FastQCFastQC Report
Thu 26 May 2016
SRR1511689_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1511689_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948649
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66300.34023572228759513No Hit
GTCCTACAGTGGACATTTCTAAATT54460.27947567776444093No Hit
CTGTAGGACGTGGAATATGGCAAGA52940.27167540177836025No Hit
GTCCTAAAGTGTGTATTTCTCATTT45510.23354642113587415No Hit
CTTTAGGACGTGAAATATGGCGAGG41810.21455890722238843No Hit
TATCAACGCAGAGTACTTTTTTTTT41710.2140457311706726No Hit
GGTATCAACGCAGAGTACTTTTTTT40770.2092218762845438No Hit
TTGTAGAACAGTGTATATCAATGAG26840.13773645228052872No Hit
GTCCTACAGTGTGCATTTCTCATTT24420.12531759182900565No Hit
CTGAAGGACCTGGAATATGGCGAGA24180.12408596930488765No Hit
GATATACACTGTTCTACAAATCCCG22920.11761995105326818No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20030.10278916315868071No Hit
CTGTAGGACCTGGAATATGGCGAGA19940.10232730471213647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8150.013.9884864
GGTATCA11000.013.006661
AGCGTAC701.0510287E-412.2634231
ACTGTTC9800.011.7260128
ACCTTTT7400.011.67886715
GACAGTC1552.5465852E-1111.6416077
GCGTACA752.073289E-411.4006162
CCAACGA2600.011.32404719
CACCTTT7650.011.29720514
TCCAACG3450.011.28640918
GGCGAGG10000.011.11217719
GGACCTG15800.011.0599156
GATATAC10400.011.0056361
AGGACCT16850.010.9946595
GTTTAGG2350.010.9588041
CTATACT2700.010.9079974
CTAGGAC3750.010.8939223
AATCCCG5450.010.80459619
GTATCAA23800.010.780591
TGTAGGA30900.010.7610992
Produced by FastQC (version 0.11.5)