 13.830692978056206 3 1.8191166977853996 4.093294847137984 4 0.5851436429346132 1.7555519931738923 5 0.25235899027513276 0.9464114788784573 6 0.1370089048850793 0.6165825920468492 7 0.09077374339835313 0.47659501924234876 8 0.06944067014964495 0.41667275500816625 9 0.049949230192179436 0.337180556057339 >10 0.26086048090632824 3.557172839492071 >50 0.023320644660059356 1.2228727827633197 >100 0.017585175221139955 2.6796975993592023 >500 0.002145366996868839 1.1179863161306873 >1k 0.001937750835881515 2.7830270377633215 >5k 1.3841077399153677E-4 0.5589412504454992 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5516 0.2830679101264517 No Hit GTCCTACAGTGGACATTTCTAAATT 5252 0.2695200623611538 No Hit CTGTAGGACGTGGAATATGGCAAGA 4854 0.2490956555028638 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4396 0.22559219233427877 No Hit CTTTAGGACGTGAAATATGGCGAGG 3876 0.19890703764505563 No Hit GGTATCAACGCAGAGTACTTTTTTT 3290 0.168834921014508 No Hit TATCAACGCAGAGTACTTTTTTTTT 3180 0.16318998444563387 No Hit GTCCTACAGTGTGCATTTCTCATTT 2290 0.11751731584292502 No Hit TTGTAGAACAGTGTATATCAATGAG 2277 0.11685018697569444 No Hit CTGAAGGACCTGGAATATGGCGAGA 2053 0.10535504341725986 No Hit GATATACACTGTTCTACAAATCCCG 2019 0.10361024484142603 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.131760517158298E-5 0.0 3 0.0 0.0 0.0 5.131760517158298E-5 0.0 4 0.0 0.0 0.0 5.131760517158298E-5 0.0 5 0.0 0.0 0.0 5.131760517158298E-5 0.0 6 0.0 0.0 0.0 5.131760517158298E-5 0.0 7 0.0 0.0 0.0 5.131760517158298E-5 0.0 8 0.0 0.0 0.0 5.131760517158298E-5 0.0 9 0.0 0.0 0.0 1.0263521034316596E-4 0.0 10 0.0 0.0 0.0 1.0263521034316596E-4 0.0 11 0.0 0.0 0.0 1.0263521034316596E-4 0.0 12 0.0 0.0 0.0 1.0263521034316596E-4 5.131760517158298E-5 13 0.0 0.0 0.0 2.0527042068633193E-4 5.131760517158298E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAAGT 40 2.8593934E-4 16.549292 13 CGGTAGG 80 7.9329766E-8 14.82653 1 TCCAACG 330 0.0 13.468687 18 GGTATCA 1055 0.0 12.648225 1 GTCCTAT 340 0.0 12.210084 1 TAGGACC 855 0.0 12.055959 4 CCAACGA 205 0.0 11.993912 19 CCTATAG 335 0.0 11.573918 3 TCCTATA 415 0.0 11.432505 2 AGGCGAG 60 0.006079593 11.032862 18 CCAACGT 180 3.6379788E-12 11.032861 19 GGCGAGG 1175 0.0 11.0261545 19 CCGGTTT 250 0.0 10.969816 12 GTCCTAC 2295 0.0 10.853408 1 AATGTCC 675 0.0 10.647587 8 GACCTAC 150 1.3196768E-8 10.54331 1 GACTATC 100 2.5231378E-5 10.402411 7 CGGTTTC 255 0.0 10.38387 13 GAAATGT 705 0.0 10.328636 6 ACTGTTC 1055 0.0 10.308293 8 >>END_MODULE